2013 Fiscal Year Final Research Report

Development of novel chemical mutagenesis assay using budding yeast

Research Project

Project/Area Number	23659132
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	General pharmacology
Research Institution	Nagasaki University (2013) University of Tsukuba (2011-2012)
Principal Investigator	MASUMOTO HIROSHI 長崎大学, 医歯(薬)学総合研究科, 講師 (80423151)
Project Period (FY)	2011 – 2012
Keywords	創薬; ゲノム薬理学
Research Abstract	Exclusion of chemicals to cause DNA mutagenesis is important to prevent the incidence of cellular tumoregenesis. The AMS test using genetically-modificated bacteria has been widely employed to monitor DNA mutagenesis of chemicals. However, this assay may not work to detect the chemicals to cause DNA mutagens for eukaryote only. We developed the novel AMS test using the budding yeast as a eukaryote (the AMS yeast). The AMS yeast has the deletion of genes encoding protein pump to exclude chemicals from cells and can monitor the DNA mutagenesis caused by chemicals. Using this AMS budding yeast strain, we can detect DNA mutagens has been detected by traditional AMS test only, but also chemiclas to cause DNA mutagen restricted to eukaryote.