2012 Fiscal Year Final Research Report
Induction of spermatogenic cells from human multipotent stem cell by microinjection into the mouse testis
| Project/Area Number |
23659090
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Chiba University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ITO Chizuru 千葉大学, 大学院・医学研究院, 講師 (80347054)
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| Co-Investigator(Renkei-kenkyūsha) |
MAEKAWA Mamiko 千葉大学, 大学院・医学研究院, 助教 (20181571)
YAMATOYA Kenji 千葉大学, 大学院・医学研究院, 特任研究員 (80447309)
KAMIMURA Kyoko 千葉大学, 大学院・医学研究院, 技術専門職員 (20422264)
MUTOH Tohru 千葉大学, 大学院・医学研究院, 技術専門職員 (30422265)
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| Project Period (FY) |
2011 – 2012
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| Keywords | Muse細胞 / 精巣内移植 / 精子幹細胞 / 精子形成 |
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| Research Abstract |
EGFP-tagged Muse cells from human mesenchymal cells were prepared by established method. Thus prepared EGFP-Muse cells were microinjected into the testes (testicular seminiferous tubules) of W/Wv mutant male mice lacking spermatogenic stem cells, and we found the following results. Enough amount of live Muse cells were obtained even after long time journey for several hours. Muse cells prepared caused cell-cell aggregation when they were placed in the conditioned medium containing albumin for microinjection, but the microinjection efficiency rate increased when they were microinjected using medium lacking albumin. However, 3 month later, EGFP-Muse cell signal was not recognized at all in the injected testes, indicating that Muse cells in the transplanted medium in this study could not develop to the spermatogenic cells.
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Research Products
(49 results)
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[Journal Article] Galnt3 deficiency disrupts acrosome formation and leads to oligoasthenoteratozoospermia2013
Author(s)
Miyazaki T, Mori M, Yoshida CA, Ito C,Yamatoya K, Moriishi T, Kawai Y, Komori H, Kawane T, Izumi S, Toshimori K, Komori T
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Journal Title
Histochem and Cell Biol
Volume: 139(2)
Pages: 339-354
DOI
Peer Reviewed
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[Journal Article] Lipid rafts enriched in monosialylGb5Cer carrying the stage-specific embryonic antigen-4 epitope are involved in development of mouse preimplantation embryos at cleavage stage2011
Author(s)
Sato B, Katagiri YU, Miyado K, Okino N, Ito M, Akutsu H, Okita H, Umezawa A, Fujimoto J, Toshimori K, Kiyokawa N.
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Journal Title
BMC Dev Biol
Volume: 11:22
DOI
Peer Reviewed
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[Journal Article] GalNAcβ 1,3-linked paragloboside carries the epitope of a sperm maturation-related glycoprotein that is recognized by the monoclonal antibody MC121.2011
Author(s)
Katagiri YU, Sato B, Yamatoya K, Taki T, Goto-Inoue N, Setou M, Okita H, Fujimoto J, Ito C, Toshimori K, Kiyokawa N.
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Journal Title
Biochem Biophys Res Commun
Volume: 406(3)
Pages: 326-331
DOI
Peer Reviewed
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[Journal Article] Tysnd1 deficiency in mice interferes with the peroxisomal location of PTS2 enzymes, causinglipid metabolic abnormalities and maleinfertility
Author(s)
Mizuno Y, Ninomiya Y, Nakachi Y, Iseki M, Iwasa H, Akita M, Tsukui T, Shimozawa N, Ito C, Toshimori K, Nishimura M, Hara H, Maeba R, Okazaki T, Alodaib AN, Amoudi MA, Jacob M, Alkuraya FS, Horai Y, Watanabe M, Motegi H, Wakana S, Noda T, Kurochkin IV, Miz
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Journal Title
PLoS genet
Volume: 9(2)
Pages: e1003286.2013
DOI
Peer Reviewed
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[Presentation] Tysnd1 deficiency interferes with mouse peroxisomal functions2011
Author(s)
Mizuno Y, Ninomiya Y, Iseki M, Iwasa H, Akita M, Tsukui T, Ito C, Toshimori K,Shimozawa N, Nishimukai M, Hara H, MaebaR, Okazaki T, Horai Y, Watanabe M, Motegi H, Wakana S, Noda T, Kurochkin Igor V, Mizuno Y, Schoenbach C, Okazaki Y.
Organizer
第34回日本分子生物学会年会
Place of Presentation
パシフィコ横浜(神奈川県)(口演)
Year and Date
2011-12-15
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[Remarks] (3)VBコンペ受賞者決定日刊工業新聞2012年4月11日報道内容URL(2)報道関連情報
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[Remarks] (1)年森清隆なのはなコンペ2012ベンチャー志向先端研究部門なのはな賞(教員版)受賞2012年4月9日(3)受賞歴
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[Remarks] (2)年森清隆なのはなコンペ2012ベンチャー志向先端研究部門双葉電子記念財団助成金ちばぎんひまわり賞受賞2012年4月9日(3)受賞歴
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