2012 Fiscal Year Final Research Report
Application of a cell-free protein display technology to the high-throughput functional proteomic screen for regulators of neuron-glia interaction
| Project/Area Number |
23659141
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General pharmacology
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NEMOTO Naoto 埼玉大学, 理工学研究科, 准教授 (60509727)
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| Co-Investigator(Renkei-kenkyūsha) |
KASHIYAMA Taku 順天堂大学, 医学部, 助教 (90338343)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 神経科学 / プロテオーム / 組換え抗体 / 薬理学 |
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| Research Abstract |
Fluorophore-assisted light inactivation (FALI) in combination with a cell-based assay is a uniquemethod for functional proteomic screen to search for regulators of a specific cellular function. One of the major problems with the method is the construction of antibody libraries that contain high-affinity binders to cell surface membrane proteins as potential targets. To overcome barriers to efficiency, we used a cell-free antibody display method. We tried to optimize the in vitro expression of the recombinant antibody that is linked to its genotype and the neuregulin-erbB model system for pilot-scale screening ofpotential regulators for neuron-glia interaction
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