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2012 Fiscal Year Final Research Report

Research on the mechanisms underlying developmental synaptogenesis using functional brainbow strategy

Research Project

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Project/Area Number 23659105
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General physiology
Research InstitutionTohoku University

Principal Investigator

YAWO Hiromu  東北大学, 大学院・生命科学研究科, 教授 (00144353)

Project Period (FY) 2011 – 2012
Keywords発生 / 分化 / シナプス形成 / トロフィック物質 / 自律神経節
Research Abstract

The calyx-type synapse of chick ciliary ganglion (CG) has been intensively studied for decades as a model system for the synaptic development, morphology and physiology. Here, we evaluated a method for manipulating the molecular and functional organization of the presynaptic terminals of this model synapse.

  • Research Products

    (44 results)

All 2013 2012 2011 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (24 results) Book (2 results) Remarks (4 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Optogenetic manipulation of neural and non-neural functions2013

    • Author(s)
      Yawo H, Asano T, Sakai S, Ishizuka T
    • Journal Title

      Dev Growth Differ

      Volume: 55(4) Pages: 474-490

    • DOI

      doi:10.1111/dgd.12053

    • Peer Reviewed
  • [Journal Article] Optogenetic probing and manipulation of the calyx-type presynaptic terminal in the embryonic chick ciliary ganglion2013

    • Author(s)
      Egawa R, Hososhima S, Hou X, Katow H, Ishizuka T, Nakamura H, Yawo H
    • Journal Title

      PLoS ONE

      Volume: 8(3) Pages: e59179

    • DOI

      DOI:10.1371/journal.pone.0059179

    • Peer Reviewed
  • [Journal Article] Adventure beyond borders of scientific fields with optogenetics2013

    • Author(s)
      Yawo H, Koizumi A, Hegemann P
    • Journal Title

      Neurosci Res

      Volume: 75 Pages: 1-2

    • DOI

      doi:10.1016/j.neures.2013.02.001

    • Peer Reviewed
  • [Journal Article] Channelrhodopsins - their potential in gene therapy for neurological disorders2013

    • Author(s)
      Ji Z-G, Ishizuka T, Yawo H
    • Journal Title

      Neurosci Res

      Volume: 75 Pages: 6-12

    • DOI

      doi:10.1016/j.neures.2012.09.004

    • Peer Reviewed
  • [Journal Article] Targeted expression of a chimeric channelrhodopsin in zebrafish under regulation of Gal4-UAS system2013

    • Author(s)
      Umeda K, Shoji W, Sakai S, Muto A, Kawakami K, Ishizuka T, Yawo H
    • Journal Title

      Neurosci Res

      Volume: 75(1) Pages: 69-75

    • DOI

      doi:10.1016/j.neures.2012.08.010

    • Peer Reviewed
  • [Journal Article] Involvement of glutamate 97 in ion influx through photo-activated channelrhodopsin-22013

    • Author(s)
      Tanimoto S, Sugiyama Y, Takahashi T, Ishizuka T, Yawo H
    • Journal Title

      Neurosci Res

      Volume: 75(1) Pages: 13-22

    • DOI

      doi:10.1016/j.neures.2012.05.008

    • Peer Reviewed
  • [Journal Article] Parallel and patterned optogenetic manipulation of neurons in the brain slice using a DMD-based projector2013

    • Author(s)
      Sakai S, Ueno K, Ishizuka T, Yawo H
    • Journal Title

      Neurosci Res

      Volume: 75(1) Pages: 59-64

    • DOI

      doi:10.1016/j.neures.2012.03.009

    • Peer Reviewed
  • [Journal Article] Remodeling of hippocampal network in pilocarpine-treated mice expressing synaptopHluorin in the mossy fiber terminals2012

    • Author(s)
      Ito S, Ishizuka T, Yawo H
    • Journal Title

      Neurosci Res

      Volume: 74(1) Pages: 25-31

    • DOI

      doi:10.1016/j.neures.2012.07.003

    • Peer Reviewed
  • [Journal Article] Light-evoked somatosensory perception of transgenic rats which express channelrhodopsin-2 in dorsal root ganglion cells2012

