Challenge to reveal a novel role of central vasopressin and oxytocin neurons by optogenetic approach

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659127
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: UETA Yoichi 産業医科大学, 医学部, 教授 (10232745)
• Project Period (FY): 2011 – 2012
• Keywords: 光遺伝学 / バゾプレッシン / オキシトシン / トランスジェニック動物 / ニューロン

Research Abstract

Neurohypophysial hormones are synthesized in the magnocellular neurosecretory cells (MNCs) localized in the hypothalamus, and they are secreted not only in the systemic circulation but also in the central nervous system. This study tried the optogenetic approaches to express the light-sensitive proteins in the MNCs to control neuronal activity by light exposure. The light-sensitive proteins were expressed in the transgenic rats. The functional evaluation should be performed by further study.

Research Products

• [Journal Article] A c-fos-monomeric red fluorescent protein 1 fusion transgene is differentially expressed in rat forebrain and brainstem after chronic dehydration and rehydration (2013)
  • Author(s): Yoshimura, M. Ohkubo, J. Katoh, A. Ohno, M. Ishikura, T. Kakuma, T. Yoshimatsu, H. Murphy, D. & Ueta, Y
  • Journal Title: Journal of Neuroendcrinology Volume: 25(5) Pages: 478-487
  • DOI: DOI:10.1111/jne.12022
  • Peer Reviewed
• [Journal Article] Visualization of the response in the central nervous system after nociceptive stimulation using transgenic animals (2012)
  • Author(s): Ishikura, T. Suzuki, H. Matsuura, T. Ohnishi, H. Nakamura, T. & Ueta, Y
  • Journal Title: Journal of UOEH Volume: 34(4) Pages: 315-321
  • DOI: doi:なし
  • Peer Reviewed
• [Journal Article] Pathophysiological function of oxytocin secreted by neuropeptides (2012)
  • Author(s): Hashimoto, H. Uezono, Y. & Ueta, Y
  • Journal Title: A mini review. Pathophysiology Volume: 19 Pages: 283-298
  • DOI: DOI:10.1016/j.pathophys.2012.07.005
  • Peer Reviewed
• [Journal Article] Expression of the c-fos-monomeric red fluorescent protein 1 fusion gene in the spinal cord and the hypothalamic paraventricular nucleus in transgenic rats after nociceptive stimulation (2012)
  • Author(s): Ishikura, T. Suzuki, H. Yoshimura, M. Ohkubo, J. Katoh, A. Ohbuchi, T. Ohno, M. Fujihara, H. Kawasaki, M. Ohnishi, H. Nakamura, T. & Ueta, Y
  • Journal Title: Brain Research Volume: 1479 Pages: 52-61
  • DOI: DOI:10.1016/j.brainres.2012.08.033
  • Peer Reviewed
• [Journal Article] The Hypothalamic-Neurohypophyseal System: From Genome to Physiology (2012)
  • Author(s): Murphy, D. Konopacka, A. Hindmarch, C. Paton, J. F. Sweedler, J. V. Gillette, M. U. Ueta, Y. Grinevich, V. Lozic M. & Japundzic-Zigon, N
  • Journal Title: Journal of Neuroendocrinology Volume: 24(4) Pages: 539-553
  • DOI: DOI:10.1111/j.1365-2826.2011.02241.x
  • Peer Reviewed
• [Journal Article] Induction of arginine vasopressin-enhanced green fluorescent protein expression in the locus coeruleus following kainic acid-induced seizures in rats (2012)
