2012 Fiscal Year Final Research Report
Establish of iPS by expression of microRNA
| Project/Area Number |
23659148
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | miRNA / iPS / 分化 |
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| Research Abstract |
miRNA expression pattern of human and mouse iPS was examined by qPCR array followed by bioinformatics, and a list of miRNA, which are expressing highly in iPS, was constructed. Expression vector of these miRNA was made, and they were introduced into MEF, immature iPS, and embryoid body to examine effects of these miRNA for efficiency to establish iPS, immaturity of iPS, and differentiation ability. Several types of expression vector had been constructed, and finally we got a vector expressing miRNA or its antagonized sequence stablyand with high level.
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Research Products
(9 results)
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[Journal Article] In vitro cell subtype-specific transduction of adeno-associated virus in the mouse and marmoset retinal explant culture2012
Author(s)
Baba, Y., Satoh, S., Otsu, M., Sasaki, E., Okada, T., and Watanabe, S.
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Journal Title
Biochimie
Volume: 94
Pages: 2716-2722
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[Journal Article] Sox9is upstream of microRNA-140 in cartilage2011
Author(s)
Nakamura, Y., He, X., Kato, H., Wakitani, S., Kobayashi, T., Watanabe, S., Iida, A., Tahara, H., Warman, M. L., Watanapokasin, R., Postlethwait, J.H.
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Journal Title
Appl. Biochem. Biotechnol
Volume: 166
Pages: 64-71
Peer Reviewed
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