2012 Fiscal Year Final Research Report
Property investigation of circulating tumor cells, primary and metastatic cancer tissues by use of whole genome sequencing and application to laboratory testing
| Project/Area Number |
23659295
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Yoshihisa 浜松医科大学, 医学部, 助教 (00362187)
SETOU Mitsutoshi 浜松医科大学, 医学部, 教授 (20302664)
NAKAMURA Toshio 浜松医科大学, 医学部, 准教授 (40283353)
SUGIMURA Haruhiko 浜松医科大学, 医学部, 教授 (00196742)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 循環腫瘍細胞 / オミックス解析 / リピドミクス / 次世代シーケンサーーケンサー |
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| Research Abstract |
We investigated primary focus, metastatic focus and circulating tumor cells (CTC) in colorectal cancer (CRC) patients by genomics approach in order to deve lop a novel clinical laboratory testing. CRC cell lines were mixed with peripheral blood from healthy volunteer, and isolated from the mixture. Captured by magnetic cell separation and flow cytometry, the cancer cell lines were collected and confirmed by immunostaining with anti-cytokeratin antibody. The technologies were established, however, CTC could not be isolated from CRC patients in early stage. We implemented iTRAQ (isobaric tags for relative and absolute quantitation) method to develop a novel CRC biomarker, and found LRPPRC (Leucine rich PPR motif containing protein) that was expressed 1.62 fold higher in cancer tissues. Tissue-Microarray and immunohistochemistry revealed that the expression level of LRPPRC was related to cancer differentiation. LRPPRC could be a novel marker for prognosis prediction.
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[Journal Article] Plasma CCN2 (connective tissue growth factor ; CTGF) is a potential biomarker in idiopathic pulmonary fibrosis (IPF).2012
Author(s)
Kono M, Nakamura Y, Suda T, Kato M,Kaida Y, Hashimoto D,Inui N, Hamada E,Miyazaki O,Kurashita S, Fukamachi I, Endo K, Ng P, Takehara K, Nakamura H,Maekawa M, Chida K
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Journal Title
Clinica Chimica Acta
Volume: 412
Pages: 2211-2215
DOI
Peer Reviewed
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[Journal Article] Hepatocellular adenoma associated with familial adenomatous polyposis coli2012
Author(s)
Inaba K, Sakaguchi T, Kurachi K, Mori H, Tao, H, Nakamura T, Takehara Y, Baba S, Maekawa M, Sugimura H, Konno H
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Journal Title
World Journal of Hepatology
Volume: 4(11)
Pages: 322-326
DOI
Peer Reviewed
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