In vivo analysis of cell polarity during cell-cell fusion

2013 Fiscal Year Final Research Report

• Project/Area Number: 23659162
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Hokkaido University (2013); Keio University (2011-2012)
• Principal Investigator: OIKAWA Tsukasa 北海道大学, 医学(系)研究科(研究院), 特任講師 (20457055)
• Project Period (FY): 2011 – 2013
• Keywords: 細胞極性

Research Abstract

Among various phosphatidylinositols (PI) on the plasma membrane, the most abundant product, PI(4,5)P2 and locally-produced PI(3,4,5)P3 and PI(3,4)P2 seem to be particularly important in polarity formation of the cells. The PH domain of PLC delta1 binds to PI(4,5)P2 while the PH domain of Akt binds to PI(3,4)P2 and PI(3,4,5)P3, which is locally produced depending on PI3-kinase activity. By using these domains, I succeeded in observing the polarity of fusing cells. To apply this system in vivo, KH2 ES cells which can express those domains fused to GFP or RFP in response to doxycyclin were generated. Now that genetic quality of the ES cells were confirmed, the mice who express the polarity probe is being generated.

Research Products

• [Journal Article] Acquired expression of NFATc1 downregulates E-cadherin and promotes cancer cell invasion (2013)
  • Author(s): Oikawa T, Nakamura A, Onishi N, Yamada T, Matsuo K, Saya H
  • Journal Title: Cancer Res Volume: 73(16) Pages: 5100-5109
  • DOI: 10.1158/0008-5472.CAN-13-0274
  • Peer Reviewed
• [Journal Article] IRSp53 mediates podosome formation via VASP in NIH-Src cells (2013)
  • Author(s): Oikawa T, Okamura H, Dietrich F, Senju Y, Takenawa T, Suetsugu S
  • Journal Title: PLoS ONE Volume: 8(3) Pages: e60528
  • DOI: 10.1371/journal.pone.0060528
  • Peer Reviewed
• [Journal Article] Regulation of osteoclasts by membrane-derived lipid mediators (2013)
  • Author(s): Oikawa T, Kuroda Y, Matsuo K
  • Journal Title: Cell Mol Life Sci Volume: 70(18) Pages: 3341-3353
  • DOI: 10.1007/s00018-012-1238-4
  • Peer Reviewed
• [Journal Article] Possible role of IRTKS in Tks5-driven osteoclast fusion (2012)
  • Author(s): Oikawa T and Matsuo K
  • Journal Title: Commun Integr Biol Volume: 5(5) Pages: 511-515
  • DOI: 10.4161/cib.21252
  • Peer Reviewed
• [Journal Article] Tks5-dependent formation of circumferential podosomes/invadopodia mediates cell-cell fusion (2012)
  • Author(s): Oikawa T, Oyama M, Kozuka-Hata H, Uehara S, Udagawa N, Saya H, Matsuo K
  • Journal Title: J Cell Biol Volume: 197(4) Pages: 553-568
  • DOI: 10.1083/jcb.201111116
  • Peer Reviewed
• [Presentation] アダプター分子Tks5 依存的なポドソーム/インベードポディア形成と, これを介した破骨/がん細胞融合の解析 (2013)
  • Author(s): 及川 司, 尾山大明, 秦 裕子, 中村敦子, 大西伸幸, 上原俊介, 宇田川信之, 山田健人, 佐谷秀行, 松尾光一
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 20130911-13
• [Presentation] がん-マクロファージ細胞融合による, がん悪性化機構の解析 (2013)
  • Author(s): 及川 司
  • Organizer: 第2回蛍光バイオイメージングミニシンポジウム
  • Place of Presentation: 北海道大学電子科学研究所(札幌市)
  • Year and Date: 2013-09-20
  • Invited
• [Presentation] Tks5-dependent formation of circumferential podosomes/invadopodia mediates cell-cell fusion (2012)
  • Author(s): Tsukasa Oikawa, Masaaki Oyama, Hiroko Kozuka-Hata, Shunsuke Uehara, Nobuyuki Udagawa, Hideyuki Saya, Koichi Matsuo
  • Organizer: ASBMR (American Society for Bone Mineral Research) 2012 Annual Meeting
  • Place of Presentation: Minneapolis Convention Center(Minneapolis, USA)
  • Year and Date: 20121011-15
• [Presentation] Tks5-dependent formation of circumferential podosomes/invadopodia mediates cell-cell fusion (2012)
  • Author(s): Tsukasa Oikawa, Shunsuke Uehara, Nobuyuki Udagawa, Koichi Matsuo
  • Organizer: 第30回日本骨代謝学会
  • Place of Presentation: 京王プラザホテル(東京都新宿区)
  • Year and Date: 20120719-21
• [Presentation] NFATc1 promotes cancer cell invasion via altered E-cadherin regulation (2012)
  • Author(s): Tsukasa Oikawa, Atsuko Nakamura, Koichi Matsuo, Hideyuki Saya
  • Organizer: 第64回日本細胞生物学会
  • Place of Presentation: 神戸国際会議場(神戸市)
  • Year and Date: 20120528-31
• [Presentation] Tks5-dependent formation of circumferential podosomes mediates cell-cell fusion (2011)
  • Author(s): Tsukasa Oikawa, Masaaki Oyama, Hiroko Kozuka-Hata, Shunsuke Uehara, Nobuyuki Udagawa, Hideyuki Saya, Koichi Matsuo
  • Organizer: Bio-Rheumatology International Congress
  • Place of Presentation: Sheraton Grande Tokyo Bay Hotel (Urayasu, Japan)
  • Year and Date: 20111114-16
• [Presentation] Tks5-dependent formation of circumferential podosomes mediates cell-cell fusion (2011)
  • Author(s): Tsukasa Oikawa, Masaaki Oyama, Hiroko Kozuka-Hata, Shunsuke Uehara, Nobuyuki Udagawa, Hideyuki Saya, Koichi Matsuo
  • Organizer: Podosomes, Invadopodia and Focal Adhesions in Physiology and Pathology
  • Place of Presentation: Ayre Gran Hotel Colon(Madrid, Spain)
  • Year and Date: 20110918-21
• [Presentation] Tks5-dependent formation of circumferential podosomes mediates cell-cell fusion (2011)
  • Author(s): Tsukasa Oikawa, Masaaki Oyama, Hiroko Kozuka-Hata, Hideyuki Saya, Koichi Matsuo
  • Organizer: 第29回日本骨代謝学会
  • Place of Presentation: 大阪国際会議場(大阪市)
  • Year and Date: 20110728-30
• [Presentation] Tks5-dependent formation of circumferential podosomes mediates cell-cell fusion (2011)
  • Author(s): Tsukasa Oikawa, Masaaki Oyama, Hiroko Kozuka-Hata, Hideyuki Saya, Koichi Matsuo
  • Organizer: 第63回日本細胞生物学会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 20110627-29
• [Remarks]
  • URL: http://www.careerpath-prj.keio.ac.jp/kanrinmaru/scholar/oikawa/