Identification of factors that involved in migration of mesenchymal stem cells to lesions of neurodegenerative diseases

2012 Fiscal Year Final Research Report

• Project/Area Number: 23658233
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Hokkaido University
• Principal Investigator: HORIUCHI Motohiro 北海道大学, 大学院・獣医学研究科, 教授 (30219216)
• Project Period (FY): 2011 – 2012
• Keywords: 骨髄由来間葉系幹細胞 / プリオン / 神経変性疾患 / 走化性 / ケモカイン

Research Abstract

In vitro migration assay was carried out to investigate chemoattractive factors for MSCs in the brain lesions of prion-infected mice. In addition, expression of chemokine receptor on MSCs that had been transplanted in the brain of prion-infected mice was analyzed. The combined in vitro and in vivo analyses suggest that CCR3, CCR5, CXCR3 and CXCR4 and their corresponding ligands are involved in the migration of hMSCs to the brain lesions caused by prion propagation.

Research Products

• [Journal Article] Therapeutic effect of peripheral administration of an anti-prion protein antibody on mice infected with prions (2013)
  • Author(s): Ohsawa, N., Song, C.-H., Suzuki, A., Furuoka, H., Hasebe, R., and Horiuchi,M
  • Journal Title: Microbiol. Immunol Volume: 57(4) Pages: 288-297
  • Peer Reviewed
• [Journal Article] Characterization of intracellular localization of PrPSc in prion-infected cells using monoclonal antibody that recognizes the region consisting of amino acids 119-127 of mouse (2012)
  • Author(s): Yamasaki, T., Suzuki, A., Shimizu, T., Watarai, M., Hasebe, R. and Horiuchi, M
  • Journal Title: PrP. J. Gen. Virol Volume: 93(Pt3) Pages: 668-680
  • Peer Reviewed
• [Journal Article] Reaction of complement factors varies with prion strains in vitro and in vivo (2012)
  • Author(s): Hasebe, R., Raymond, G. J., Horiuchi, M., and Caughey, B
  • Journal Title: Virology Volume: 423(2) Pages: 205-213
  • Peer Reviewed
• [Journal Article] Identification of chemoattractive factors involved in the migration of bone marrow-derived mesenchymal stem cells to brain lesions caused by prions (2011)
  • Author(s): Song C.-H., Honmou, O., Furuoka, H. and Horiuchi, M
  • Journal Title: J. Virol Volume: 85(21) Pages: 11069-11078
  • Peer Reviewed
• [Presentation] Construction of mAb132-EGFP fusion proteins as a PrPSc-specific probe (2012)
  • Author(s): Suzuki, A., Yamasaki, T., Hasebe, R., and Horiuchi, M
  • Organizer: Asia Pacific Prion Symposium 2012
  • Place of Presentation: Yokoyama,Japan
  • Year and Date: 20120729-30
• [Presentation] Analysis of PrPSc accumulation and glial cell activationin brains of prion-infected mice at the early stage of infection (2012)
  • Author(s): Horiuchi, M, Yamasaki, T., Hasebe, R. and Takahashi, Y
  • Organizer: Prion2012
  • Place of Presentation: Amsterdam, The Netherland)UV University Amsterdam
  • Year and Date: 20120510-12
• [Presentation] Detection of newly generataed PrPSc in Neuro2a cells inoculated with fluorescent-dye labeled purified PrPSc (2011)
  • Author(s): Yamasaki, T., Garon, G. B., and Horiuchi, M
  • Organizer: XV International Congress of Virology
  • Place of Presentation: Sapporo, Japan Sapporo Convention Center
  • Year and Date: 20110911-16
• [Presentation] Immunohistochemical analysis of PrPSc accumulation and activation of astrosytes and microglia in early stage of prion infection (2011)
  • Author(s): Takahashi, Y., Song, C.-H., Yamasaki, T., Hasebe, R., and Horiuchi, M
  • Organizer: APPS2011
  • Place of Presentation: Karuizawa,Japan Hotel Marroad Karuizawa
  • Year and Date: 20110710-11
• [Book] プリオンの感染と細胞内動態 (2013)
  • Author(s): 山崎剛士、堀内基広
  • Total Pages: 215-224
  • Publisher: Dementia Japan
• [Book] プリオン病最新医学 (2011)
  • Author(s): 堀内基広
  • Total Pages: 2713-2720