2012 Fiscal Year Final Research Report
Regulation mechanisms involved in tissue-specific gene expression
| Project/Area Number |
23659040
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biological pharmacy
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
DOI Takefumi 大阪大学, 薬学研究科, 教授 (00211409)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OKADA Yoshiaki 大阪大学, 薬学研究科, 准教授 (50444500)
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Keywords | 組織特異的遺伝子発現 / Robo4遺伝子 / DNAメチル化 / エピジェネティクス |
|---|
| Research Abstract |
Gene expression of the genomic DNA is tightly regulated. Some genes are specifically expressed in particular tissues, although it is not clearly understood how tissue-specific gene expression is regulated. Previous research on skeletal muscle has evidently shown that the skeletal muscle-specific transcription factor MyoD regulates skeletal muscle-specific genes. However, master regulators similar to MyoD have not been identified in most other tissues, suggesting that tissue-specific gene expression is regulated by other mechanisms as well. To investigate these putative mechanisms, we chose the endothelial cell (EC)-specific gene Robo4 and studied the regulation of its gene expression at the transcriptional level, which occurs via transcription factors and epigenetic controls.
|
-
-
-
[Journal Article] Embryonic stem cell differentiation system for evaluating gene functions involved in physiological megakaryocytic differentiation2012
Author(s)
Okada Y, Yonekura M, Watanabe M, Nakai T, Wakimura A, Shimizu M, Kamikawa Y, Kitayama M, Kitajima K, Aird WC, Doi T
-
Journal Title
Biochem Biophys Res Commun
Volume: Vol.419
Pages: 477-481
Peer Reviewed
-
[Journal Article] Multiple ETS Family Proteins Regulate PF4 GeneExpression by Binding to the Same ETS Binding Site2011
Author(s)
Okada Y, Nobori H, Shimizu M, Watanabe M, Yonekura M, Nakai T, Kamikawa Y, Wakimura A, Funahashi N, Naruse H, Watanabe A, Yamasaki D, Fukada S, Yasui K, Matsumoto K, Sato T, Kitajima K, Nakano T, Aird WC, Doi T
-
Journal Title
PLoS One
Volume: Vol.6
Pages: 1-10
Peer Reviewed
-
[Journal Article] New Protein Purification System Using Gold-Magnetic Beads and a Novel Peptide Tag, "the Methionine Tag"2011
Author(s)
Okada Y, Takano TY, Kobayashi N, Hayashi A, Yonekura M, NishiyamaY, Abe T, Yoshida T, Yamamoto TA, Seino S, Doi T
-
Journal Title
Bioconjug. Chem
Volume: Vol.22
Pages: 887-893
Peer Reviewed
-
-
-
-
-
-
-
-
