Development of a direct transfection method in Plasmodium falciparum

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659210
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Mie University
• Principal Investigator: YUDA Masao 三重大学, 大学院・医学系研究科, 教授 (90293779)
• Project Period (FY): 2011 – 2012
• Keywords: 原虫

Research Abstract

At present, genetic manipulation of Plasmodium falciparum, the most important human malaria parasite is carried out with indirect transfection method that is of low efficiencies. In this study we attempted to develop a direct transfection method in this parasite andimprove the efficiencies. By establishing a method to purify a large amount of mature schizonts and using a P. falciparum artificial chromosome, we succeeded in introducing a foreign DNA into the parasites with approximately 1000-fold efficiency compared to that with indirect transfection method. The method developed in this study will improve genetic manipulation of this parasite and have a great impact on this research field.

Research Products

• [Journal Article] Liver-specific protein 2: a Plasmodium protein exported to the hepatocyte cytoplasm and required for merozoite formation (2012)
  • Author(s): Orito, Y, T. Ishino, S. Iwanaga, T. Kato, I. Kaneko & M. Yuda
  • Journal Title: Molecular microbiology Volume: 87 Pages: 66-79
  • Peer Reviewed
• [Journal Article] a Transcription Factor That Is Critical for Malaria Liver Stage Development (2012)
  • Author(s): Iwanaga, S., T. Kato, I. Kaneko & M. Yuda
  • Journal Title: PloS one Volume: 7 Pages: e47557
  • Peer Reviewed
• [Journal Article] A high-coverage artificial chromosome library for the genome-wide screening of drug-resistance genes in malaria parasites (2012)
  • Author(s): Iwanaga, S., I. Kaneko & M. Yuda
  • Journal Title: Genome research Volume: 22 Pages: 985-992
  • Peer Reviewed
• [Journal Article] Centromere plasmid: a new genetic tool for the study of Plasmodium falciparum (2012)
  • Author(s): Iwanaga, S., T. Kato, I. Kaneko &Masao Yuda
  • Journal Title: PloS one Volume: 7 Pages: e33326
  • Peer Reviewed
• [Journal Article] Development of Memory CD8+ T Cells and Their Recall Responses during Blood-Stage Infection with Plasmodium berghei ANKA (2012)
  • Author(s): Miyakoda, M., D. Kimura, K. Honma, K. Kimura, M.Yud a & K. Yui
  • Journal Title: J Immunol Volume: 189 Pages: 4396-3404
  • Peer Reviewed
• [Journal Article] Reduction of malaria transmission by transgenic mosquitoes expressing an anti-sporozoite antibody in their salivary glands (2012)
  • Author(s): Megumi Sumitani, Katsumi Kasashima, Daisuke S. Yamamoto, Keita Yagi, M. Yuda,Hiroyuki Matsuoka and Shigeto Yoshida
  • Journal Title: Insect Mol Biol Volume: 22 Pages: 41-51
  • Peer Reviewed
• [Journal Article] Altered gene and protein expression in liver of the obese spontaneously hypertensive/NDmcr-cp rat (2012)
  • Author(s): Chang, J., S. Oikawa, G. Ichihara, Y. Nanpei, Y. Hotta, Y. Yamada, S. Tada-Oikawa, H. Iwahashi, E. Kitagawa, I. Takeuchi, M. Yuda & S. Ichihara
  • Journal Title: Nutrition & metabolism Volume: 9 Pages: 87
  • Peer Reviewed
• [Journal Article] Prevention of experimental cerebral malaria by Flt3 ligand during infection with Plasmodium berghei ANKA (2011)
  • Author(s): Tamura, T., K. Kimura, M. Yuda & K. Yui
  • Journal Title: Infection and immunity Volume: 79 Pages: 3947-3956
  • Peer Reviewed
• [Presentation] マラリア原虫転写因子AP2-Oのオーキネート形成に果たす役割 (2013)
  • Author(s): 金子伊澄、加藤知美、岩永史朗、油田正夫
  • Organizer: 第82回日本寄生虫学会大会
  • Place of Presentation: 東京医科歯科大学
  • Year and Date: 20130329-31
• [Presentation] マラリア原虫肝臓ステージの遺伝子発現を制御する転写因子の研究 (2012)
  • Author(s): 金子伊澄、岩永史朗、加藤知美、油田正夫
  • Organizer: 第81回日本寄生虫学会大会
  • Place of Presentation: 兵庫医科大学
  • Year and Date: 20120323-24
• [Remarks] 医動物学ホームページ
  • URL: http://www.medic.mie-u.ac.jp/idoubutsu/