Elucidation of behavior and functions of a dendritic cell subset with high crosspresenting activity

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659245
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Immunology
• Research Institution: Osaka University
• Principal Investigator: KAISHO Tsuneyasu 大阪大学, 免疫学フロンティア研究センター, 寄附研究部門教授 (60224325)
• Project Period (FY): 2011 – 2012
• Keywords: 樹状細胞 / クロスプレゼンテーション / ケモカイン受容体 / サブセット

Research Abstract

CD8α/CD103^<＋>CD11b^<- >dendritic cell (DC) is one DC subset, which is characterized by its high ability to ingest dead cells and crosspresent antigens to generate CD8 T cell responses. We have generated knock-in mice in which CD8α/CD103＋CD11bDC can be tracked and ablated and clarified in vivo distribution and functions of CD8α/CD103＋CD11bDC. The mice can be applied to various experimental models and should be useful to reveal the novel functions of CD8α/CD103＋CD11bDC.

Research Products

• [Journal Article] Double-stranded RNA of small intestinal commensal but not pathogenic bacteria triggers TLR3-mediated IFN-β production by dendritic cells (2013)
  • Author(s): T. Kawashima, A. Kosaka, H. Yan, G. Zijin, R. Uchiyama, R. Fukui, D. Kaneko; Y. Kumagai; D.-J. You, J. Carreras, S. Uematsu, M. H. Jang, O. Takeuchi, T. Kaisho, S. Akira, K. Miyake, H. Tsutsui, T. Saito, I. Nishimura, N. M. Tsuji
  • Journal Title: Immunity Volume: (in press)
  • Peer Reviewed
• [Journal Article] Critical Roles of a Dendritic Cell Subset Expressing a Chemokine Receptor, XCR1. (2013)
  • Author(s): C. Yamazaki, M. Sugiyama, T. Ohta, H. Hemmi, E. Hamada, I. Sasaki, Y. Fukuda, T. Yano, M. Nobuoka, T. Hirashima, A. Iizuka, K. Sato, T. Tanaka, K. Hoshino and T. Kaisho
  • Journal Title: J Immunol Volume: 190(12)
  • DOI: doi:10.4049/jimmunol.1202798
  • Peer Reviewed
• [Journal Article] Invariant NKT cells induce plasmacytoid DC cross-talk with conventional DCs for efficient memory CD8+ T cell induction. (2013)
  • Author(s): K. Shimizu, M. Asakura, J. Shinga, Y. Sato, S. Kitahara, K. Hoshino, T. Kaisho, S. P. Schonberger, T. Ezaki, S. -I. Fujii.
  • Journal Title: J. Immunol Volume: 190 Pages: 5609-5619
  • DOI: doi:10.4049/jimmunol.1300033
  • Peer Reviewed
• [Journal Article] Enhanced Apoptosis by Disruption of the STAT3-IκB-ζSignaling Pathway in Epithelial Cells Induces Sjogren's Syndrome-like Autoimmune Disease (2013)
  • Author(s): A. Okuma, K. Hoshino, T. Ohba, S. Fukushi, S. Aiba, S. Akira, M. Ono, T. Kaisho, T. Muta
  • Journal Title: Immunity Volume: 38 Pages: 450-460
  • DOI: doi.:10.1016/j.immuni.2012.11.016
  • Peer Reviewed
• [Journal Article] Panthogen sensors and chemokine receptors in dendric cell subsets. (2012)
  • Author(s): T. Kaisho
  • Journal Title: Vaccine Volume: 30 Pages: 7652-7657
  • URL: http://dx.doi.org/10.1016/j.vaccine.2012.10.043
  • Peer Reviewed
• [Journal Article] Lactococcus lactis subsp. cremoris FC triggers IFN-γproduction from NK and T cells via IL-12 and IL-18. (2012)
  • Author(s): A. Kosaka, H. Yan, S. Ohashi, Y. Gotoh, A. Sato, H. Tsutsui, T. Kaisho, T. Toda, Tsuji NM.
  • Journal Title: Int Immunopharmacol. Volume: 14(4) Pages: 729-33
  • DOI: doi:10.1016/j.intimp.2012.10.007
  • Peer Reviewed
• [Journal Article] Treml4, an Ig Superfamily Member, Mediates Presentation of Several Antigens to T Cells In Vivo, Including Protective Immunity to HER2 Protein. (2012)
  • Author(s): H. Hemmi, N. Zaidi, B. Wang, I. Matos, C. Fiorese, A. Lubkin, L. Zbytnuik, K. Suda, K. Zhang, M. Noda, T. Kaisho, R. M. Steinman, J. Idoyaga
  • Journal Title: J. Immunol. Volume: 188 Pages: 1147-1155
  • DOI: doi:10.4049/jimmunol.1102541
  • Peer Reviewed
• [Journal Article] Differential MyD88/IRAK4 requirements for cross-priming and tumor rejection induced by heat shock protein 70-model antigen fusion protein. (2012)
  • Author(s): S. Mizukami, C. Kajiwara, M. Tanaka, T. Kaisho, H. Udono.
