2013 Fiscal Year Final Research Report
Elucidation of malaria erythrocyte invasion system using stem cell techonology
| Project/Area Number |
23659208
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Obihiro University of Agriculture and Veterinary Medicine (2012-2013)
The University of Tokyo (2011) |
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Principal Investigator |
KATO Kentaro 帯広畜産大学, 原虫病研究センター, 特任准教授 (30401178)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJI Koichiro 東京大学, 医科学研究所, 准教授 (50179991)
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| Project Period (FY) |
2011 – 2012
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| Keywords | マラリア原虫 / 幹細胞 / 赤血球 |
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| Research Abstract |
We confirmed the invasion of Plasmodium falciparum using erythrocyte like cells from human umbilical cord blood hematopoietic stem cell and mouse ES cells. The establishment of effective production system of mature erythrocyte from stem cell has been mainly proceeded. Before the treatment of ES cell and iPS cell, the technique that CD34 positive hematopoietic stem cell are isolated from umbilical cord blood and cultured have been mastered. Afterwards, we investigated the effective condition to lead enucleation with arrangement of class, concentration of cytokine, and culture period.
Moreover, to produce the differentiated erythrocyte mutant with specific protein deficient, we tried to establish the trancfection system of differentiated erythrocyte using lentivirus vecter.
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[Journal Article] Gellan sulfate inhibits Plasmodium falciparum growth and invasion of red blood cells in vitro2014
Author(s)
Recuenco FC, Kobayashi K, Ishiwa A, Enomoto-Rogers Y, Fundador NGV, Sugi T, Takemae H, Iwanaga T, Murakoshi F, Gong H, Inomata A, Horimoto T, Iwata T, Kato K
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Journal Title
Scientific Reports. (Nature Publishing Group), (帯広畜産大学プレスリリース、東京大学大学院農学生命科学研究科研究成果(2014 年4 月23 日)、十勝毎日新聞記事掲載(2014年5 月2 日1 面)、UTokyo Research 記事掲載(2014 年5 月14 日)、北海道新聞記事掲載(2014 年5 月22 日朝刊29 面))
Volume: 4
Pages: 4723
Peer Reviewed
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[Journal Article] Analyses of interactions between heparin and the apical surface proteins of Plasmodium falciparum2013
Author(s)
Kobayashi K, Takano R, Takemae H, Sugi T, Gong H, Recuenco FC, Iwanaga T, Horimoto T, Akashi H, Kato K
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Journal Title
Sci Rep. (Nature Publishing Group), (帯広畜産大学プレスリリース、東京大学大学院農学生命科学研究科プレスリリース(2013 年11 月13 日)、Todai Research記事掲載(2013 年11 月21 日)、日経産業新聞記事掲載(2013 年11 月21 日11 面)、十勝毎日新聞記事掲載(2013 年11 月25日24 面)、北海道新聞記事掲載(2013 年11 月26 日朝刊25 面))
Volume: 3
Pages: 3178
Peer Reviewed
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[Journal Article] Characterization of Plasmodium falciparum cdc2-related kinase and the effects of a CDK inhibitor on the parasites in erythrocytic schizogony2013
Author(s)
Iwanaga T, Sugi T, Kobayashi K, Takemae H, Gong H, Recuenco FC, Ishiwa A, Horimoto T, Akashi H, Kato K
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Journal Title
Parasitol Int, (Malaria Nexus 記事掲載(2013 年6 月6日)
Volume: 62
Pages: 423-430
Peer Reviewed
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[Journal Article]2012
Author(s)
Kobayashi K, Takemae H, Sugi T, Gong H, Recuenco F, Iwanaga T, Horimoto T, Akashi H, Kato K
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Journal Title
Jpn J Vet Parasitol
Volume: 11
Pages: 23
Peer Reviewed
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