Elucidation of hypotonicity-induced suppression mechanism of vasopressin secretion through identification of hypoosmolarity sensor

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659118
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: National Institute for Physiological Sciences
• Principal Investigator: OKADA Yasunobu 生理学研究所, 所長 (10025661)
• Co-Investigator(Kenkyū-buntansha): SATO Kaori (NUMATA Kaori) 生理学研究所, 細胞器官研究系, NIPS リサーチフェロー (60614196)
• Co-Investigator(Renkei-kenkyūsha): UETA Yoichi 産業医科大学, 医学部, 教授 (10232745)
• Project Period (FY): 2011 – 2012
• Keywords: 浸透圧センサー / バソプレシン視索上核 / アニオンチャネル / タウリン / グリシンレセプター

Research Abstract

Arginine-vasopressin (AVP), an antidiuretic hormone, is secreted from AVP neurons in a manner sensitive to body fluid osmolarity. The current view for the hypotonicity-induced suppression mechanism consists of following two hypotheses: Reduced depolarization due to inactivation of stretch-inactivated cation channel (SIC) in osmotically swollen AVP neurons, and increased hyperpolarization due to glycine receptor (GlyR) activation in AVP neurons in response to taurine released from osmotically swollen glial cells. In rat AVP neurons, in the present study, the SIC activity was not observed, and taurine-induced activation of GlyR brought about depolarization but not hyperpolarization under hypotonic conditions. Also, the present study showed that taurine-releasing anion channel activated by cell swelling serves as a sensor for hypoosmolarity.

Research Products

• [Journal Article] Volume-sensitive anion channels mediate osmosensitive glutathione release from rat thymocytes (2012)
  • Author(s): R.Z. Sabirov, R.S. Kurbannazarova, N.R. Melanova, Y. Okada
  • Journal Title: PLoS One Volume: 8(1) Pages: 227,3498-3510
  • DOI: doi:10.1002/jcp.24050
  • Peer Reviewed
• [Journal Article] Involvements of the ABC protein ABCF2 and α-actinin-4 in regulation of cell volume and anion channels in human epithelial cells (2012)
  • Author(s): K. Sato, T. Numata, T. Saito, Y. Ueta & Y. Okada
  • Journal Title: Journal of Cellular Physiology Pages: 227,3498-3510
  • DOI: doi:10.1002/jcp.24050
  • Peer Reviewed
• [Journal Article] V2 receptor-mediated autocrine role of somatodendritic releas e of AVP in rat vasopressin neurons under hypo-osmotic conditions (2012)
  • Author(s): K. Sato, T. Numata, T. Saito, Y. Ueta & Y. Okada
  • Journal Title: Journal of Cellular Physiology Volume: 227 Pages: 3498-3510
  • DOI: doi:10.1002/jcp.24050
  • Peer Reviewed
• [Presentation] sensing mechanism in arginine-vasopressin neurons : Reexamination on the taurine hypothesis (2013)
  • Author(s): K. Sato-Numata, Y. Ueta, Y. Okada
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: 東京
  • Year and Date: 2013-03-28
• [Presentation] Multiple activation and regulation mechanisms of the volume-sensitive outwardly rectifying anion channel (2012)
  • Author(s): Y. Okada
  • Organizer: Multiple activation and regulation mechanisms of the volume-sensitive2012 International Ion Channel Conference "The New World of Anion and Cation Channels"outwardly rectifying anion channel
  • Place of Presentation: Jeju Island (Korea)
  • Year and Date: 2012-08-24
• [Presentation] Acid-sensing ion channels in body fluid homeostasis (2012)
  • Author(s): T. Ohbuchi, K. Sato, Y. Okada, H. Suzuki, Y. Ueta
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 松本
  • Year and Date: 2012-03-30
• [Presentation] Volume-regulatory autocrine action of vasopressin released from somato and dendrites in vasopressin neurons is mediated by the V2 receptor and cAMP (2011)
  • Author(s): K. Sato, T. Numata, T. Saito, Y. Ueta, Y. Okada
  • Organizer: Cell Volume regulation Meeting
  • Place of Presentation: Tubingen, Germany
  • Year and Date: 2011-09-06
• [Book] Patch Clamp Techniques - From Beginning to Advanced Protocols (2011)
  • Author(s): 岡田泰伸
  • Total Pages: 439
  • Publisher: Springer
• [Book] 最新パッチクランプ実験技術法 (2011)
  • Author(s): 岡田泰伸
  • Total Pages: 274
  • Publisher: 吉岡書店
• [Remarks] 生理学研究所HP&機能協関研究部門 HP:公開日 2013.01.31 抗酸化物質グルタチオンが細胞から放出 される「通り道」を発見:Sabirov RZ, Kurbannazarova RS, Melanova NR, Okada Y. Volume-sensitive anion channels mediate osmosensitive glutathione release from rat thymocytes. PLoS One, 2013
• [Remarks] 生理学研究所HP&機能協関研究部門 HP:公開日 2012.01.24 細胞容積調節におけるABCF2 とα-アク チニン-4 の分子間相互作用の役割: Ando-Akatsuka Y, Shimizu T, Numata T, Okada Y. Involvements of the ABC protein ABCF2 and α-actinin-4 in regulation of cell volume and anion channels in human epithelial cells. J Cell Physiol, 2012.