    • Author(s)
      Ji Z-G, Ito S, Honjoh T, Ohta H, Ishizuka T, Fukazawa Y, Yawo H
    • Journal Title

      PLoS ONE

      Volume: 7 Pages: e32699

    • DOI

      doi:10.1371/journal.pone.0032699

    • Peer Reviewed
  • [Journal Article] Light-evoked somatosensory perception of transgenic rats that express channelrhodopsin-2 in dorsal root ganglion cells2012

    • Author(s)
      Ji ZG, Ito S, Honjoh T, Ohta H, Ishizuka T, Fukazawa Y, Yawo H
    • Journal Title

      PLoS One

      Volume: 7(3) Pages: e32699

    • DOI

      doi:10.1371/journal.pone.0032699

    • Peer Reviewed
  • [Journal Article] Optically controlled contraction of photosensitive skeletal muscle cells2012

    • Author(s)
      Asano T, Ishizua T, Yawo H
    • Journal Title

      Biotechnol Bioeng

      Volume: 109(1) Pages: 199-204

    • DOI

      doi:10.1002/bit.23285

    • Peer Reviewed
  • [Journal Article] Evaluation of a Sindbis virus vector displaying an immunoglobulin-binding domain: antibody-dependent infection of neurons in living mice2011

    • Author(s)
      Konno A, Honjo T, Uchida A, Ishizuka T, Yawo H
    • Journal Title

      Neurosci Res

      Volume: 71(4) Pages: 328-334

    • DOI

      doi:10.1016/j.neures.2011.08.013

    • Peer Reviewed
  • [Presentation] Optogenetic manipulation of presynaptic activity in chick ciliary ganglion during synapse elimination2013

    • Author(s)
      Shoko Hososhima, Toru Ishizuka, Hiromu Yawo
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京
    • Year and Date
      20130327-29
  • [Presentation] An experimental platform to study physiology, morphology and development of the vertebrate presynaptic terminal with genetic manipulations2013

    • Author(s)
      Ryo Egawa, Shoko Hososhima, Saki Tanimoto, Toru Ishizuka, Harukazu Nakamura, Hiromu Yawo
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京
    • Year and Date
      20130327-29
  • [Presentation] Optogenetic manipulation of presynaptic activity in the ciliary ganglion of chick embryo2012

    • Author(s)
      Shoko Hososhima, Toru Ishizuka, Hiromu Yawo
    • Organizer
      7th International chick meeting
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      20121114-18
  • [Presentation] チャネルロドプシンを発現した骨格筋管細胞における筋収縮と成熟の光操作2012

    • Author(s)
      浅野豪文,石塚徹,八尾寛
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋
    • Year and Date
      20120922-24
  • [Presentation] An experimental platform for the study of presynaptic development, morphology and physiology - gene manipulation of the vertebrate model synapse, Cold Spring Harbor Laboratory Meeting2012

    • Author(s)
      Egawa R, Hososhima S, Tanimoto S, Ishizuka T, Nakamura H, Yawo H
    • Organizer
      Axon Guidance, Synapse Formation and Regeneration"
    • Place of Presentation
      Cold Spring Harbor, USA
    • Year and Date
      20120918-22
  • [Presentation] ニワトリ胚毛様体神経節における杯状シナプスの発達プロセス-Brainbow法による解析2012

    • Author(s)
      Egawa,R.,Hososhima,S.,Ishizuka,T.,Nakamura,H.,andYawo,H
    • Organizer
      東北脳科学ウィンタースクール
    • Place of Presentation
      仙台・蔵王
    • Year and Date
      20120218-19
  • [Presentation] 光操作システムの開発とオプトジェネティクス(光遺伝学)への応用2012

    • Author(s)
      八尾寛
    • Organizer
      第32回東邦大学生命科学シンポジウム
    • Place of Presentation
      船橋市
    • Year and Date
      2012-10-31
  • [Presentation] 杯状シナプスにおけるシナプス除去機構への光遺伝学的アプローチ2012

