2011 Fiscal Year Final Research Report
Elucidation of the tetrodotoxin biosynthesis pathway by whole genome analysis of tetrodotoxin producing bacteria
| Project/Area Number |
23658173
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Fisheries chemistry
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
WATABE Shugo 東京大学, 大学院・農学生命科学研究科, 教授 (40111489)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
ASAKAWA Shuichi 東京大学, 大学院・農学生命科学研究科, 教授 (30231872)
|
|---|
| Research Collaborator |
MATSUMOTO Takuya , 日本学術振興会・特別研究員・PD
KIKUCHI Ryosuke 東京大学, 大学院・農学生命科学研究科, 修士課程
HOLGER Feroudj 東京大学, 大学院・農学生命科学研究科, 修士課程
|
|---|
| Project Period (FY) |
2011
|
| Keywords | フグ毒 / テトロドトキシン / フグ毒産生菌 / Vibrio alginolyticus / 生合成経路 |
|---|
| Research Abstract |
The purpose of this study was to elucidate the biosynthesis pathway of pufferfish toxin, tetrodotoxin(TTX), in TTX-producing marine bacterium Vibrio alginolyticus by the whole genome analysis. We identified 6 strains of V. alginolyticus from intestinal flora of pufferfish and TTX was detected from 2 strains by MALDI-TOF-MS analysis. Genomic DNA samples extracted from the TTX-producing V. alginolyticus strains were subjected to whole genome analysis by the next-generation sequencing technology.
|
Research Products
(8 results)
-
[Presentation] Expression analysis of genes related to tetrodotoxin pharmacokinetics in ovary tissues of wild pufferfish Takifugu rubripes2011
Author(s)
H. Feroudj, T. Matsumoto, R. Kikuchi, Y. Kawana, H. Kondo, I. Hirono, S. Aki, T. Mochizuki, Y. Nagashima, G. Kaneko, H. Ushio, M. Kodama, S. Watabe
Organizer
The international symposium on muscle biochemistry
Place of Presentation
東京大学農学部(東京都文京区)
Year and Date
2011-10-28
-
[Presentation] Microarray analysis on the liver of cultured marine pufferfish Takifugu rubripes after the intramuscular administration of tetrodotoxin2011
Author(s)
T. Matsumoto, H. Feroudj, R. Kikuchi, Y. Kawana, H. Kondo, I. Hirono, S. Aki, T. Mochizuki, Y. Nagashima, G. Kaneko, H. Ushio, M. Kodama, S. Watabe
Organizer
The international symposium on muscle biochemistry
Place of Presentation
東京大学農学部(東京都文京区)
Year and Date
2011-10-28
-
-
-
-
[Presentation] Microarray analysis of tetrodotoxin accumulation-related genes in the ovary of wild pufferfish Takifugu rubripes2011
Author(s)
Feroudj Holger,松本拓也,菊地遼輔,川名由利子,近藤秀裕,廣野育生,安藝真一郎,望月俊孝,長島裕二,金子元,潮秀樹,児玉正昭,渡部終五
Organizer
平成23年度日本水産学会秋季大会
Place of Presentation
長崎大学水産学部(長崎県長崎市)
Year and Date
2011-09-29
-
-
