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2012 Fiscal Year Final Research Report

Are protein expression levels changed by intron SNPs on CYP2D6?

Research Project

  • PDF
Project/Area Number 23659070
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Medical pharmacy
Research InstitutionTohoku University

Principal Investigator

HIRATSUKA Masahiro  東北大学, 大学院・薬学研究科, 准教授 (50282140)

Co-Investigator(Renkei-kenkyūsha) TAKAHASHI Aki  東北大学, 大学院・薬学研究科, 助手 (30545695)
Project Period (FY) 2011 – 2012
Keywords薬物動態 / 代謝学
Research Abstract

We examined changes in the protein expression of the drug-metabolizing enzyme CYP2D6 by using an assay capable of analyzing the influence of intron SNPs, to determine the individual differences in protein activity that cannot be explained by exon SNPs alone. As a result, we found that while the positive control CYP2D6*41 showed a 35% decrease in protein expression compared to the wild-type CYP2D6*1, none of the other 4 variants differed greatly from the wild-type.

  • Research Products

    (7 results)

All 2012 2011

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (2 results)

  • [Journal Article] In vitro assessment of the allelic variants of cytochrome P4502012

    • Author(s)
      Masahiro Hiratsuka
    • Journal Title

      Drug Metab. Pharmacokinet.

      Volume: 27 Pages: 68-84

    • URL

      http://www.ncbi.nlm.nih.gov/pu bmed/22041138

    • Peer Reviewed
  • [Journal Article] Ilse van der Heiden, Ron HN van Schaik, Jan Jakobsson, Magnus Ingelman-Sundberg. Influence of sex on propofol metabolism, a pilot study: implications for propofol anesthesia2012

    • Author(s)
      Irena Loryan, Marja Lindqvist, Inger Johansson, Masahiro Hiratsuka
    • Journal Title

      Eur. J. Clin. Pharmacol

      Volume: 68 Pages: 397-406

    • DOI

      DOI=10.1007/s00228-011-1132-2

    • Peer Reviewed
  • [Journal Article] Functional characterization of CYP2B6 allelic variants in demethylation of anti-malarial artemether2011

    • Author(s)
      Masashi Honda, Yuka Muroi, Yuichiro Tamaki, Daisuke Saigusa, Naoto Suzuki, Yoshihisa Tomioka, Yoichi Matsubara, Akifumi Oda, Noriyasu Hirasawa, Masahiro Hiratsuka
    • Journal Title

      Drug Metab. Dispos

      Volume: 39 Pages: 1860-1865

    • DOI

      DOI=10.1124/dmd.111.040352

    • Peer Reviewed
  • [Journal Article] Association between cancer risk and drug metabolizing enzyme gene (CYP2A6, CYP2A13, CYP4B1, SULT1A1, GSTM1, and GSTT1) polymorphisms in Japanese cases of lung cancer2011

    • Author(s)
      Yuichiro Tamaki, Tomio Arai, Haruhiko Sugimura, Takamitsu Sasaki, Masashi Honda, Yuka Muroi, Yoichi Matsubara, Shuichi Kanno, Masaaki Ishikawa, Noriyasu Hirasawa, Masahiro Hiratsuka
    • Journal Title

      Drug Metab. Pharmacokinet

      Volume: 26 Pages: 516-522

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/21791872

    • Peer Reviewed
  • [Journal Article] Novel single nucleotide polymorphism of CYP2A13 gene in a Japanese population2011

    • Author(s)
      Yuichiro Tamaki, Masashi Honda, Yuka Muroi, Tomio Arai, Haruhiko Sugimura, Yoichi Matsubara, Shuichi Kanno, Masaaki Ishikawa, Noriyasu Hirasawa, Masahiro Hiratsuka
    • Journal Title

      Drug Metab. Pharmacokinet.

      Volume: 26 Pages: 544-547

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/21606606

    • Peer Reviewed
  • [Presentation] ファーマコゲノミクス解析による個別化薬物療法の推進2012

    • Author(s)
      平塚真弘
    • Organizer
      第22回日本医療薬学会年会
    • Place of Presentation
      新潟
    • Year and Date
      2012-10-28
  • [Presentation] 遺伝子多型情報に基づく薬物代謝酵素バリアントの酵素反応速度論的機能解析2011

    • Author(s)
      平塚真弘
    • Organizer
      日本薬学会東北支部第10回医療系薬学若手研究者セミナー
    • Place of Presentation
      東北大学
    • Year and Date
      2011-09-17

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Published: 2014-09-25  

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