2013 Fiscal Year Final Research Report
Screening of food factors that target the quality control system of mitochondria
| Project/Area Number |
23658118
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Food science
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | オートファジー / ポリフェノール / フラボノイド / ミトコンドリア |
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| Research Abstract |
We screened the food factors that enhance the autophagic degradation leading to mitochondrial quality control. In HeLa cells, luteolin and the derivatives significantly enhanced the autophagic degradation of p62, an adaptor protein of autophagy. In contrast, in J774.1 macrophages, the mRNA expression of p62 was significantly enhanced upon treatment with flavonols such as quercetin, whereas no effects were observed upon treatment with luteolin derivatives. These results strongly suggest that the activities of polyphenols for autophagy induction are dependent on cell types. It was also found that p62 mRNA expression was enhanced through the activation of Nrf2, a transcription factor. Furthermore, the pretreatment of J774.1 with flavonols resulted in the decrease in both intracellular and extracellular IL-1beta, an autophagy substrate. These results suggest that autophagy induction by polyphenols might be good strategy for the quality control of intracellular proteins and organelles.
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[Journal Article] Mitochondrial dysfunction leads to deconjugation of quercetin glucuronides in inflammatory macrophages2013
Author(s)
Ishisaka, A., Kawabata, K., Miki, S., Shiba, Y., Minekawa, S., Nishikawa, T., Mukai, R., Terao, J., and Kawai, Y
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Journal Title
PLoS One
Volume: 8
Pages: e80843
DOI
Peer Reviewed
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[Journal Article] Non-specific protein modifications by a phytochemical induce heat shock response for self-defense2013
Author(s)
Ohnishi, K., Ohkura, S., Nakahata, E., Ishisaka, A., Kawai, Y., Terao, J., Mori, T., Ishii, T., Nakayama, T., Kioka, N., Matsumoto, S., Ikeda, Y., Akiyama, M., Irie, K., and Murakami, A
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Journal Title
PLoS One
Volume: 8
Pages: e58641
DOI
Peer Reviewed
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[Journal Article] Atg7 modulates p53 activity to regulate cell cycle and survival during metabolic stress2012
Author(s)
Lee, I.H., Kawai, Y., Fergusson, M.M., Rovira, I.I., Bishop, A.J., Motoyama, N., Cao, L., and Finkel, T
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Journal Title
Science
Volume: 336
Pages: 225-228
DOI
Peer Reviewed
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[Journal Article] Bioavailability of orally administered water-dispersible hesperetin and its effect on peripheral vasodilatation in human subjects : implication of endothelial functions of plasma conjugated metabolites2012
Author(s)
Takumi, H., Nakamura, H., Simizu, T., Harada, R., Kometani, T., Nadamoto, T., Mukai, R., Murota, K., Kawai, Y., and Terao, J
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Journal Title
Food Funct
Volume: 3
Pages: 389-398
DOI
Peer Reviewed
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[Journal Article] Effect of quercetin and its glucuronide metabolite upon 6-hydorxydopamine-induced oxidative damage in Neuro-2a cells2012
Author(s)
Mukai, R., Kawabata, K., Otsuka, S., Ishisaka, A., Kawai, Y., Ji, Z.S., Tsuboi, H., and Terao, J
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Journal Title
Free Radic Res
Volume: 46
Pages: 1019-28
DOI
Peer Reviewed
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