Biochemical studies on longevity using multicellular microorganism

2012 Fiscal Year Final Research Report

• Project/Area Number: 23658283
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: University of Tsukuba
• Principal Investigator: FUKAMIZU Akiyoshi 筑波大学, 生命環境系, 教授 (60199172)
• Project Period (FY): 2011 – 2012
• Keywords: 線虫 / アルギニンメチル化反応 / 細胞機能

Research Abstract

Caenorhabditis elegans, about 1 mm-long round worms, is a model organism with genetic advantages and used for the studies on cell death and longevity. On the other hand, as the technologies such as in vitro protein synthesis and Mass spectrometry are innovatively advanced, it becomes important to understand the biochemical functions of proteins. In the present study, combined with genetics and biochemistry, we tried toexamine the reactions of protein arginine methylation related to longevity.

Research Products

• [Journal Article] Conserved SAMS function in regulating egg-laying in C. elegans. (2013)
  • Author(s): Tamiya, H., Hirota, K., Takahashi, Y., Daitoku, H., Kaneko, Y., Sakuta, G., Iizuka, K., Watanabe, S., Ishii, N., and Fukamizu A
  • Journal Title: J. Recept. Signal Transduc Volume: 33 Pages: 56-62
  • DOI: Doi:10.3109/10799893.2012.756896
  • Peer Reviewed
• [Journal Article] Enhanced histamine production through the induction of histidine decarboxylase expression by phorbol ester in Jurkat cells (2012)
  • Author(s): Nagashima Y., Kako K., Kim JD., and Fukamizu A.
  • Journal Title: Mol. Med. Rep. Volume: 6 Pages: 944-948
  • DOI: Doi:10.3892/mmr.2012.1049
  • Peer Reviewed
• [Journal Article] Production of free methylarginines via the proteasome and autophagy pathways in cultured cells (2011)
  • Author(s): Shirakawa, T., Kako, K., Shimada, T.,Nagashima, Y., Nakamura, A., Ishida, J., and Fukamizu, A.
  • Journal Title: Mol. Med. Rep. Volume: 4 Pages: 615-620
  • DOI: Doi:10.3892/mmr.2011.488
  • Peer Reviewed
• [Journal Article] Asymmetric Arginine Dimethylation Determines Life Span in C. elegans by Regulating Forkhead Transcription Factor DAF-16 (2011)
  • Author(s): Takahashi, Y., Daitoku, H., Hirota, K., Tamiya, H., Yokoyama, A., Kako, K., Nagashima, Y., Nakamura, A., Shimada, T., Watanabe, S., Yamagata, K., Yasuda, K., Ishii, N., and Fukamizu, A
  • Journal Title: Cell Metab Volume: 13 Pages: 505-516
  • DOI: Doi:10.1016/j.cmet.2011.03.017
  • Peer Reviewed
• [Presentation] Jurkat細胞におけるホルボールエステル刺激下でのヒスチジン脱炭酸酵素誘導性ヒスタ ミンの直接測定 (2012)
  • Author(s): 永島裕介、加香孝一郎、金 俊達、深水昭吉
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-15
• [Presentation] Regulation of S-adenosylmethionine synthetase-1 gene expression in the nematode Caenorhabditis elegans (2012)
  • Author(s): Sakuta, G., Takahashi, Y., Hirota, K., Daitoku, H. and Fukamizu, A
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-11
• [Presentation] 官能基に着目した妊娠高血圧マウス胎仔で増減するアミン類の探索法 (2012)
  • Author(s): 加香孝一郎、中村あゆみ、石田純治、永島裕介、嶋田崇史、深水昭吉
  • Organizer: 第16回活性アミンに関するワークショップ
  • Place of Presentation: 北海道医療大学
  • Year and Date: 2012-08-24
• [Presentation] The promoter region of sams-1 gene plays an important role in determining brood size regulation. (2012)
  • Author(s): Sakuta, G., Tamiya, H., Hirota, K., Takahashi, Y., Daitoku, H., Kaneko, Y., Iizuka,K., and Fukamizu, A
  • Organizer: 5thEast Asia C. elegans Meeting
  • Place of Presentation: Chientan Youth Activity Center、台湾
  • Year and Date: 2012-06-28
• [Presentation] Jurkat 細胞におけるホルボールエステル刺激下でのヒスチジン脱炭酸酵素誘導性ヒスタミンの直接測定 (2012)
  • Author(s): 永島裕介、加香孝一郎、金 俊達、深水昭吉
  • Organizer: 第7回日本ケミカルバイオロジー学会年会
  • Place of Presentation: 京都大学
  • Year and Date: 2012-06-08
• [Presentation] 線虫S-adenosylmethionine synthetase-1の産卵数制御機構の解析 (2011)
  • Author(s): 田宮寛子、廣田恵子、高橋悠太、大徳浩照、石井直明、深水昭吉
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-13
• [Presentation] S-adenosylmethionine synthetase SAMS-1 regurates brood size in C. elegans. (2011)
  • Author(s): Tamiya, H., Hirota, K., Takahashi, Y., Daitoku, H., Ishii, N., and Fukamizu, A
  • Organizer: 18thInternational C.elegans Meeting、University of California
  • Place of Presentation: アメリカ
  • Year and Date: 2011-06-22