2012 Fiscal Year Final Research Report
Exploration of a system for cellular response to the electrophilesexhibiting skin sensitization potential
| Project/Area Number |
23659063
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Environmental pharmacy
|
|---|
| Research Institution | University of Tsukuba |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SHINKAI Yasuhiro 筑波大学, 医学医療系, 助教 (10454240)
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Keywords | 化学物質過敏症 / 3,4-ジヒドロクマリン / アミノ基 / 共有結合 |
|---|
| Research Abstract |
3,4-dihydrocoumarin (3,4-DHC) is known to be a potent skin sensitizer that has electrophilic property to from a covalent bond with deprotonated amino groups. Incubation of 3,4-DHC in aqueous solution resulted in hydrolysis to form 3-(2-hydroxyphenyl)propanoic acid (HPA) and formation of 3,4-DHC-lysine adduct in the presence of lysine. Also, LC-MS analysis showed that 3,4-DHC modified lysine residues in KLH. By using such a 3,4-DHC-KLH adduct, we prepared the polyclonal antibody against 3,4-DHC.
|
