2012 Fiscal Year Final Research Report
Development of a novel nucleic acid drug targeting sense/antisense transcript pairs
| Project/Area Number |
23659080
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Medical pharmacy
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
OBIKA Satoshi (80243252)
|
|---|
| Research Collaborator |
MIZUGUCHI Hiroyuki 大阪大学, 大学院・薬学研究科, 教授
TACHIBANA Masashi 大阪大学, 大学院・薬学研究科, 助教
KATAYAMA Kazufumi 大阪大学, 大学院・薬学研究科, 助教
GOTO Hitoshi 大阪大学, 大学院・薬学研究科, 大学院生
NANJO Yuki 大阪大学, 大学院・薬学研究科, 大学院生
TANINO Fumihito 大阪大学, 大学院・薬学研究科, 大学院生
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Keywords | 非コード RNA / 核酸医薬 / 遺伝子発現制御 / がん / 非翻訳領域 / RNA activation |
|---|
| Research Abstract |
Recent studies have demonstrated that small duplex RNA complementary to the promoter region of target gene enhances expression of target genes. This phenomenon is designated as “RNA activation”. In this study, we examined whether expression of the matrix metalloproteinase (MMP) inhibitor RECK is activated in the cultured tumor cell lines by transfection with small duplex RNA (dsRNA) complementary to the promoter of RECK gene, leading to the suppression of expression of MMPs and invasion of the tumor cells. Transfection with the dsRNAs significantly elevated RECK expression levels by approximately 6-fold in human tumor cells. Furthermore, the dsRNA-induced elevation of RECK expression resulted in reduction in expression of MMPs and invasion of tumor cells. These results suggest that small duplex RNA complementary to the promoter region of tumor suppressor genes would have the potential as a novel antitumor agent.
|
