2011 Fiscal Year Final Research Report
Establishment of YAP-dependent hepatic tumor formation and global expression analysis of related microRNAs
| Project/Area Number |
23659034
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
NISHINA Hiroshi 東京医科歯科大学, 難治疾患研究所, 教授 (60212122)
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| Project Period (FY) |
2010 – 2011
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| Keywords | YAP / 肝癌 / 細胞競合 / アポトーシス / cDNA / マイクロRNA / HTVi法 / 網羅的遺伝子解析 |
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| Research Abstract |
Recently, YAP was shown to play an important role in organ size control and to be inhibited by the Hippo signaling pathway. Either transgenic overexpression of YAP or knockout of Hippo pathway genes in mouse liver results in enlargement of this organ and the eventual development of hepatic tumors. In this study, we used the method of hydrodynamic tail vein injection(HTVi) that is specific for gene transfer into liver. As a results, we succeeded in establishing an efficient system of liver cancer induction due to gain of function of the active YAP. Secondly, we analyzed the gene expression by cDNA microarray and identified more than 20 genes whose expression is increased by active YAP. Thus, our results could contribute to elucidate the mechanism of human liver cancer
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[Journal Article] Stress-activated protein kinase MKK7 regulates axon elongation in the developing cerebral cortex2011
Author(s)
Tokiwa Yamasaki, Hiroshi Kawasaki, Satoko Arakawa, Kimiko Shimizu, Shigeomi Shimizu, Orly Reiner, Hideyuki Okano, Sachiko Nishina, Noriyuki Azuma, Josef M. Penninger, Toshiaki Katada and Hiroshi Nishina
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Journal Title
J. Neurosci
Volume: 31
Pages: 16872-16883
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[Journal Article] Autotaxin regulates vascular development via multiple lysophosphatidic acid(LPA) receptors in zebrafish2011
Author(s)
Hiroshi Yukiura, Kotaro Hama, Keita Nakanaga, Masayuki Tanaka, Yoichi Asaoka, Shinichi Okudaira, Naoaki Arima, Asuka Inoue, Takafumi Hashimoto, Hiroyuki Arai, Atsuo Kawahara, Hiroshi Nishina, and Junken Aoki
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Journal Title
J. Biol. Chem
Volume: 286
Pages: 43972-43983
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