    • Author(s)
      細島頌子、石塚徹、八尾寛
    • Organizer
      第44回東北生理談話会
    • Place of Presentation
      山形
    • Year and Date
      2012-10-27
  • [Presentation] 光受容体チャネル、チャネルロドプシンのイオン流はGd3+によりブロックされる2012

    • Author(s)
      谷本早希、石塚徹、八尾寛
    • Organizer
      第44回東北生理談話会
    • Place of Presentation
      山形
    • Year and Date
      2012-10-27
  • [Presentation] 次世代シナプスモデルを用いた発達期シナプス刈込機構の研究2012

    • Author(s)
      江川遼、八尾寛
    • Organizer
      東北大学脳科学GCOE異分野融合研究報告会
    • Place of Presentation
      仙台
    • Year and Date
      2012-03-02
  • [Presentation] ニワトリ胚毛様体 神経節における杯状シナプスの形成- Brainbow 法による解析2011

    • Author(s)
      Egawa R, Hososhima S, Ishizuka T, Nakamura H, Yawo H
    • Organizer
      The 34th annualMeeting of the Japan Neuroscience Society (NEURO2011)
    • Place of Presentation
      横浜
    • Year and Date
      20110914-17
  • [Presentation] Synaptogenesis in the embryonic chick ciliary ganglion - a brainbow study. OIST International Workshop2011

    • Author(s)
      Egawa R, Hososhima S, Ishizuka T, Nakamura H, Yawo H
    • Organizer
      Molecular & structural organization of presynaptic function and plasticity
    • Place of Presentation
      Okinawa
    • Year and Date
      20110907-09
  • [Presentation] Development of the calyx-type synapses in the embryonic chick ciliary ganglion - a brainbow study2011

    • Author(s)
      Egawa R, Hososhima S, Ishizuka T, Nakamura H, Yawo H
    • Organizer
      8th IBRO WORLD CONGRESS OFNEUROSCIENCE
    • Place of Presentation
      Florence, Italy
    • Year and Date
      20110714-18
  • [Presentation] オプトジェネティクスを用いた培養骨格筋の光操作2011

    • Author(s)
      浅野豪文、石塚徹、八尾寛
    • Organizer
      生体情報インターフェース創生のためのフォトニクス研究‐共同プロジェクト研究会
    • Place of Presentation
      仙台
    • Year and Date
      2011-12-13
  • [Presentation] ニワトリ胚毛 様体神経節における杯状シナプスの発 達プロセス-Brainbow 法による解析2011

    • Author(s)
      Egawa, R., Hososhima, S., Ishizuka, T., Nakamura, H., and Yawo, H
    • Organizer
      生 化学若い研究者の会 若手シンポジウ ム
    • Place of Presentation
      仙台
    • Year and Date
      2011-12-10
  • [Presentation] Distribution of the actin-binding protein, coactosin in the chick ciliary ganglion during embryonic synaptogenesis2011

    • Author(s)
      細島頌子、侯旭濱、仲村春和、石塚徹、八尾寛
    • Organizer
      3rd UCL-Tohoku symposium
    • Place of Presentation
      London, UK
    • Year and Date
      2011-10-19
  • [Presentation] チャネルロドプシンを用いた光刺激による筋収縮制御2011

    • Author(s)
      浅野豪文、石塚徹、八尾寛
    • Organizer
      第1回オプトジェネティクス講習会
    • Place of Presentation
      仙台
    • Year and Date
      2011-09-23
  • [Presentation] Resonant rhythms generated by neurons and network- opto-current-clamp with optimizedchannelrhodopsins2011

    • Author(s)
      Yawo H, Wen L, Sakai S, Wang H, Tanimoto S, Egawa R, Ishizuka T
    • Organizer
      第84回日本生化学会 大会
    • Place of Presentation
      京都
    • Year and Date
      2011-09-21
  • [Presentation] Expression of the actin-bindingproteins, coactosin and drebrin, in the developing chick ciliary ganglion2011