  • Journal Title: Cancer Sci. Volume: 103(5) Pages: 851-9
  • DOI: doi:10.1111/j.1349-7006.2
  • Peer Reviewed
• [Journal Article] The Ets transcription factor Spi-B is essential for the differentiation of intestinal microfold cells. (2012)
  • Author(s): T. Kanaya, K. Hase, D. Takahashi, S. Fukuda, K. Hoshino, I. Sasaki, H. Hemmi, K.A. Knoop, N. Kumar, M. Sato, T. Katsuno, O. Yokosuka, K. Toyooka, K. Nakai, A. Sakamoto, Y. Kitahara, T. Jinnohara, S.J. McSorley, T. Kaisho, I.R. Williams, H. Ohno.
  • Journal Title: Nat Immunol. Volume: 13(8) Pages: 729-36
  • DOI: doi:10.1038/ni.2352
  • Peer Reviewed
• [Journal Article] Spi-B is critical for plasmacytoid dendritic cell function and development. (2012)
  • Author(s): I. Sasaki, K. Hoshino, T. Sugiyama, C. Yamazaki, T. Yano, A. Iizuka, H. Hemmi, T. Tanaka, M. Saito, M. Sugiyama, Y. Fukuda, T. Ohta, K. Sato, A. Ainai, T. Suzuki, H. Hasegawa, N. Toyama -Sorimachi, H. Kohara, T. Nagasawa, T. Kaisho.
  • Journal Title: Blood Volume: 120 Pages: 4733-4743
  • DOI: doi:10.1182/blood-2012-06-436527
  • Peer Reviewed
• [Journal Article] 樹状細胞サブセットの機能と生理的意義. (2012)
  • Author(s): 佐々木泉、改正恒康
  • Journal Title: 炎症と免疫 Volume: 20(1) Pages: 21-26
• [Journal Article] CD8陽性樹状細胞サブセットの動的制御機構の解析 (2012)
  • Author(s): 邊見弘明、山崎千尋、星野克明、改正恒康
  • Journal Title: A chemokine system in CD8 positive dendritic cells. Cytometry Research Volume: 22(1) Pages: 31-35
• [Journal Article] PDLIM2 inhibits T helper cell development and granulomatous inflammation through degradation of STAT3 (2011)
  • Author(s): T. Tanaka, Y. Yamamoto, R. Muromoto, O. Ikeda, Y. Sekine, M. J. Grusby, T. Kaisho, T. Matsuda.
  • Journal Title: Sci. Signal. Volume: 4(202) Pages: ra85
  • DOI: doi:10.1126/scisignal.2001637
  • Peer Reviewed
• [Journal Article] Plasmacytoid dendritic cells are crucial for the initiation of inflammation and T cell immunity in vivo (2011)
  • Author(s): H. Takagi, T. Fukaya, K. Eizumi, Y. Sato, K. Sato, A. Shibazaki, H. Otsuka, A. Hijikata, T. Watanabe, O. Ohara, T. Kaisho, B. Malissen, K. Sato.
  • Journal Title: Immunity Volume: 35 Pages: 958-971
  • DOI: doi:10.1016/j.immuni.2011.10.014
  • Peer Reviewed
• [Journal Article] 免疫アジュバントの特性とTh 細胞分化誘導. New insights into immune adjuvants and induction of Th cell differentiation (2011)
  • Author(s): 大田友和、改正恒康
  • Journal Title: 臨床免疫・アレルギー科 Clinicl Immunology & Allergology Volume: 56(5) Pages: 558-563
• [Journal Article] 樹状細胞サブセット機能を制御する分子機構. (2011)
  • Author(s): 改正恒康
  • Journal Title: アレルギー Volume: 60(5) Pages: 559-565
• [Presentation] The crucial role of Spi-B transcription factor in M-cell differentiation. (2013)
  • Author(s): T. Kanaya, K. Hase, T. Kaisho, I.R.Williams, H.Ohno.
  • Organizer: The 16th International Congress of Mucosal Immunology.
