2012 Fiscal Year Final Research Report
Approach to reveal dynamics of caspase activation in maintainingbrain homeostasis
| Project/Area Number |
23659032
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 脳 / カスパーゼ / 神経免疫 |
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| Research Abstract |
Caspases are evolutionary conserved cystein protease. In this study, we tried to reveal dynamics of caspase activation in maintaining brain homeostasis. For this purpose, we utilized two novel probes that allow us to detect caspases-1 or caspase-3 in real-time. However, we have not detected weak or mild activation of those caspases in the brain derived from sleep-deprived mouse or in primary cell culture. These results suggest that further improvement in detection method will be needed to observe weak or mild activation of caspases by these probes.
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