  • Author(s): Ohno, M. Fujihara, H. Iwanaga, M. Todoroki, M. Katoh, A. Ohbuchi, T. Ishikura, T. Hamamura, A. Hachisuka, K. & Ueta, Y
  • Journal Title: Stress Volume: 15(4) Pages: 435-442
  • DOI: DOI:10.3109/10253890.2011.637185
  • Peer Reviewed
• [Journal Article] Upregulation of arginine vasopressin synthesis in the rat hypothalamus after kainic acid-induced seizures (2011)
  • Author(s): Iwanaga, M. Ohno, M. Katoh, A. Ohbuchi, T. Ishikura, T. Fujihara, H. Nomura, M. Hachisuka, K. & Ueta, Y
  • Journal Title: Brain Research Volume: 1424 Pages: 1-8
  • DOI: DOI:10.1016/j.brainres.2011.09.030
  • Peer Reviewed
• [Journal Article] Highly visible expression of an oxytocin-monomeric red fluorescent protein 1 fusion gene in the hypothalamus and posterior pituitary of transgenic rats (2011)
  • Author(s): Katoh, A. Fujihara, H. Ohbuchi, T. Onaka, T. Hashimoto, T. Kawata, M. Suzuki, H. & Ueta, Y
  • Journal Title: Endocrinology, (Cover article of the issue) Volume: 152(7) Pages: 2768-2774
  • DOI: DOI:10.1210/en.2011-0006
  • Peer Reviewed
• [Journal Article] Possible contribution of pannexin channel to ATP-induced currents in vitro in vasopressin neurons isolated from the rat supraoptic nucleus (2011)
  • Author(s): Ohbuchi, T. Yokoyama, T. Saito, T. Ohkubo, J. Suzuki, H. Ishikura, T. Katoh, A. Fujihara, H. Hashimoto, H. Suzuki, H. & Ueta, Y
  • Journal Title: Brain Research Volume: 1394 Pages: 71-78
  • DOI: DOI:10.1016/j.brainres.2011.04.017
  • Peer Reviewed
• [Journal Article] Hypothalamic vasopressin response to stress and various physiological stimuli: Visualization in transgenic animal models (2011)
  • Author(s): Ueta, Y. Dayanithi, G. Fujihara, H
  • Journal Title: Hormones and Behavior Volume: 59(2) Pages: 221-226
  • DOI: DOI:10.1016/j.yhbeh.2010.12.007
  • Peer Reviewed
• [Presentation] ラット視索上核バゾプレッシンおよびオキシトシンの蛍光タンパク遺伝子導入による同定とカイニン酸応答の検討 (2013)
  • Author(s): 大久保 淳一、大淵 豊明、吉村 充弘、丸山 崇、石倉 透、松浦 孝紀、加藤 明子、鈴木 秀明、上田 陽一
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: タワーホール船堀(東京都)
  • Year and Date: 20130327-29
• [Presentation] 蛍光イメージングによるHPA軸の可視化への挑戦 (2013)
  • Author(s): 上田 陽一
  • Organizer: 第24回間脳・下垂体・副腎系研究会
  • Place of Presentation: ロシュ・ダイアグノスティックス株式会社本社(東京都)
  • Year and Date: 2013-03-23
• [Presentation] カイニン酸誘発けいれんモデルラットにおける視床下部および青斑核におけるバゾプレッシン遺伝子発現 (2012)
  • Author(s): 上田 陽一、大野 素子、岩永 勝、吉村 充弘、丸山 崇、石倉 透
  • Organizer: 第65回日本自律神経学会総会
  • Place of Presentation: 都市センタービル(東京都)
  • Year and Date: 20121025-26
• [Presentation] Florescent visualization of oxytocin neurons activated by systemic administration of CCK-8 in transgenic rats expressing a c-fos-eGFP and an oxytocin-mRFP1 fusion gene (2012)
  • Author(s): Ueta , Y. Katoh, A
  • Organizer: Neuroscience 2012
  • Place of Presentation: New Orleans Morial Convention Center,(New Orleans, USA)
  • Year and Date: 20121013-17
• [Presentation] 蛍光タンパクを用いたラット下垂体後葉ホルモンおよびニューロン活動の可視化 (2012)
  • Author(s): 上田 陽一
  • Organizer: 第83回日本動物学会大会
  • Place of Presentation: 大阪大学豊中キャンパス(大阪府)
  • Year and Date: 20120913-15