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 20130717-20
• [Presentation] Visualization of Inflammasome activation: monitoring caspase-1 activity in living cells by a genetically-encoded probe SCAT1 (2012)
  • Author(s): T. Liu, Y. Yamaguchi, K. Hoshino, T. Kaisho, K. Takemoto, E. Kuranaga, M. Miura
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: マリンメッセ福岡
  • Year and Date: 20121211-14
• [Presentation] The Ets transcription factor Spi-B is essential for M-cell differentiation (2012)
  • Author(s): T. Kanaya, K. Hase, T. Kaisho, R. W. Ifor, H Ohno
  • Organizer: 第41回日本免疫学会総会・学術集会記録 41:210,2012
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 20121205-07
• [Presentation] Spi-B is critical for plasmacytoid dendric cell development in bone marrow. (2012)
  • Author(s): I. Sasaki, K. Hoshino, H. Hemmi, M. Sugiyama, T. Yamaguchi, T. Kaisho
  • Organizer: 第41回日本免疫学会総会・学術集会記録 41:162,2012
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 20121205-07
• [Presentation] Analysis of in vivo function of XC chemokine receptor-expressing dendric cells. (2012)
  • Author(s): M. Sugiyama, H. Hemmi, C. Yamazaki, T. Ohta, I. Sasaki, K. Hoshino, T. Kaisho
  • Organizer: 第41回日本免疫学会総会・学術集会記録41:158, 2012
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 20121205-07
• [Presentation] Long-term memory T cell response induced by adjuvant role of iNKT cells. (2012)
  • Author(s): K. Shimizu, M. Asakura, J. Shinga, Y. Sato, K. Hoshino, T.Kaisho, T. Ezaki, S. Fujii.
  • Organizer: 第41回日本免疫学会総会・学術集会記録41:133,2012
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 20121205-07
• [Presentation] IL-27 affects immune responses via regulation of prostaglandin E2 production by macrophages. (2012)
  • Author(s): Y. Sato, S. Suzuki, H. Hara, T. Kaisho, H. Yoshida
  • Organizer: 第41回日本免疫学会総会・学術集会記録41:92,2012
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 20121205-07
• [Presentation] A non-synonymous SNP(single nucleotide polymorphism) of PDLIM4 is associated with susceptibility of rheumatoid arthritis and Graves diseases. (2012)
  • Author(s): T. Tanaka, Y. Kochi, K. Yamamoto, T. Kaisho
  • Organizer: 第41回日本免疫学会総会・学術集会記録41:74, 2012
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 20121205-07
• [Presentation] -deficiency in Epithelial Cells Elicits Sjogren's Syndrome-like Autoimmune Disease. (2012)
  • Author(s): A. Okuma, K. Hoshino, T.Kaisho, T. Muta
  • Organizer: 第41回日本免疫学会総会・学術集会記録 41:33,2012
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 20121205-07
• [Presentation] I B --deficient Mice Develop Sjogren's Syndrome-like Chronic Inflammation Caused by Dysregulation of Epithelial Homeostasis. (2012)
  • Author(s): A. Okuma, K. Hoshino, T. Kaisho, T. Muta
  • Organizer: IEIIS2012 Homeostatic Inflammation Symposium
  • Place of Presentation: Tokyo, Japan.
  • Year and Date: 20121023-26
• [Presentation] Roles of an Ets family transcription factor, Spi-B, in plasmacytoid dendritic cell development in the bone marrow. (2012)
  • Author(s): I. Sasaki, K. Hoshino, H. Hemmi, M.Sugiyama, T. Yamaguchi, T. Kaisho
  • Organizer: IEIIS2012 Homeostatic Inflammation Symposium
  • Place of Presentation: Tokyo, Japan.
  • Year and Date: 20121023-26
• [Presentation] In vivo roles of XC chemokine receptor 1-expressing dendritic cells. (2012)
  • Author(s): M. Sugiyama, H. Hemmi, C. Yamazaki, T.Ohta, I. Sasaki, K. Hoshino, T. Kaisho
  • Organizer: IEIIS2012 Homeostatic Inflammation Symposium
  • Place of Presentation: Tokyo, Japan.
  • Year and Date: 20121023-26
• [Presentation] Function and differentiation of M cells, a unique subset of intestinal epithelial cells specialized for mucosal antigen-uptake (2012)
  • Author(s): T. Kanaya, K. Hase, T Kaisho, I. Williams, H. Ohno.
