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2012 Fiscal Year Final Research Report

Bacterial genetic determinants that control horizontal transfer of genes for degradation of environmental pollutants

Research Project

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Project/Area Number 23658268
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Boundary agriculture
Research InstitutionTohoku University

Principal Investigator

TSUDA Masataka  東北大学, 大学院・生命科学研究科, 教授 (90172022)

Co-Investigator(Kenkyū-buntansha) NAGATA Yuji  東北大学, 大学院・生命科学研究科, 准教授 (30237531)
Project Period (FY) 2011 – 2012
Keywords環境修復 / 環境細菌 / プラスミド / 遺伝子水平伝播 / 接合伝達
Research Abstract

Conjugative transfer of bacterial plasmids plays an important role in rapid adaptation and evolution of host cells. However, the factors governing the host range of transfer, i.e., limited transfer to specific recipient strains within a species, remain unclear. In this study, such factors were investigated using the two plasmids encoding the traits for degradation of environmental pollutants, and the mating pair-formation in the conjugation process and a regulatory system for various metabolisms in the recipient cells were found to be involved in restricting the conjugative transfer of the plasmids.

  • Research Products

    (17 results)

All 2013 2012 2011 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (10 results) Book (1 results)

  • [Journal Article] Cointegrate-resolutionof toluene-catabolic transposon Tn46512013

    • Author(s)
      H. Yano, H. Genka, Y. Ohtsubo, Y. Nagata, E. M. Top, and M. Tsuda
    • Journal Title

      determination of crossover site and the segment required for full resolution activity

      Volume: 69 Pages: 24-35

    • Peer Reviewed
  • [Journal Article] ParI, an orphan ParA family protein from Pseudomonas putida KT2440-specific genomic island,interferes with the partition system of IncP-7 plasmids2012

    • Author(s)
      M. Miyakoshi, M. Shintani, K. Inoue, T. Terabayashi, F. Sai, M. Ohkuma, H. Nojiri, Y. Nagata, and M. Tsuda
    • Journal Title

      Environ. Microbiol

      Volume: 14 Pages: 2946-2959

    • Peer Reviewed
  • [Journal Article] Complete genome sequence of Acidovorax sp. KKS102, a polychlorinated biphenyl-degrading strain2012

    • Author(s)
      Y. Ohtsubo, F. Maruyama, H. Mitsui, Y. Nagata, and M. Tsuda
    • Journal Title

      J. Bacteriol

      Volume: 194 Pages: 6970-6971

    • Peer Reviewed
  • [Journal Article] Conjugal transfer of polychlorinated biphenyl/biphenyl degradation genes in Acidovorax sp. KKS102 located on integrative and conjugative element2012

    • Author(s)
      Y. Ohtsubo, Y. Ishibashi, H. Naganawa, S.Hirokawa, S. Atobe, Y. Nagata, and M. Tsuda
    • Journal Title

      J, Bacteriol

      Volume: 184 Pages: 4237-4248

    • Peer Reviewed
  • [Journal Article] Genomic organizationand genomic structural rearrangements of Sphingobium japonicum UT26, an archetypal gamma-hexachlorocyclohexane-degrading bacterium2011

    • Author(s)
      Y. Nagata, S. Natsui, R. Endo, Y. Ohtsubo, N. Ichikawa, A. Ankai, A. Oguchi, S. Fukui, N. Fujita, and M. Tsuda
    • Journal Title

      Enzyme Microb. Technol

      Volume: 49 Pages: 499-508

    • Peer Reviewed
  • [Journal Article] The lin genes for gamma-hexachlorocyclohexane degradation in Sphingomonas sp. MM-1 proved to be dispersed across multiple plasmids2011

    • Author(s)
      M. Tabata, R. Endo, M. Ito, Y. Ohtsubo, A. Kumar, M. Tsuda, and Y. Nagata
    • Journal Title

