2013 Fiscal Year Final Research Report
Development of antimalarial drug targeting plasmodium falciparum dynamin
| Project/Area Number |
23659213
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Okayama University |
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Principal Investigator |
TAKEI Kohji 岡山大学, 医歯(薬)学総合研究科, 教授 (40322226)
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Hiroshi 岡山大学, 大学院・医歯薬学総合研究科, 准教 (80325092)
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| Project Period (FY) |
2011 – 2013
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| Keywords | マラリア / アクチン / ダイナミン / コルタクチン / 阻害剤 |
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| Research Abstract |
By in vitro actin polymerization assay, we identified N'-(4-(diethylamino) benzylidene)-4-methoxybenzohydrazide (DBHA) as a dynamin inhibitor. Effects of DBHA on ruffle formation and cell migration were also examined, and the results on DBHA were published in a journal article.
Dynamin isoforms present in plasmodium falciparum, pfDyn1 and pfDyn2, were expressed in insect cells, and they were purified. Using these recombinant proteins, we demonstrated that both pfDyn1 and pfDyn2 have GTPase activity. It was also shown that pfDyn1 and pfDyn2 have an ability to deform lipid membranes. Furthermore, pfDyn2 inhibitor candidate molecules were determined by GTPase activity assay-based drug screening.
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Research Products
(28 results)
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[Journal Article] N'-[4-(dipropylamino)benzylidene]-2- hydroxybenzohydrazide is a dynamin GTPase inhibitor that suppresses cancer cell migration and invasion by inhibiting actin polymerization2013
Author(s)
Yamada, H., Abe, T., Li, S.A., Tago, S., Huang, P., Watanabe, M., Ikeda, S., Ogo, N., Asai, A., Takei, K
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Journal Title
Biochem Biophys Res Commun
Volume: 443
Pages: 511-517
DOI
Peer Reviewed
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[Journal Article] Stabilization of actin bundles by a dynamin 1/cortactin ring complex is necessary for growth cone filopodia2013
Author(s)
Yamada, H., Abe, T., Satoh, A., Okazaki, N., Tago, S., Kobayashi, K., Yoshida, Y., Oda, Y., Watanabe, M., Tomizawa, K., Matsui, H., and Takei, K
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Journal Title
J Neurosci
Volume: 33
Pages: 4514-4526
DOI
Peer Reviewed
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[Journal Article] REIC/Dkk-3-encoding adenoviral vector as a potentially effective therapeutic agent for bladder cancer2012
Author(s)
Hirata, T., Watanabe, M., Kaku, H., Kobayashi, Y., Yamada, H., Sakaguchi, M., Takei, K., Huh, N.H., Nasu, Y, Kumon, H
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Journal Title
Int J Oncol
Volume: 41
Pages: 559-564
DOI
Peer Reviewed
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[Journal Article] A novel gene expression system for detecting viable bladder cancer cells2012
Author(s)
Ueki, H., Watanabe, M., Kaku, H., Huang, P., Li, S.A., Ochiai, K., Hirata, T., Noguchi, H., Yamada, H., Takei, K., Nasu, Y, Kashiwakura Y, and Kumon H
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Journal Title
Int J Oncol
Volume: 41
Pages: 135-140
Peer Reviewed
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[Journal Article] The clathrin assembly protein PICALM is required for erythroid maturation and transferrin internalization in mice2012
Author(s)
Suzuki, M., Tanaka, H., Tanimura, A., Tanabe, K., Oe, N., Rai, S., Kon, S., Fukumoto, M., Takei, K., Abe, T., Matsumura, I., Kanakura, Y., and Watanabe, T
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Journal Title
PLoS One
Volume: 7
Pages: e31854
DOI
Peer Reviewed
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[Journal Article] Receptor sorting within endosomal trafficking pathway is facilitated by dynamic actin filaments2011
Author(s)
Ohashi, E., Tanabe, K., Henmi, Y., Mesaki, K., Kobayashi, Y., and Takei, K
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Journal Title
PLoS One
Volume: 6
Pages: e19942
DOI
Peer Reviewed
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[Patent(Industrial Property Rights)] フルボキサミンを配合することを特徴とする分子標的型抗癌剤(特に抗脳腫瘍治療薬剤:第二医薬品用途)2012
Inventor(s)
道上宏之, 林圭一郎, 松井秀樹, 山田浩司, 竹居孝二, 宮地弘幸, 浅井章良
Industrial Property Rights Holder
道上宏之, 林圭一郎, 松井秀樹, 山田浩司, 竹居孝二, 宮地弘幸, 浅井章良
Industrial Property Rights Type
特許
Industrial Property Number
特願2012-263317
Filing Date
2012-11-30
