2013 Fiscal Year Final Research Report
Molecular mechanism of site-specificity of Caligus fugu on fins of puffer fish
| Project/Area Number |
23658162
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General fisheries
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| Research Institution | Hiroshima University |
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Principal Investigator |
OHTSUKA Susumu 広島大学, 生物圏科学研究科, 教授 (00176934)
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| Co-Investigator(Kenkyū-buntansha) |
TASUMI Satoshi 東京大学, 大学院・農学生命科学研究科, 特任助教 (90359646)
SUZUKI Yuzuru 東京大学, 大学院・農学生命科学研究科, 教授 (40107412)
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| Project Period (FY) |
2011 – 2013
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| Keywords | トラフグ類 / ウオジラミ類 / 宿主特異性 / 寄生部位特異性 / 鰭 / サブトラクティブPCR法 / カワハギ類 / ペンネラ科 |
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| Research Abstract |
(1) The life cycle and developmental stages of some parasitic copepods have been newly revealed by the present investigation. The number of post-naupliar stages of caligids is evidently six. Reinfection of adults is implied in some caligids. The whole life cycle of a pennelid Peniculus minuticaudae infecting fins of filefish is totally disclosed: the hatching stage is copepodids; no host switching is found. It is likely that fish fins are feasibly infected by infective stages of parasitic copepods but so limited for fecundity of adult females. (2) Results of bioassay indicate that attractants for the infective copepodid of Caligus fugu is water-soluble. At least 10 genes coding secretory proteins or enzymes were obtained by suppression subtractive hybridization, which was applied to isolate genes expressed higher in fins compared to skin. These are likely to be candidate genes related to the attachment site-specificity of the infective stage.
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[Presentation] Candidate key molecule(s) determining host specificity of parasite on fugu, Takifugu rubripes2012
Author(s)
S. Tasumi, A. Yamaguchi, Y. Hirabayashi, S. Kido, K. Kobayashi, W. Kai, S. Hosoya, S. Tsutsui, O. Nakamura, H. Suetake, K. Kikuchi, Y. Suzuki
Organizer
The 12th Congress of ISDCI
Place of Presentation
福岡市
Year and Date
2012-07-10
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