Development of a novel virus targeting vector

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659236
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: TAKEDA Makoto 国立感染症研究所, ウイルス第三部, 部長 (40311401)
• Project Period (FY): 2011 – 2012
• Keywords: 細胞 / ウイルスベター

Research Abstract

The aim of this research is to generate a novel viral vector, which has the ability to accommodate multiple foreign genes and target specific cell types. The targeting will be achieved by mixing the vector with antibodies against specific surface antigens on cells to be targeted. (1) Using cells expressing Fc receptor, the antibody-dependent infection ability of measles virus vector was demonstrated. (2) A replication-incompetent virus vector accommodating multiple foreign genes was successfully generated. Using the vector, we have tried to produce iPS cells. However, so far, no iPS was produced, although expression of several reprograming (early) genes was observed.

Research Products

• [Journal Article] Functional and structural characterization of neutralizing epitopes of measles virus hemagglutinin protein. (2013)
  • Author(s): Tahara M, Ito Y, Brindley M, Ma X, He J, Xu S, Fukuhara H, Sakai K, Komase K, Rota P, Plemper R, Maenaka K, Takeda M
  • Journal Title: J. Virol Volume: 87 Pages: 666-75
• [Journal Article] The Receptor-binding Site of the Measles Virus Hemagglutinin Protein Itself Constitutes a Conserved Neutralizing Epitope. (2013)
  • Author(s): Tahara M, Ohno S, Sakai K, Ito Y, Fukuhara H, Komase K, Brindley MA, Rota PA, Plemper RK, Maenaka K, Takeda M
  • Journal Title: J Virol Volume: 87 Pages: 3583-6
• [Presentation] A structural and biochemical basis for the single serotype nature of measles virus (2012)
  • Author(s): Tahara M, Ito Y, Brindley MA, Ma XM, He JL, Xu ST, Fukuhara H, Sakai K, Ohno S, Komase K, Rota PA, Plemper RK, Maenaka K, Takeda M
  • Organizer: The 11th Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji Island, Hyogo, Japan
  • Year and Date: 20120911-14
• [Presentation] A novel non-transmissible measles virus vector with five iPS cell-inducing genes and a reporter gene (2012)
  • Author(s): Tahara M, Miura Y, Kurita R, Ryo A, Tani K, Takeda M
  • Organizer: 31st Annual Meeting for American Society for Virology
  • Place of Presentation: Madison, WI, USA
  • Year and Date: 20120721-25
• [Presentation] transmissible and cytosol-replicating RNA virus vector that encodes five iPS cell-inducing genes and a reporter gene (2012)
  • Author(s): Tahara M, Miura Y, Kurita R, Ryo A, Tani K, Takeda M
  • Organizer: International Society for Stem Cell Research (ISSCR) 11th Annual Meeting
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20120613-16
• [Presentation] Identification of conserved neutralizing epitopes of the measles virus hemagglutinin protein located in proximity and distal to the receptor-binding site (2011)
  • Author(s): Tahara M, Komase K, Ma XM, He JL, Yanagi Y, Maenaka K, Rota PA, Takeda M
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 20110911-16
• [Presentation] Conserved and variable antigenic sites on the mealses virus hemagglutinin protein (2011)
  • Author(s): Tahara M, Komase K, Yanagi Y, Maenaka K, Rota PA, Takeda M
  • Organizer: 30th Annual Meeting of American Scociety for Virology
  • Place of Presentation: Minneapolis, Minnesota
  • Year and Date: 20110716-20
• [Presentation] Measles virus escapes from neutralization at the cost of SLAM-binding activity (2011)
  • Author(s): Tahara M, Ma XM, He JL, Ito Y, Fukuhara H, Brindley MA, Sakai K, Yanagi Y, Komase K, Plemper RK, Rota PA, Maenaka K, Takeda M
  • Organizer: Atlanta Area 5th Paramyxer
  • Place of Presentation: University of Georgia, Atlanta, GA, USA
  • Year and Date: 2011-12-02
• [Presentation] Antigenic determinants of Measles Virus Hemagglutinin associated with single serotype (2011)
  • Author(s): Tahara M, Komase K, Ma XM, He JL, Yanagi Y, Maenaka K, Rota PA, and Takeda M
  • Organizer: 2011 Measles Virus Mini-Symposium
  • Place of Presentation: Mayo Clinic, Rochester, MN
  • Year and Date: 2011-07-15