2013 Fiscal Year Final Research Report
Research on the identification of growth-stimulating peptides from the ovarian envelopes of salmon
| Project/Area Number |
23658167
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General fisheries
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 卵巣外皮 / サケ / ペプチド / 成長促進 / インスリン様成長因子 |
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| Research Abstract |
Chum salmon is one of the most important aquatic resources in Tohoku and Hokkaido areas of Japan. The salted salmon roe or ikra is high in commercial value, but the ovarian envelopes after removing ikra don't have much utility value. To demonstrate the presence and functions of growth-stimulating peptide in the ovarian envelopes, we tried to identify a peptide involved in growth regulation from ovarian envelopes of salmon.
The 80% acetone extracts of the ovarian envelopes was fractionated by gel filtration chromatography, and peptides were purified by HPLC. At least 5 peptides were purified. Molecular weight of these peptides was estimated from 6.7 to 17.5 kDa, but amino acid sequence of these peptides was not determined. A 10.9 kDa peptide stimulates hepatic Insulin-like growth factor-I mRNA expression of rainbow trout. These results suggest that the 10.9kDa peptide might be involved in growth regulation of salmon.
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Research Products
(17 results)
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[Journal Article] Involvement of insulin-like peptide in long-term synaptic plasticity and long-term memory of the pond snail Lymnaea stagnalis2013
Author(s)
Murakami J, Okada R, Sadamoto H, Kobayashi S, Mita K, Sakamoto Y, Yamagishi M, Hatakeyama D, Otsuka E, Okuta A, Sunada H, Takigami S, Sakakibara M, Fujito Y, Awaji M, Moriyama S, Lukowiak K, Ito E
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Journal Title
J Neurosc
Volume: 33
Pages: 371-381
DOI
Peer Reviewed
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[Journal Article] Chum salmon fry grow faster in seawater, exhibit greater activity of the GH/IGF axis, higher Na^+, K^+-ATPase activity, and greater gill chloride cell development2012
Author(s)
Iwata M, Kinoshita K, Moriyama S, Kurosawa T, Iguma K, Chiba H, Ojima D, Yoshinaga T, Arai T
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Journal Title
Aquaculture
Volume: 362-363
Pages: 101-108
DOI
Peer Reviewed
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[Journal Article] Effects of growth hormone on the salmon pituitary proteome2012
Author(s)
Kurata Y, Kimura Y, Yamanaka Y, Ishikawa A, Okamoto H, Masaoka T, Nagoya H, Araki K, Moriyama S, Hirano H, Mori T
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Journal Title
J Proteomics
Volume: 75
Pages: 1718-1731
DOI
Peer Reviewed
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[Journal Article] Physiological and molecular endocrine changes in maturing wild sockeye salmon, Oncorhynchus nerka, during ocean and river migration2012
Author(s)
Flores AM, Shrimpton JM, Patterson DA, Hills JA, Cooke SJ, Yada T, Moriyama S, Hinch SG, Farrell AP
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Journal Title
J Comp Physiol B
Volume: 182
Pages: 77-90
DOI
Peer Reviewed
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