    • Author(s)
      細島頌子、侯旭濱、仲村春和、石塚徹、八尾寛
    • Organizer
      第34回 日本神経科学大会
    • Place of Presentation
      横浜
    • Year and Date
      2011-09-17
  • [Presentation] The molecular determinants involved in ion flux regulation of channelrhodopsin-22011

    • Author(s)
      Tanimoto S, Wang H, Sugiyama Y, Ishizuka T, Yawo H
    • Organizer
      第34回日本神経科 学大会
    • Place of Presentation
      横浜
    • Year and Date
      2011-09-17
  • [Presentation] The molecular determinants involved in ion flux regulation of channelrhodopsins2011

    • Author(s)
      Tanimoto S, Wang H, Sugiyama Y, Ishizuka T, Yawo H
    • Organizer
      8th IBRO World Congress of Neuroscience
    • Place of Presentation
      Florence, Itary
    • Year and Date
      2011-07-17
  • [Presentation] 新世代チャネルロドプシンによるオプトジェネティクス.神経組織の成長・再生・移植研究会2011

    • Author(s)
      八尾寛,石塚徹
    • Organizer
      第26回学術集会
    • Place of Presentation
      東京
    • Year and Date
      2011-06-25
  • [Presentation] 光受容イオンチャネル、チャネルロドプシンの光受容-チャネル構造・機能連関2011

    • Author(s)
      八尾寛
    • Organizer
      CBI学会第317回研究講演会
    • Place of Presentation
      東京
    • Year and Date
      2011-05-27
  • [Presentation] Localization of the actin binding protein, coactosin that expressed in the developing chick ciliary ganglion2011

    • Author(s)
      細島頌子、侯旭濱、仲村春和、石塚徹、八 尾寛
    • Organizer
      第44回 日本発生生物学会年会
    • Place of Presentation
      沖縄
    • Year and Date
      2011-05-19
  • [Book] Muscle Optogenetics: Controlling muscle functions with light. In: Muscle Cells (Ed, Benigno Pezzo)2013

    • Author(s)
      Asano T, Ishizuka T, Yawo H
    • Total Pages
      127-135
    • Publisher
      Nova Science Publisher Inc.Hauppauge NY
  • [Book] Whole-Cell Patch Method" Patch-Clamp Techniques: From Beginning to Advanced Protocols (Springer Protocols Handbooks) (Ed. Yasunobu Okada)2012

    • Author(s)
      Hiromu Yawo
    • Total Pages
      43-69
    • Publisher
      Springer Verlag, Japan
  • [Remarks] 1.日本経済新聞(朝刊,科学欄),光が照らす脳の迷宮.2012年7月1日. 新聞報道

  • [Remarks] 2.日経産業新聞、皮膚で光感じるネズミ‐触角の仕組み解明に活用、2012年3月21日 新聞報道

  • [Remarks] 3.朝日新聞(朝刊、科学欄)、手足の皮膚で光を感知、2012年3月15日 新聞報道

  • [Remarks] 4.河北新報(朝刊)、皮膚で光を感知、東北大グループがマウス使い発見応用期待、2012年03月09日 新聞報道

  • [Patent(Industrial Property Rights)] ラット脳内光誘発けいれんモデル2012

    • Inventor(s)
      大沢 伸一郎,岩崎 真樹,虫明 元, 八尾 寛 ,冨永 悌二,古澤 義人
    • Industrial Property Rights Holder
      東北大学
    • Industrial Property Number
      PCT 出願PCT/JP2012/580
    • Filing Date
      2012-09-12
  • [Patent(Industrial Property Rights)] ラット脳内光誘発けいれんモデル2011

    • Inventor(s)
      大沢 伸一郎,岩崎 真樹,虫明 元, 八尾 寛 ,冨永 悌二,古澤 義人
    • Industrial Property Rights Holder
      東北大学
    • Industrial Property Number
      特許、特願2011-198126
    • Filing Date
      2011-09-12

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Published: 2014-09-25   Modified: 2015-11-04  

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