2012 Fiscal Year Final Research Report
The development in vivo siRNA delivery system for the treatment of renal fibrosis
| Project/Area Number |
23659026
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | Jichi Medical University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | ドラッグデリバリー / 遺伝子治療 / 腎線維化 |
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| Research Abstract |
1) We detected that smad4-siRNA could significantly inhibit the epithelial mesenchymal transition of HK-2 cells with stimulation of TGF-β1.
2) We intravenously injected modified smad4-siRNA that was added extra bases at 5 prime of antisense strand and at 3 prime of sense strand to increase its stability in vivo (stable-smad4-siRNA) to renal fibrosismice. We detected t
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