2012 Fiscal Year Final Research Report
Challenging research for functional analyses of piRNA/PIWI in mammalian female germ cells
| Project/Area Number |
23658221
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied animal science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NAITO Kunihiko 東京大学, 農学生命科学研究科, 教授 (20188858)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 繁殖 / 卵成熟 / PIWI / piRNA |
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| Research Abstract |
At least two PIWI mRNA, PIWIL1 and PIWIL2, are present in porcine oocytes and the inhibition of these protein expression results in the meiotic resumption of oocytes under a high cAMP concentration, suggesting the physiological involvement of PIWI in the meiotic arrest of porcine ovarian oocytes. This PIWI function is mediated by the inhibition of cyclin B accumulation and might affect on the APC function, which works on the cyclin B degradation, as no differences are detected in the translational regulations, such as cyclin B mRNA level, Aurora A and CPEB status.
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