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2012 Fiscal Year Final Research Report

Development of diagnosis test kits with high specificity, high sensitivity, and low cost against tropical infectious diseases.

Research Project

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Project/Area Number 23659297
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Laboratory medicine
Research InstitutionOsaka University

Principal Investigator

IKUTA Kazuyoshi  大阪大学, 微生物病研究所, 教授 (60127181)

Co-Investigator(Kenkyū-buntansha) KUROSU Takeshi  大阪大学, 微生物病研究所, 助教 (70432432)
SAKUDO Akikazu  琉球大学, 医学部保健学科, 准教授 (10397672)
Research Collaborator OKABAYASHI Tamaki  大阪大学, 微生物病研究所, 特任准教授 (10359995)
SASAKI Tadahiro  大阪大学, 微生物病研究所, 特任講師 (20547533)
KOSAKA Mieko  アルフレッサファーマ株式会社, 診断薬研究開発部, 室長 (70592278)
NAKAISHI Kazunari  株式会社 BL, 商品開発部, 部長
Project Period (FY) 2011 – 2012
Keywords臨床微生物学 / 迅速診断
Research Abstract

Tropical infections are especially endemic in low-income populations in developing regions. Dengue disease that is induced by dengue virus is difficult for differential diagnosis from other fabric infectious diseases. The aim of this study, we developed immune-chromatograph rapid test kit against dengue, chikungunya, and influenza virus infections with high sensitivity, high specificity, low cost, and simple use at clinics.

  • Research Products

    (10 results)

All 2013 2012 2011 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (1 results) Remarks (2 results)

  • [Journal Article] Characterization of chikungunya virus infection of a human keratinocyte cell line: Role of mosquito salivary gland protein in suppressing the host immune response.2013

    • Author(s)
      Puiprom O, Morales Vargas RE, Potiwat R, Chaichana P, Ikuta K, Ramasoota P, Okabayashi T.
    • Journal Title

      Infect Genet Evol

      Volume: 17C Pages: 210-215

    • DOI

      doi:10.1016/j.meegid.2013.04.005

    • Peer Reviewed
  • [Journal Article] Dengue virus neutralization and antibody-dependent enhancement activities of human monoclonal antibodies derived from dengue patients at acute phase of secondary infection.2013

    • Author(s)
      Sasaki T, Setthapramote C, Kurosu T, Nishimura M, Asai A, Omokoko MD, Pipattanaboon C, Pitaksajjakul P, Limkittikul K, Subchareon A, Chaichana P, Okabayashi T, Hirai I, Leaungwutiwong P, Misaki R, Fujiyama K, Ono K, Okuno Y, Ramasoota P, Ikuta K.
    • Journal Title

      Antiviral Res.

      Volume: 98(3) Pages: 423-31

    • DOI

      doi:10.1016/j.antiviral.2013.03.018

    • Peer Reviewed
  • [Journal Article] Reliability of a newly-developed immunochromatography diagnostic kit for pandemic influenza A/H1N1pdm virus: implications for drug administration.2012

    • Author(s)
      Sasaki T, Kubota-Koketsu R, Takei M, Hagihara T, Iwamoto S, Murao T, Sawami K, Fukae D, Nakamura M, Nagata E, Kawakami A, Mitsubayashi Y, Ohno M, Uehara Y, Fukukawa T, Kanai Y, Kosaka M, Ikuta K.
    • Journal Title

      PLoS One.

      Volume: 7 Pages: e50670

    • DOI

      doi:10.1371/journal.pone.0050670

    • Peer Reviewed
  • [Journal Article] Limited cross-reactivity of mouse monoclonal antibodies against Dengue virus capsid protein among four serotypes.2012

    • Author(s)
      Noda M, Masrinoul P, Punkum C, Pipattanaboon C, Ramasoota P, Setthapramote C, Sasaki T, Sasayama M, Yamashita A, Kurosu T, Ikuta K, Okabayashi T.
    • Journal Title

      Biologics

      Volume: 6 Pages: 409-16

    • DOI

      doi:10.2147/BTT.S37792

    • Peer Reviewed
  • [Journal Article] Human monoclonal antibodies to neutralize all dengue virus serotypes using lymphocytes from patients at acute phase of the secondary infection.2012

    • Author(s)
      Setthapramote C, Sasaki T, Puiprom O, Limkittikul K, Pitaksajjakul P, Pipattanaboon C, Sasayama M, Leuangwutiwong P, Phumratanaprapin W, Chamnachanan S, Kusolsuk T, Jittmittraphap A, Asai A, Arias JF, Hirai I, Kuhara M, Okuno Y, Kurosu T, Ramasoota P, Iku
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 423(4) Pages: 867-72

    • DOI

      doi:10.1016/j.bbrc.2012.06.057.

    • Peer Reviewed
  • [Journal Article] Development of two types of rapid diagnostic test kits to detect the hemagglutinin or nucleoprotein of the swine-origin pandemic influenza A virus H1N1.2011

    • Author(s)
      Mizuike R, Sasaki T, Baba K, Iwamoto H, Shibai Y, Kosaka M, Kubota-Koketsu R, Yang CS, Du A, Sakudo A, Tsujikawa M, Yunoki M, Ikuta K
    • Journal Title

      Clin Vaccine Immunol

      Volume: 18(3) Pages: 494-9

    • DOI

      doi:10.1128/CVI.00269-10.

    • Peer Reviewed
  • [Journal Article] Co-existence of major and minor viral populations from two different origins in patients secondarily infected with dengue virus serotype 2 in Bangkok2011

    • Author(s)
      Puiprom O, Yamashita A, Sasayama M, Limkittikul K, Boonha K, Jittmitraphap A, Leaungwutiwong P, Kurosu T, Ramasoota P, Ikuta K.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 413(1) Pages: 136-42

    • DOI

      doi:10.1016/j.bbrc.2011.08.069.

    • Peer Reviewed
  • [Presentation] シーズンの大阪府下におけるインフルエンザ様疾患患者検体を用いた Pandemic A(H1N1)2009迅速診断キットの評価2012

    • Author(s)
      佐々木正大
    • Organizer
      第53回日本臨床ウイルス学会
    • Place of Presentation
      大阪
    • Year and Date
      2012-06-17
  • [Remarks]

    • URL

      http://virology.biken.osaka-u.ac.jp/

  • [Remarks] マヒドン大学における医療関係者への迅速診断キットのセミナー (2011年7月27日)

URL: 

Published: 2014-09-25  

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