  • Organizer: DC2012
  • Place of Presentation: Daege, Korea
  • Year and Date: 20121007-11
• [Presentation] Plasmacytoid dendritic cells are crucial for the initiation of inflammation and T cell immunity in vivo (2012)
  • Author(s): H. Takagi, R. Murakami, T. Fukaya, O. Ohara, T. Kaisho, B. Malissen, K. Sato
  • Organizer: The 12th International Symposium on Dendritic Cells, DC 2012
  • Place of Presentation: Daegu, Korea
  • Year and Date: 20121007-11
• [Presentation] Ablation of a dendritic cell subset expressing XC chemokine receptor 1 in vivo. (2012)
  • Author(s): H. Hemmi, C. Yamazaki, M. Sugiyama, T.Ohta, I. Sasaki, K. Hoshino, T. Kaisho
  • Organizer: The 12th International Symposium on Dendritic Cells, DC 2012
  • Place of Presentation: Daegu, Korea
  • Year and Date: 20121007-11
• [Presentation] Critical role of Siglec-H in the function of plasmacytoid dendritic cells for the regulation of inflammation and T cell immunity in vivo (2012)
  • Author(s): R. Murakami, H. Takagi, T. Fukaya, O. Ohara, T. Kaisho, B. Malissen, K. Sato.
  • Organizer: The 12th International Symposium on Dendritic Cells, DC 2012
  • Place of Presentation: Daegu, Korea
  • Year and Date: 20121007-11
• [Presentation] M cells, a unique subset of intestinal epithelial cells specialized for mucosal antigen-uptake. (2012)
  • Author(s): T. Kanaya, K. Hase, T Kaisho, I. Williams, H. Ohno
  • Organizer: The Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji Island, Hyogo
  • Year and Date: 20120811-14
• [Presentation] In vivo ablation of XC chemokine receptor 1-expressing dendritic cell subset. (2012)
  • Author(s): M. Sugiyama, H. Hemmi, C. Yamazaki, I. Sasaki, T. Ohta, K. Hoshino, T. Kaisho.
  • Organizer: 20th International Symposium on Molecular Cell Biology of Macrophages 2012
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20120615-16
• [Presentation] サイエンスカフェ免疫研究の最前線 (2012)
  • Author(s): 改正恒
  • Organizer: 樹状細胞は免疫のキープレーヤー
  • Place of Presentation: 大阪
  • Year and Date: 2012-04-30
• [Presentation] 樹状細胞サブセット機能を制御する分子機構 (2012)
  • Author(s): 改正恒
  • Place of Presentation: 大阪市立大学(大阪)
  • Year and Date: 2012-01-12
• [Presentation] Evaluation of SCAT1: a genetically-encoded probe for detecting activation of caspase-1 in living cells. (2011)
  • Author(s): K. Shikada, Y. Yamaguchi, T. Liu, K. Takemoto, K. Hoshino, T. Kaisho. E. Kuranaga, M. Miura.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 20111213-16
• [Presentation] HSP70 is essential for PDLIM2-mediated termination of NF-・B signaling. (2011)
  • Author(s): T. Tanaka, T. Kaisho.
  • Organizer: 第40回日本免疫学会総会・学術集会記録 40:36, 2011
  • Place of Presentation: 幕張メッセ
  • Year and Date: 20111127-29
• [Presentation] PDLIM4 negatively regulates STAT signaling by recruiting protein tyrosine phosphatases. (2011)
  • Author(s): T. Hirashima, T. Kaisho, T. Tanaka.
  • Organizer: 第40回日本免疫学会総会・学術集会記録 40:109, 2011
  • Place of Presentation: 幕張メッセ
  • Year and Date: 20111127-29
• [Presentation] Crucial role of plasmacytoid dendritic cells in the regulation of inflammation and T Cell immunity in vivo. (2011)
  • Author(s): H. Takagi, T. Fukaya, Y. Sato, K. Sato, A. Shibazaki, A. Hijikata, O. Ohara, T Kaisho, B. Malissen, K. Sato.
