2012 Fiscal Year Final Research Report
Analysis of the molecular basis for mRNA dynamics in the nucleus using next generation sequencer and the function for the factor(s)of quality control.
| Project/Area Number |
23658289
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Kyoto University |
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Principal Investigator |
MASUDA Seiji 京都大学, 大学院・生命科学研究科, 准教授 (20260614)
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| Co-Investigator(Renkei-kenkyūsha) |
OKUMURA Katsuzumi 三重大学, 大学院・生物資源学研究科, 教授 (30177183)
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| Project Period (FY) |
2011 – 2012
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| Keywords | mRNA / 次世代シークエンサ / 品質管理因子 |
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| Research Abstract |
The mRNA quality control in the nucleus remains largely unknown. Here, to analyze the molecular function of mRNA quality control factors in the nucleus, whole transcriptome analysis of the mRNA dynamics in the nucleus was exploited. And the importance of the mRNA quality control was also investigated using the conventional biochemical and molecular biology methods. The whole transcriptome was analyzed from the cells treated with siRNA of RRP45, a core component of exosome, or MTR4, a subcomponent of exosome. Whole reads were mapped on the human genome and the changes of the number of reads mapped on the specific region were compared between siRNA treated- and control cells. The genes whose expressions were markedly increased wereselected by the treatment of siRNA of RRP45 or MTR4. Next, the identification and localization of human TRAMP (PAPD5-ZCCHC7-MTR4) complex were analyzed using conventional methods like immunoprecipitation and immunostaining. Exosome and MTR4 were localized in the nucleolus. PAPD5 and ZCCHC7 were localized in the nucleus. The candidate molecules of human TRAMP complex were associated in the nuclear extract.
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Research Products
(21 results)
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[Journal Article] Establishment of the monitoring system which detects the inhibition of mRNA processing.2012
Author(s)
Fujita, K-I., Okamura, M., Nishimoto, S., Kurihara, T., Momma, K., Miyamae, Y., Kambe, T., Nagao, M., Narita, H. and Masuda, S.
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Journal Title
Biosci. Biotechnol. Biochem.
Volume: 76
Pages: 1248-1251
URL
Peer Reviewed
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[Journal Article] Enhancing recombinant protein production in human cell lines with a constitutive transport element and mRNA export proteins.2011
Author(s)
Aihara. Y., Fujiwara, N., Yamazaki, T., Kambe, T., Nagao, M., Hirose, Y., Masuda, S.
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Journal Title
J. Biotechnol.
Volume: 153
Pages: 86-91
DOI
Peer Reviewed
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[Journal Article] Constitutively active soluble form of erythropoietin receptor suppresses growth and angiogenesis of xenografts of transfected cancer cell lines.2011
Author(s)
Yasuda, Y., Maeda, Y., Hara, S., Tanaka, M., Koike, E., Watanabe, Y., Masuda, S., Yamasaki, H., Konishi, H., Horiuchi, Y. and Hoshiai, H.
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Journal Title
J. Cancer Therapy
Volume: 2
Pages: 40-53
URL
Peer Reviewed
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[Journal Article] Tissue nonspecific alkaline phosphatase is activated via a two-step mechanism by zinc transportcomplexes in the early secretory pathway.2011
Author(s)
Fukunaka, A., Kurokawa, Y., Teranishi, F., Sekler, I., Oda, K., Ackland, ML., Faundez, V., Hiromura, M., Masuda, S., Nagao, M., Enomoto, S. and Kambe, T.
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Journal Title
J Biol Chem.
Volume: 286
Pages: 16363-16373
DOI
Peer Reviewed
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