2012 Fiscal Year Final Research Report
Development of a novel therapy targeting tight-junction protein occludin against hepatitis C
| Project/Area Number |
23659187
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
CHIBA Hideki 福島県立医科大学, 医学部, 教授 (00295346)
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| Co-Investigator(Kenkyū-buntansha) |
TOMIKAWA Naoki 福島県立医科大学, 医学部, 講師 (80468587)
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| Project Period (FY) |
2011 – 2012
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| Keywords | タイト結合 / C 型肝炎ウィルス / 感染症 / 肝癌 / トうンスレーショナルリサーチ / 抗体医薬 |
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| Research Abstract |
To prevent and treat Hepatitis C virus (HCV) infection, we generated a novel monoclonal antibody against human occludin. 15 clones of the purified antibodies showed no toxicity in the human hepatocellular carcinoma cell line Huh7.5.1. These antibodies did not inhibit HCV infection in Huh7.5.1 monolayer culture. However, when the fluorescent labeling antibody was applied to the lower chamber, the signal was detected along cell borders of Huh7.5.1 cells cultured in the upper chamber, suggesting that the novel antibody binds to occludin in living cells.
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[Journal Article] Laminin a1 is essential for mouse cerebellar development.2012
Author(s)
Ichikawa-Tomikawa N, Ogawa J, Douet V, Xu Z, Kamikubo Y, Sakurai T, Kohsaka S, Chiba H, Hattori N, Yamada Y,Arikawa-Hirasawa E
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Journal Title
Matrix Biol
Volume: 31
Pages: 17-28
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[Journal Article] The transient potential channel TRPV6 is dynamically expressed in bone cells but in not crucial for bone mineralization in mice2012
Author(s)
van der Eerden BC, Weissgerber P, Fratzl-Zelman N, Olausson J, Hoenderop JG,Schreuders-Koedam M, Eijken M, Roschger P, de Vries TJ, Chiba H, Klaushofer K, Flockerzi V, Bindels RJ, Freichel M, van Leeuwen JP
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Journal Title
J Cell Physiol
Volume: 227
Pages: 1951-1959
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