Bacterial genetic determinants that control horizontal transfer of genes for degradation of environmental pollutants

2012 Fiscal Year Final Research Report

• Project/Area Number: 23658268
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Boundary agriculture
• Research Institution: Tohoku University
• Principal Investigator: TSUDA Masataka 東北大学, 大学院・生命科学研究科, 教授 (90172022)
• Co-Investigator(Kenkyū-buntansha): NAGATA Yuji 東北大学, 大学院・生命科学研究科, 准教授 (30237531)
• Project Period (FY): 2011 – 2012
• Keywords: 環境修復 / 環境細菌 / プラスミド / 遺伝子水平伝播 / 接合伝達

Research Abstract

Conjugative transfer of bacterial plasmids plays an important role in rapid adaptation and evolution of host cells. However, the factors governing the host range of transfer, i.e., limited transfer to specific recipient strains within a species, remain unclear. In this study, such factors were investigated using the two plasmids encoding the traits for degradation of environmental pollutants, and the mating pair-formation in the conjugation process and a regulatory system for various metabolisms in the recipient cells were found to be involved in restricting the conjugative transfer of the plasmids.

Research Products

• [Journal Article] Cointegrate-resolutionof toluene-catabolic transposon Tn4651 (2013)
  • Author(s): H. Yano, H. Genka, Y. Ohtsubo, Y. Nagata, E. M. Top, and M. Tsuda
  • Journal Title: determination of crossover site and the segment required for full resolution activity Volume: 69 Pages: 24-35
  • Peer Reviewed
• [Journal Article] ParI, an orphan ParA family protein from Pseudomonas putida KT2440-specific genomic island,interferes with the partition system of IncP-7 plasmids (2012)
  • Author(s): M. Miyakoshi, M. Shintani, K. Inoue, T. Terabayashi, F. Sai, M. Ohkuma, H. Nojiri, Y. Nagata, and M. Tsuda
  • Journal Title: Environ. Microbiol Volume: 14 Pages: 2946-2959
  • Peer Reviewed
• [Journal Article] Complete genome sequence of Acidovorax sp. KKS102, a polychlorinated biphenyl-degrading strain (2012)
  • Author(s): Y. Ohtsubo, F. Maruyama, H. Mitsui, Y. Nagata, and M. Tsuda
  • Journal Title: J. Bacteriol Volume: 194 Pages: 6970-6971
  • Peer Reviewed
• [Journal Article] Conjugal transfer of polychlorinated biphenyl/biphenyl degradation genes in Acidovorax sp. KKS102 located on integrative and conjugative element (2012)
  • Author(s): Y. Ohtsubo, Y. Ishibashi, H. Naganawa, S.Hirokawa, S. Atobe, Y. Nagata, and M. Tsuda
  • Journal Title: J, Bacteriol Volume: 184 Pages: 4237-4248
  • Peer Reviewed
• [Journal Article] Genomic organizationand genomic structural rearrangements of Sphingobium japonicum UT26, an archetypal gamma-hexachlorocyclohexane-degrading bacterium (2011)
  • Author(s): Y. Nagata, S. Natsui, R. Endo, Y. Ohtsubo, N. Ichikawa, A. Ankai, A. Oguchi, S. Fukui, N. Fujita, and M. Tsuda
  • Journal Title: Enzyme Microb. Technol Volume: 49 Pages: 499-508
  • Peer Reviewed
• [Journal Article] The lin genes for gamma-hexachlorocyclohexane degradation in Sphingomonas sp. MM-1 proved to be dispersed across multiple plasmids (2011)
  • Author(s): M. Tabata, R. Endo, M. Ito, Y. Ohtsubo, A. Kumar, M. Tsuda, and Y. Nagata
  • Journal Title: Biosci. Biotechnol. Biochem Volume: 75 Pages: 466-472
  • Peer Reviewed
• [Presentation] プラスミド保持細菌のプラスミドに対する応答の比較 (2013)
  • Author(s): 高橋裕里香,3人,津田雅孝,野尻秀昭
  • Organizer: 日本農芸化学会2013年大会
  • Place of Presentation: 仙台
  • Year and Date: 20130324-27
• [Presentation] IncP-9群プラスミドNAH7の接合伝達開始配列の解析 (2013)
  • Author(s): 岸田康平,井上慧,宮崎亮,大坪嘉行,永田裕二,津田雅孝
  • Organizer: 第7回日本ゲノム微生物学会年会
  • Place of Presentation: 長浜
  • Year and Date: 20130308-10
• [Presentation] Multiple plasmids ofγ-hexachlorocylohexane-degrading bacterium Sphingomonas sp (2012)
  • Author(s): M. Tabata, S. Ohhata, Y. Ohtsubo, Y. Nagata, and M. Tsuda
  • Organizer: MM-1. International Plasmid Biology Conference 2012
  • Place of Presentation: Santander, Spain
  • Year and Date: 20120911-16
• [Presentation] Relationship between intergeneric conjugative transfer of plasmid NAH7 and nitrogen-related phosphotransferase system (2012)
  • Author(s): K. Inoue, R. Miyazaki, Y. Ohtsubo, Y. Nagata, and M. Tsuda
  • Organizer: International Plasmid Biology Conference 2012
  • Place of Presentation: Santander, Spain
  • Year and Date: 20120911-16
• [Presentation] 環境汚染物質分解能を担うプラスミドの水平伝達 (2012)
  • Author(s): 津田雅孝
  • Organizer: 日本農芸化学会2012年大会
  • Place of Presentation: 京都
  • Year and Date: 20120322-26
• [Presentation] ナフタレン分解プラスミドNAH7の接合伝達関連遺伝子群のsinglecellレベルでの発現解析 (2012)
  • Author(s): 井上慧,宮崎亮,大坪嘉行,永田裕二,津田雅孝
  • Organizer: 日本農芸化学会2012年大会
  • Place of Presentation: 京都
  • Year and Date: 20120322-26
• [Presentation] IncP-7群プラスミドの接合伝達宿主域を規定する因子の探索 (2012)
  • Author(s): 瀬谷学人,井上慧,大坪嘉行,永田裕二,津田雅孝
  • Organizer: 日本農芸化学会2012年大会
  • Place of Presentation: 京都
  • Year and Date: 20120322-26
• [Presentation] Nitrogen-related phosphotransferase system of Pseudomonas putida KT2440 exerts negative effect onconjugative acquisition ofnaphthalene-catabolic plasmid NAH7 (2011)
  • Author(s): K. Inoue, R. Miyazaki, Y. Ohtsubo, Y. Nagata, and M. Tsuda
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo
  • Year and Date: 20110906-10
• [Presentation] Structural analysis of plasmids for gamma-hexachlorocyclohexanedegradation in Sphingomonas sp (2011)
  • Author(s): M. Tabata, S. Ohhata, Y. Ohtsubo, Y. Nagata, and M. Tsuda
  • Organizer: MM-1. International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo
  • Year and Date: 20110906-10
• [Presentation] ナフタレン分解プラスミドNAH7の接合伝達制御に関与する受容菌のリン酸基転移系 (2011)
  • Author(s): 井上慧,大坪嘉行,永田裕二,津田雅孝
  • Organizer: ゲノム微生物学会ワークショップ
  • Place of Presentation: 仙台
  • Year and Date: 20110820-21
• [Book] Mobile catabolic genetic elements in pseudomonads. In: Nojiri, H., M. Tsuda, M. Fukuda, and Y. Kamagata (eds), Biodegradative Bacteria
  • Author(s): M. Tsuda, Y. Ohtsubo, and H. Yano
  • Publisher: Springer Verlag, Tokyo