2012 Fiscal Year Final Research Report
Is the Lyn-ACSL3 system involved in induction of cell polarization ?
| Project/Area Number |
23659030
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biological pharmacy
|
|---|
| Research Institution | Chiba University |
|---|
Principal Investigator |
YAMAGUCHI Naoto 千葉大学, 大学院・薬学研究院, 教授 (00166620)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
FUKUMOTO Yasunori 千葉大学, 大学院・薬学研究院, 講師 (10447310)
NAKAYAMA Yuji 京都薬科大学, 薬学部, 教授 (10280918)
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Keywords | 細胞極性 / シグナル伝達 / 共焦点顕微鏡 / 細胞膜 / 蛋白質輸送 |
|---|
| Research Abstract |
To examine the localizations of c-Src and Lyn tyrosine kinases in polarized cells, we generated c-Src- or Lyn-inducible Madin-Darby canine kidney epithelial cell (MDCK II) lines. Using these inducible cell lines, we revealed that Lyn is largely localized to basolateral membranes but c-Src is present on both apical and basolateral membranes. In non-polarized cells, Lyn but not c-Src is accumulated in the Golgi region after biosynthesis in the cytoplasm and is transported to the plasma membrane through Lyn's association with long-chain acyl-CoA synthetase 3 (ACSL3) on Golgi membranes. Therefore, further elucidation of a role for the Lyn-ACSL3 system in cell polarization is needed.
|
