2013 Fiscal Year Final Research Report
Identification of cysteine involved in oxidation-induced inhibition of channel function
| Project/Area Number |
23659037
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Kyoto University |
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Principal Investigator |
KAKIZAWA Sho 京都大学, 薬学研究科(研究院), 准教授 (40291059)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 酸化シグナル / 老化 / 翻訳後修飾 / チャネル |
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| Research Abstract |
Nitric-oxide-induced calcium release (NICR) in cerebellar Purkinje cells was revealed to be inhibited by oxidative signal and aging. Correspondingly, S-nitrosylation of calcium-release channels, essential for NICR, was also impaired by oxidative signal and aging. Biochemical analysis further confirmed that oxidation was induced in the channels by oxidative signal and aging. These observations indicate that oxidative signal inhibit S-nitrosylation as well as NICR through formation of disulfide bond in which cysteine residue of S-nitrosylation site is involved. Because the cysteine residue is incorporated into the disulfide bond, the residue is no longer susceptible to NO and S-nitrosylation.
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Research Products
(35 results)
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[Journal Article] TRIM50 regulates vesicular trafficking for acid secretion in gastric parietal cells2012
Author(s)
Nishi M, Aoyama F, Kisa F, Zhu H, Sun M, Lin P, Ohta H, Van B, Yamamoto S, Kakizawa S, Sakai H, Ma J, Sawaguchi A, Takeshima H
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Journal Title
J Biol Chem
Volume: 287
Pages: 33523-33532
Peer Reviewed
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[Journal Article] Nitric oxide-induced calcium release via ryanodine receptors regulates neuronal function2012
Author(s)
Kakizawa S, Yamazawa T, Chen Y, Ito A, Murayama T, Oyamada H, Kurebayashi N, Sato O, Watanabe M, Mori N, Oguchi K, Sakurai T, Takeshima H, Saito N & Iino M
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Journal Title
EMBO J
Volume: 31
Pages: 417-428
DOI
Peer Reviewed
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[Journal Article] TRIC-A Channels in Vascular Smooth Muscle Contribute to Blood Pressure Maintenance2011
Author(s)
Yamazaki D, Tabara Y, Kita S, Hanada H, Komazaki S, Naitou D, Mishima A, Nishi M, Yamamura H, Yamamoto S, Kakizawa S, Miyachi H, Yamamoto S, Miyata T, Kawano Y, Kamide K, Ogihara T, Hata A, Umemura S, Soma M, Takahashi N, Imaizumi Y, Miki T, Iwamoto T & Takeshima H
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Journal Title
Cell Metab
Volume: 14
Pages: 231-241
DOI
Peer Reviewed
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[Presentation] 一酸化窒素依存的カルシウム放出(NICR)はリアノジン受容体1 型を介し小脳シナプス可塑性に必須である2012
Author(s)
柿澤 昌, 山澤 徳志子, 村山 尚, 小山田 英人, 呉林 なごみ, 渡辺 雅彦, 森 望, 小口 勝司, 櫻井 隆, 竹島 浩, 飯野 正光
Organizer
第85回日本薬理学会年会
Place of Presentation
京都市
Year and Date
20120300
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[Presentation] リアノジン受容体を介した一酸化窒素によるカルシウム放出2012
Author(s)
山澤 徳志子, 柿澤 昌, 陳 毅力, 伊藤 明博, 村山 尚, 小山田 英人, 呉林なごみ, 佐藤 治, 櫻井 隆, 小口 勝司, 竹島 浩, 斉藤 延人, 飯野 正光
Organizer
第85回日本薬理学会年会
Place of Presentation
京都市
Year and Date
20120300
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[Presentation] リアノジン受容体を介したNO によるCa2+放出の機能的意義2011
Author(s)
山澤 徳志子, 柿澤 昌, 陳 毅力, 伊藤 明博, 村山 尚, 小山田 英人, 呉林なごみ, 佐藤 治, 櫻井 隆, 小口 勝司, 竹島 浩, 斉藤 延人, 飯野 正光
Organizer
第21回日本循環薬理学会
Place of Presentation
岡山市
Year and Date
20111200
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[Presentation] 一酸化窒素依存的Ca^<2+>放出(NICR)はリアノジン受容体を介する新規Ca^<2+>放出機構で小脳シナプス可塑性を誘導する.2011
Author(s)
柿澤 昌, 山澤 徳志子, 村山 尚, 小山田 英人, 呉林 なごみ, 渡辺 雅彦, 森 望, 小口勝司, 櫻井 隆, 竹島 浩, 飯野 正光
Organizer
第34回日本神経科学会大会
Place of Presentation
横浜市
Year and Date
20110900
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