• [Presentation] Fluorescent visualization of vasopressin and oxytocin neurons in the hypothalamus and their terminals in the hypothalamus in transgenic rats (2012)
  • Author(s): Ueta, Y
  • Organizer: 14th International Congress of Histochemistry and Cytochemistry
  • Place of Presentation: Kyoto International Conference Center (Kyoto, Japan)
  • Year and Date: 20120826-29
• [Presentation] ラットオキシトシン産生ニューロンの赤色蛍光タンパクによる可視化および生理学的刺激に対する反応性の検討 (2012)
  • Author(s): 上田 陽一、加藤 明子、吉村 充弘、石倉 透、鈴木 秀明
  • Organizer: 第85回日本内分泌学会学術総会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 20120419-21
• [Presentation] 体液調節中枢における酸感受性イオンチャネル (2012)
  • Author(s): 大淵 豊明、佐藤 かお里、岡田 泰伸、鈴木 秀明、上田 陽一
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 長野県松本文化会館(長野県)
  • Year and Date: 20120329-31
• [Presentation] ストレスと神経内分泌 (2012)
  • Author(s): 上田 陽一
  • Organizer: 第51回日本心身医学会九州地方会
  • Place of Presentation: 産業医科大学(福岡県)
  • Year and Date: 20120217-18
• [Presentation] Effects of kainic acid on arginine vasopressin (AVP) synthesis in vivo and an isolated AVP neuron in vitro (2012)
  • Author(s): Ueta, Y. Iwanaga, M. Ohkubo, J
  • Organizer: Neurophysiology of Neurosecretory Neurons Workshop
  • Place of Presentation: Tulane University (New Orleans, USA)
  • Year and Date: 2012-10-12
• [Presentation] バゾプレッシン産生ニューロンにおけるカイニン酸受容体サブタイプについての電気生理学的検討 (2011)
  • Author(s): 大久保 淳一、大淵 豊明、鈴木 秀明、上田 陽一
  • Organizer: 第38回日本神経内分泌学会
  • Place of Presentation: 都道府県会館(東京都)
  • Year and Date: 20111125-26
• [Presentation] オキシトシン-mRFPトランスジェニックラットにおけるナトリウムおよび絶食負荷の検討 (2011)
  • Author(s): 加藤 明子、吉村 充弘、石倉 透、鈴木 秀明、上田 陽一
  • Organizer: 第32回日本肥満学会
  • Place of Presentation: 淡路夢舞台国際会議場(兵庫県)
  • Year and Date: 20110923-24
• [Presentation] Highly visible expression of an oxytocin- monomeric red fluorescent protein 1 fusion gene in the hypothalamus and posterior pituitary of transgenic rats (2011)
  • Author(s): Katoh, A. Fujihara, H. Ohbuchi, T. Onaka, T. Hashimoto, T. Kawata, M. Suzuki, H. & Ueta, Y
  • Organizer: 9th World Congress on Neurohypophysial Hormones
  • Place of Presentation: Northeastern University (Boston, USA)
  • Year and Date: 20110727-30
• [Presentation] Fluorescent visualization of vasopressin- and oxytocin neurons under physiological and pathophysiological conditions (2011)
  • Author(s): Ueta, Y
  • Organizer: 9th World Congress on Neurohypophysial Hormones. Symposium
  • Place of Presentation: Northeastern University (Boston, USA)
  • Year and Date: 20110727-30
• [Presentation] トランスジェニックラットを用いた下垂体後葉ホルモンの可視化 (2011)
  • Author(s): 上田 陽一
  • Organizer: 日本顕微鏡学会第67回学術講演会シンポジウム
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 20110516-18
• [Presentation] オキシトシン-mRFPトランスジェニックラットへの浸透圧負荷の検討 (2011)
  • Author(s): 加藤 明子、藤原 広明、石倉 透、大淵 豊明、鈴木 秀明、上田 陽一
  • Organizer: 第84回日本内分泌学会学術総会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 20110421-23
• [Presentation] カイニン酸誘発モデルラットにおける視床下部バゾプレッシン遺伝子発現の亢進 (2011)
  • Author(s): 岩永 勝、大野 素子、大久保 淳一、加藤 明子、吉村 充弘、石倉 透、上田 陽一
  • Organizer: 第11回日本内分泌学会九州地方会
  • Place of Presentation: 福岡大学病院(福岡県)
  • Year and Date: 2011-08-27