2013 Fiscal Year Final Research Report
Novel approach for discovery of biomarkers by comprehensive analysis of immune complexes
| Project/Area Number |
23659301
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Nagasaki University |
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Principal Investigator |
KURODA Naotaka 長崎大学, 医歯(薬)学総合研究科, 教授 (50234612)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 臨床化学 |
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| Research Abstract |
We proposed a novel proteomic strategy (immune complexome analysis) consisting of ICs separation from serum and direct tryptic digestion followed by nano-liquid chromatography-tandem mass spectrometry for the identification and profiling of antigens in circulating immune complexes (CICs). We applied this strategy to the analysis of CICs in rheumatoid arthritis (RA). Sera samples from healthy donors and osteoarthritis patients were used as controls. CIC-associated thrombospondin-1 (TSP-1) was found in 81%, and CIC-associated platelet factor 4 (PF4) was found in 52% of established RA patients, but neither protein was found in CICs from any of the controls. Furthermore, CIC-associated TSP-1 was found only in early RA patients and was found in neither disease controls nor healthy donors. It was also found that seronegative early RA patients, who lack lacking RF and anti-CCP antibody markers, had CIC-associated TSP-1.
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