2012 Fiscal Year Final Research Report
Classification of hematologic malignancy by relationship between cancer and expression of human cellular phosphoprotein.
| Project/Area Number |
23659304
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Bunkyo Gakuin University |
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Principal Investigator |
MOTOFUJI Yoko 文京学院大学, 保健医療技術学部, 助手 (00458556)
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| Co-Investigator(Renkei-kenkyūsha) |
KOMATSU Hiroyoshi 文京学院大学, 大学院・保健医療科学研究科, 教授 (30170377)
KOIKE Morio 文京学院大学, 大学院・保健医療科学研究科, 教授 (00089989)
KODERA Yoshio 北里大学, 大学院・理学研究科, 准教授 (60265733)
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| Project Period (FY) |
2011 – 2012
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| Keywords | モノクローナル抗体 / リン酸化蛋白質 / 腫瘍検査 / プロテオーム / 血液悪性腫瘍 |
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| Research Abstract |
On the basis of the reaction of the human leukemia cell lines with anti - phosphoprotein monoclonal antibodies, the reactivity of anti - phosphoprotein monoclonal antibodies was in vestigated using cells of hematologic malignancy. The 110 anti-phosphoprotein monoclonal antibodiesreacted with reactivity to formalin-fi xedtissuesandcells . A part of them had tumor specificity by immunohistochemical staining; the specificity of anti - phosphoprotein monoclonal antibody was identified by proteomics. To investigate the relationship between antigen molecule and leukemia c lassification, the effect of staining in hematology and of anticancer agents towards leukemia cell lines were examined . As he results , the antigen molecules anti - phosphoprotein monoclonal antibodies recognized related to the response of the anticancer agents towards T cell leukemia.
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