2012 Fiscal Year Final Research Report
The development in vivo siRNA delivery system for the treatment of renal fibrosis
Project/Area Number |
23659026
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Physical pharmacy
|
Research Institution | Jichi Medical University |
Principal Investigator |
|
Project Period (FY) |
2011 – 2012
|
Keywords | ドラッグデリバリー / 遺伝子治療 / 腎線維化 |
Research Abstract |
1) We detected that smad4-siRNA could significantly inhibit the epithelial mesenchymal transition of HK-2 cells with stimulation of TGF-β1. 2) We intravenously injected modified smad4-siRNA that was added extra bases at 5 prime of antisense strand and at 3 prime of sense strand to increase its stability in vivo (stable-smad4-siRNA) to renal fibrosismice. We detected t
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