2013 Fiscal Year Final Research Report
New entry pathway of highly pathogenic avian influenza virus without sialic acid .
Project/Area Number |
23659069
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Environmental pharmacy
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Research Institution | Tokyo Metropolitan Institute of Medical Science |
Principal Investigator |
SAKURAI Akira 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 研究員 (40546628)
|
Project Period (FY) |
2011 – 2012
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Keywords | 皓病原性トリインフルエンザウイルス / インフルエンザ / 病原性 |
Research Abstract |
Highly pathogenic avian influenza (HPAI) H5N1 virus was characterized by the high pathogenicity and unique organ tropism for infected avian and mammalian. Here we show a new internalized pathway of HPAI H5N1 virus without 2-3 linked sialic acids on the cell surface. We revealed that HPAI H5N1 virus but not low pathogenic avian influenza (LPAI) H5N1 virus could infect in cells treated with neuraminidase for removing sialic acid on the cell surface, and the infected cells generated viral particles with intact infectivity. We then identified the multi-basic sequence (MBS) of HPAI H5N1 as a kind of cell penetrating peptides (CPPs) which is known to have an ability of penetrating cellular membrane. GFP fused with MBS could internalize into several kinds of cells, and specific antibody against MBS prevented the fusion protein from internalizing.
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[Journal Article] Broad-Spectrum Detection of H5 Subtype Influenza A Viruses with a New Fluorescent Immunochromatography System2013
Author(s)
Akira Sakurai, Katsuyoshi Takayama, Namiko Nomura, Tsubasa Munakata, Naoki Yamamoto, Tsuruki Tamura, Jitsuho Yamada, Masako Hashimoto, Kazuhiko Kuwahara, Yoshihiro Sakoda, Yoshihiko Suda, Yukuharu Kobayashi, Nobuo Sakaguchi, Hiroshi Kida, Michinori Kohara, Futoshi Shibasak
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Journal Title
PLoS one
Volume: 8 (11)
Pages: e76753
Peer Reviewed
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[Journal Article] Rapid typing of influenza viruses using super high-speed quantitative real-time PCR2011
Author(s)
Sakurai A, Nomura N, Nanba R, Sinkai T, Iwaki T, Obayashi T, Hashimoto K, Hasegawa M, Sakoda Y, Naito A, Morizane Y, Hosaka M, Tsuboi K, Kida H, Kai A, Shibasaki F
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Journal Title
J Virol Methods
Volume: Dec;178(1-2)
Pages: 75-81
Peer Reviewed
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