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2012 Fiscal Year Final Research Report

Comprehensive Analysis of Ubiquitome during DNA damage response

Research Project

  • PDF
Project/Area Number 23659152
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General medical chemistry
Research InstitutionKyoto University

Principal Investigator

TAKEDA Shunichi  京都大学, 大学院・医学研究科, 教授 (60188191)

Co-Investigator(Kenkyū-buntansha) SASANUMA Hiroyuki  京都大学, 大学院・医学研究科, 准教授 (00531691)
Project Period (FY) 2011 – 2012
Keywordsプロテオミクス / ユビキチン / ユビキトーム / DNA修復 / 質量分析 / SILAC
Research Abstract

The objective of this study is to establish the protocol thatensures the sensitive analysis of ubiquitome for the chicken DT40 cell line. We havemet the objective, and identified a number of ubiquitylated proteins.

  • Research Products

    (5 results)

All 2013 2012 2011

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (4 results)

  • [Journal Article] Interference in DNAreplication can cause mitoticchromosomal breakage unassociatedwith double-strand breaks2013

    • Author(s)
      Fujita M, Sasanuma H, Yamamoto KN,Harada H, Kurosawa A, Adachi N, OmuraM, Hiraoka M, Takeda S, Hirota K.
    • Journal Title

      PLoS One

      Volume: 8 Pages: e60043

    • DOI

      DOI:10.1371/journal.pone.0060043

    • Peer Reviewed
  • [Presentation] 遺伝学的手法を応用した、新規抗がん剤のスクリーニングとその作用機序の解析2013

    • Author(s)
      武田俊一
    • Organizer
      金沢大学薬学シンポジウム2012-DNA損傷応答を標的とした革新的がん創薬研究-
    • Place of Presentation
      金沢
    • Year and Date
      2013-02-19
  • [Presentation] Genetic and Biocheicalevidence for translesion DNAsynthesis by replicative DNApolymerase delta2012

    • Author(s)
      Takeda S
    • Organizer
      Gordon ResearchConference, Mutagenesis
    • Place of Presentation
      Newport, USA
    • Year and Date
      20120819-24
  • [Presentation] Xpf and Mus81 are requiredto process homologous recombinationintermediates2012

    • Author(s)
      Takeda S
    • Organizer
      EMBO Workshop,Structure-specific nucleases in DNAreplication and repair
    • Place of Presentation
      Hyeres, France.
    • Year and Date
      2012-09-19
  • [Presentation] Complex functionalrelationship of the UBC13 E2ubiquitin conjugating enzyme, and theRAD18 and RNF8 E3 ubiquitin ligases,FASEB Summer Research Conferences2011

    • Author(s)
      Takeda S
    • Organizer
      Genetic Recombination & GenomeRearrangements
    • Place of Presentation
      Steamboat Springs, USA
    • Year and Date
      20110724-29

URL: 

Published: 2014-08-29   Modified: 2014-10-07  

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