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2012 Fiscal Year Final Research Report

Regulation mechanisms involved in tissue-specific gene expression

Research Project

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Project/Area Number 23659040
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biological pharmacy
Research InstitutionOsaka University

Principal Investigator

DOI Takefumi  大阪大学, 薬学研究科, 教授 (00211409)

Co-Investigator(Kenkyū-buntansha) OKADA Yoshiaki  大阪大学, 薬学研究科, 准教授 (50444500)
Project Period (FY) 2011 – 2012
Keywords組織特異的遺伝子発現 / Robo4遺伝子 / DNAメチル化 / エピジェネティクス
Research Abstract

Gene expression of the genomic DNA is tightly regulated. Some genes are specifically expressed in particular tissues, although it is not clearly understood how tissue-specific gene expression is regulated. Previous research on skeletal muscle has evidently shown that the skeletal muscle-specific transcription factor MyoD regulates skeletal muscle-specific genes. However, master regulators similar to MyoD have not been identified in most other tissues, suggesting that tissue-specific gene expression is regulated by other mechanisms as well. To investigate these putative mechanisms, we chose the endothelial cell (EC)-specific gene Robo4 and studied the regulation of its gene expression at the transcriptional level, which occurs via transcription factors and epigenetic controls.

  • Research Products

    (13 results)

All 2013 2012 2011 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (7 results) Remarks (1 results)

  • [Journal Article] An induced pluripotent stem cell-mediated and integration-free factor VIII expression system2013

    • Author(s)
      Yakura Y, Ishihara C, Kurosaki H, Kazuki Y, Komatsu N, Okada Y, Doi T, Takeya H, Oshimura M.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: Vol.431 Pages: 336-341

    • Peer Reviewed
  • [Journal Article] Transcriptional regulation of the endothelial cell-specific receptor, Robo42012

    • Author(s)
      Okada Y
    • Journal Title

      Yakugaku Zasshi

      Volume: Vol.132 Pages: 1045-1049

    • Peer Reviewed
  • [Journal Article] Embryonic stem cell differentiation system for evaluating gene functions involved in physiological megakaryocytic differentiation2012

    • Author(s)
      Okada Y, Yonekura M, Watanabe M, Nakai T, Wakimura A, Shimizu M, Kamikawa Y, Kitayama M, Kitajima K, Aird WC, Doi T
    • Journal Title

      Biochem Biophys Res Commun

      Volume: Vol.419 Pages: 477-481

    • Peer Reviewed
  • [Journal Article] Multiple ETS Family Proteins Regulate PF4 GeneExpression by Binding to the Same ETS Binding Site2011

    • Author(s)
      Okada Y, Nobori H, Shimizu M, Watanabe M, Yonekura M, Nakai T, Kamikawa Y, Wakimura A, Funahashi N, Naruse H, Watanabe A, Yamasaki D, Fukada S, Yasui K, Matsumoto K, Sato T, Kitajima K, Nakano T, Aird WC, Doi T
    • Journal Title

      PLoS One

      Volume: Vol.6 Pages: 1-10

    • Peer Reviewed
  • [Journal Article] New Protein Purification System Using Gold-Magnetic Beads and a Novel Peptide Tag, "the Methionine Tag"2011

    • Author(s)
      Okada Y, Takano TY, Kobayashi N, Hayashi A, Yonekura M, NishiyamaY, Abe T, Yoshida T, Yamamoto TA, Seino S, Doi T
    • Journal Title

      Bioconjug. Chem

      Volume: Vol.22 Pages: 887-893

    • Peer Reviewed
  • [Presentation] Robo4 遺伝子の組織特異的な発現はDNA メチル化により制御される-血管内皮細胞特異的な遺伝子発現が生み出されるメカニズムとは-2012

    • Author(s)
      岡田欣晃, William Aird, 土井健史
    • Organizer
      第62回日本薬学会近畿支部総会・大会
    • Place of Presentation
      武庫川女子大学薬学部(兵庫県)(招待講演)
    • Year and Date
      2012-10-20
  • [Presentation] Robo4遺伝子の組織特異的な発現を制御するDNA メチル化サイトについての解析2012

    • Author(s)
      西山侑児、酒井美貴、鈴木綾乃、加納義浩、舟橋伸昭、岡田欣晃、土井健史
    • Organizer
      第13回Pharmaco-Hematologyシンポジウム
    • Place of Presentation
      日本薬学会長井記念ホール(東京都)
    • Year and Date
      2012-06-16
  • [Presentation] DNA メチル化サイトへの点変異導入はRobo4プロモーターの組織特異性を失わせる2012

    • Author(s)
      岡田欣晃、西山侑児、鈴木綾乃、酒井美貴、舟橋伸昭、加納義浩、William Aird、土井健史
    • Place of Presentation
      学術総合センター(東京都)
    • Year and Date
      2012-05-14
  • [Presentation] 血管内皮細胞特異的遺伝子発現の制御メカニズム解析2012

    • Author(s)
      岡田欣晃
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)(招待講演)
    • Year and Date
      2012-03-28
  • [Presentation] Robo4プロモーターの組織特異的なDNAメチル化パターンが決定されるメカニズムの解析2012

    • Author(s)
      酒井美貴、西山侑児、舟橋伸昭、柿内康司、間庭佑典、岡田欣晃、土井健史
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2012-03-28
  • [Presentation] DNA メチル化によるRobo4遺伝子の組織特異的発現の制御 Robo4 遺伝子はなぜ血管内皮細胞特異的に発現するのか-2011

    • Author(s)
      岡田欣晃、西山侑児、鈴木綾乃、酒井美貴、舟橋伸昭、加納義浩、成瀬啓樹、William Aird、土井健史
    • Organizer
      第61回日本薬学会近畿支部総会・大会
    • Place of Presentation
      神戸学院大学 ポートアイランドキャンパス(兵庫県)
    • Year and Date
      2011-10-22
  • [Presentation] DNAメチル化はRobo4遺伝子を血管内皮細胞特異的に発現させる2011

    • Author(s)
      岡田欣晃、舟橋伸昭、加納義浩、西山侑児、成瀬啓樹、鈴木綾乃、William Aird、土井健史
    • Organizer
      第5回日本エピジェネティクス研究会年会
    • Place of Presentation
      KKRホテル熊本(熊本県)
    • Year and Date
      2011-05-19
  • [Remarks]

    • URL

      http://www.phs.osaka-u.ac.jp/homepage/b018/index.html

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Published: 2014-09-25  

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