  • Organizer: 第40回日本免疫学会総会・学術集会記録40:118, 2011
  • Place of Presentation: 幕張メッセ
  • Year and Date: 20111127-29
• [Presentation] B. Malissen. Critical role of siglec-H in the function of plasmacytoid dendritic cells for the regulation of inflammation and T Cell immunity in vivo (2011)
  • Author(s): K. Sato, H. Takagi, T. Fukaya, Y. Sato, K. Sato, A. Shibazaki, A. Hijikata, O. Ohara, T Kaisho
  • Organizer: 第40回日本免疫学会総会・学術集会記録 40:118, 2011
  • Place of Presentation: 幕張メッセ
  • Year and Date: 20111127-29
• [Presentation] Critical roles of murine XC chemokine receptor 1-expressing DC subset in vivo. (2011)
  • Author(s): C. Yamazaki, H. Hemmi, R. Miyamoto, I. Sasaki, T. Ito, K. Hoshino, T. Kaisho
  • Organizer: 第40回日本免疫学会総会・学術集会記録 40:119, 2011
  • Place of Presentation: 幕張メッセ
  • Year and Date: 20111127-29
• [Presentation] Double-stranded RNA of lactic acid bacteria triggers TLR3-mediated IFN-・production by dendritic cells. (2011)
  • Author(s): T. Kawashima, H. Yan, Z. Guo, R. Uchiyama, J. Carreras, T. Kaisho, O. Takeuchi, S. Akira, H. Tsutsui, T. Saito, I. Nishimura, N. Tsuji
  • Organizer: 第40回日本免疫学会総会・学術集会記録 40:121, 2011
  • Place of Presentation: 幕張メッセ
  • Year and Date: 20111127-29
• [Presentation] Importance of chemokines system between human BDCA3+ dendritic cells and NK cells in innate cytotoxic response (2011)
  • Author(s): T. Ito, R. Miyamoto, C. Yamazaki, K. Hoshino, T. Kaisho, S. Nomura.
  • Organizer: 第40回日本免疫学会総会・学術集会記録 40:197, 2011
  • Place of Presentation: 幕張メッセ
  • Year and Date: 20111127-29
• [Presentation] 樹状細胞サブセット機能を制御する分子機構 (2011)
  • Author(s): 改正恒
  • Organizer: 鳥取大学生命科学科講義
  • Place of Presentation: 米子
  • Year and Date: 2011-12-07
• [Presentation] ショートトーク自然免疫～感染の検知システム (2011)
  • Author(s): 改正恒
  • Organizer: 免疫ふしぎ未来
  • Place of Presentation: 東京
  • Year and Date: 2011-08-21
• [Presentation] 樹状細胞機能制御の分子基盤 (2011)
  • Author(s): 改正恒
  • Organizer: 免疫サマースクール
  • Place of Presentation: 宮城
  • Year and Date: 2011-08-01
• [Presentation] Dendritic cell sensing and responses for nucleic acid adjuvants. (2011)
  • Author(s): T. Kaisho
  • Organizer: KSBMB 2011 Annual Meeting
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2011-05-18
• [Presentation] Dendritic cell sensing and responses for nucleic acid adjuvants (2011)
  • Author(s): T. Kaisho.
  • Organizer: IVI seminar
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2011-05-17
• [Presentation] 核酸系免疫アジュバントに対する樹状細胞サブセットの応答機構 (2011)
  • Author(s): 改正恒康
  • Organizer: 第29回日本耳鼻咽喉科免疫アレルギー学会
  • Place of Presentation: 大分
  • Year and Date: 2011-02-10
• [Presentation] Dendritic cell subsets in inflammation. (2010)
  • Author(s): T. Kaisho
  • Organizer: The 12th Biennial International Endotoxin & Innate Immunity Society (IEIIS) Meeting
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20101023-26
• [Presentation] The role of M cells in intestinal immunity. (2010)
  • Author(s): T. Kanaya, K. Hase, G. Nakato, T. Kaisho, I. R. Williams, H. Ohno
  • Organizer: The 12th Biennial International Endotoxin & Innate Immunity Society (IEIIS) Meeting
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20101023-26
• [Book] 樹状細胞の免疫調節機構. In 免疫学Update 分子病態の解明と治療への展開- (2012)
  • Author(s): 邊見弘明、改正恒康
  • Total Pages: 98-106
  • Publisher: 南山堂
• [Book] I型インターフェロン. I nサイトカインのすべて(完全改訂版) (2012)
  • Author(s): 改正恒康
  • Total Pages: 243-249
  • Publisher: 化学評論社
• [Book] 樹状細胞機能を制御する分子基盤 In アジュバント開発研究の新展開 (2011)
  • Author(s): 改正恒康
  • Total Pages: 143-150
  • Publisher: シーエムシー出版
• [Book] Principles of innate immunity, in Fifth Edition Rheumatology vol.1 (edited by Marc C. Hochberg, Alan J. Silman, Josef S. Smolen, Michael E. Weinblatt. Michael H. Weisman). (2011)
  • Author(s): T. Kaisho and S. Akira.
  • Total Pages: 141-151
  • Publisher: MOSBY, ELSEVIER, USA
• [Remarks]
  • URL: http://immreg.ifrec.osaka-u.ac.jp/www/publication/
• [Remarks]
  • URL: http://www.osaka-u.ac.jp/ja/news/ResearchRelease/2012/06/20120618_1