2013 Fiscal Year Final Research Report
In vivo analysis of cell polarity during cell-cell fusion
Project/Area Number |
23659162
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
General medical chemistry
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Research Institution | Hokkaido University (2013) Keio University (2011-2012) |
Principal Investigator |
OIKAWA Tsukasa 北海道大学, 医学(系)研究科(研究院), 特任講師 (20457055)
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Project Period (FY) |
2011 – 2013
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Keywords | 細胞極性 |
Research Abstract |
Among various phosphatidylinositols (PI) on the plasma membrane, the most abundant product, PI(4,5)P2 and locally-produced PI(3,4,5)P3 and PI(3,4)P2 seem to be particularly important in polarity formation of the cells. The PH domain of PLC delta1 binds to PI(4,5)P2 while the PH domain of Akt binds to PI(3,4)P2 and PI(3,4,5)P3, which is locally produced depending on PI3-kinase activity. By using these domains, I succeeded in observing the polarity of fusing cells. To apply this system in vivo, KH2 ES cells which can express those domains fused to GFP or RFP in response to doxycyclin were generated. Now that genetic quality of the ES cells were confirmed, the mice who express the polarity probe is being generated.
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[Presentation] アダプター分子Tks5 依存的なポドソーム/インベードポディア形成と, これを介した破骨/がん細胞融合の解析2013
Author(s)
及川 司, 尾山大明, 秦 裕子, 中村敦子, 大西伸幸, 上原俊介, 宇田川信之, 山田健人, 佐谷秀行, 松尾光一
Organizer
第86回日本生化学会大会
Place of Presentation
パシフィコ横浜(横浜市)
Year and Date
20130911-13
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