      Biosci. Biotechnol. Biochem

      Volume: 75 Pages: 466-472

    • Peer Reviewed
  • [Presentation] プラスミド保持細菌のプラスミドに対する応答の比較2013

    • Author(s)
      高橋裕里香,3人,津田雅孝,野尻秀昭
    • Organizer
      日本農芸化学会2013年大会
    • Place of Presentation
      仙台
    • Year and Date
      20130324-27
  • [Presentation] IncP-9群プラスミドNAH7の接合伝達開始配列の解析2013

    • Author(s)
      岸田康平,井上慧,宮崎亮,大坪嘉行,永田裕二,津田雅孝
    • Organizer
      第7回日本ゲノム微生物学会年会
    • Place of Presentation
      長浜
    • Year and Date
      20130308-10
  • [Presentation] Multiple plasmids ofγ-hexachlorocylohexane-degrading bacterium Sphingomonas sp2012

    • Author(s)
      M. Tabata, S. Ohhata, Y. Ohtsubo, Y. Nagata, and M. Tsuda
    • Organizer
      MM-1. International Plasmid Biology Conference 2012
    • Place of Presentation
      Santander, Spain
    • Year and Date
      20120911-16
  • [Presentation] Relationship between intergeneric conjugative transfer of plasmid NAH7 and nitrogen-related phosphotransferase system2012

    • Author(s)
      K. Inoue, R. Miyazaki, Y. Ohtsubo, Y. Nagata, and M. Tsuda
    • Organizer
      International Plasmid Biology Conference 2012
    • Place of Presentation
      Santander, Spain
    • Year and Date
      20120911-16
  • [Presentation] 環境汚染物質分解能を担うプラスミドの水平伝達2012

    • Author(s)
      津田雅孝
    • Organizer
      日本農芸化学会2012年大会
    • Place of Presentation
      京都
    • Year and Date
      20120322-26
  • [Presentation] ナフタレン分解プラスミドNAH7の接合伝達関連遺伝子群のsinglecellレベルでの発現解析2012

    • Author(s)
      井上慧,宮崎亮,大坪嘉行,永田裕二,津田雅孝
    • Organizer
      日本農芸化学会2012年大会
    • Place of Presentation
      京都
    • Year and Date
      20120322-26
  • [Presentation] IncP-7群プラスミドの接合伝達宿主域を規定する因子の探索2012

    • Author(s)
      瀬谷学人,井上慧,大坪嘉行,永田裕二,津田雅孝
    • Organizer
      日本農芸化学会2012年大会
    • Place of Presentation
      京都
    • Year and Date
      20120322-26
  • [Presentation] Nitrogen-related phosphotransferase system of Pseudomonas putida KT2440 exerts negative effect onconjugative acquisition ofnaphthalene-catabolic plasmid NAH72011

    • Author(s)
      K. Inoue, R. Miyazaki, Y. Ohtsubo, Y. Nagata, and M. Tsuda
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      Sapporo
    • Year and Date
      20110906-10
  • [Presentation] Structural analysis of plasmids for gamma-hexachlorocyclohexanedegradation in Sphingomonas sp2011

    • Author(s)
      M. Tabata, S. Ohhata, Y. Ohtsubo, Y. Nagata, and M. Tsuda
    • Organizer
      MM-1. International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      Sapporo
    • Year and Date
      20110906-10
  • [Presentation] ナフタレン分解プラスミドNAH7の接合伝達制御に関与する受容菌のリン酸基転移系2011

    • Author(s)
      井上慧,大坪嘉行,永田裕二,津田雅孝
    • Organizer
      ゲノム微生物学会ワークショップ
    • Place of Presentation
      仙台
    • Year and Date
      20110820-21
  • [Book] Mobile catabolic genetic elements in pseudomonads. In: Nojiri, H., M. Tsuda, M. Fukuda, and Y. Kamagata (eds), Biodegradative Bacteria

    • Author(s)
      M. Tsuda, Y. Ohtsubo, and H. Yano
    • Publisher
      Springer Verlag, Tokyo

URL: 

Published: 2014-09-25  

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