2012 Fiscal Year Final Research Report
Development of a novel technique to monitor gene expression profiles of pathogenic bacteria during infection process
| Project/Area Number |
23659222
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | IVET / マイクロアレイ / 気管支敗血症菌 |
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| Research Abstract |
Analyses for gene expression of pathogens in response to the host environment provide clues to understanding the underlying events to establish the infectious diseases. In this study, we established a novel method named IVET-IP (in vivo expression tag immunoprecipitation system) to enable us to identify genes expressed in the host environment. By using this method, we determined genes expressed in Bordetella bronchiseptica in rats during infection process. As a result, we found that the in vivo expression pattern of bacterial virulence genes were quite different from that in vitro. In addition, at least 289 gene loci of Bordetella bronchiseptica were found to be expressed >2-fold in rats compared to in vitro.
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[Journal Article] Substrate specificity of Pasteurella multocidatoxin for α subunits of heterotrimeric G proteins2013
Author(s)
Orth JHC, Fester I, Siegert P, Weise M, Lanner U, Kamitani S, Tachibana T, Wilson BA, Schlosser A, Horiguchi Y, Aktories K
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Journal Title
FASEB J
Volume: 27
Pages: 832-842
DOI
Peer Reviewed
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[Journal Article] Crystal Structure of Clostridium perfringensEnterotoxin Displays Features of {beta}-Pore-forming Toxins2011
Author(s)
Kitadokoro K, Nishimura K, Kamitani S, Fukui-Miyazaki A, Toshima H, Abe H, Kamata Y, Sugita-Konishi Y, Yamamoto S, Karatani H, Horiguchi Y
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Journal Title
J Biol Chem
Volume: 286
Pages: 19549-19555
DOI
Peer Reviewed
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