2012 Fiscal Year Final Research Report
Creation of a high efficient luciferase
| Project/Area Number |
23659021
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | Kyoto University |
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Principal Investigator |
NAKATSU Toru 京都大学, 大学院・薬学研究科, 准教授 (50293949)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 生物発光 |
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| Research Abstract |
The emission reaction is inhibited by the emission reaction by the product inhibition caused by the production of oxyluciferin in firefly luciferase. In order to understand the mechanism of the product inhibition, I determine the crystal structure of H247A mutant which decreases the degree of the product inhibition. The crystal structure shows that the interaction of between N- and C- terminal domain is weaken by the structural change. Because the active site might open easily , it is difficult to occur the product inhibition.
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