2012 Fiscal Year Final Research Report
Exploratory research for the mechanisms underlying protective effects of bone marrow mesenchymal stem cells against brain damages
| Project/Area Number |
23659028
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Hokkaido University |
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Principal Investigator |
MINAMI Masabumi 北海道大学, 大学院・薬学研究院, 教授 (20243040)
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| Co-Investigator(Renkei-kenkyūsha) |
KATAYAMA Takahiro 北海道大学, 大学院・薬学研究院, 助教(2012年3月31日まで) (90399957)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 血液脳関門 / 骨髄幹細胞 / 脳実質浸潤 / セルマイグレーション / リアルタイムイ メージング |
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| Research Abstract |
To study transmigration of mesenchymal stem cells (MSCs) across the blood-brain barrier (BBB), we developed an in vitro BBB system consisting of rat brain microvascular endothelial cells (BMECs). Time-lapse imaging using this system revealed that MSCs transmigrated across the BMEC monolayer through transiently formed intercellular gaps between the BMECs. In addition, we developed an in vitro imaging system for simultaneous analysis in real time between claudin-5, a tight junction protein, and intracellular calcium dynamics in BMECs during MSC transmigration.
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