Analysis of novel autoantigens with labile epitopes on cell surface, and development of new diagnostic test for the detection of autoantibodies

2014 Fiscal Year Final Research Report

• Project/Area Number: 23659312
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Fujita Health University
• Principal Investigator: MIURA Keiji 藤田保健衛生大学, 医療科学部, 講師 (20199946)
• Co-Investigator(Renkei-kenkyūsha): YOSHIDA Shunji 藤田保健衛生大学, 医学部, 教授 (00166951)
• Research Collaborator: TAKAHASHI Kazuo 藤田保健衛生大学, 医学部, 講師 (90631391) ; IWATA Yohei 藤田保健衛生大学, 医学部, 講師 (60437861)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: 自己免疫疾患 / 自己抗体 / 自己抗原 / 血管内皮細胞 / 細胞表面抗原 / 膜タンパク / 診断法

Outline of Final Research Achievements

We developed a new assay for detecting AECA by CSP-ELISA. IgG-AECA positive rates of using CSP-ELISA were 95% for SLE, 97% for MCTD and 58% for SSc. Also, IgA-AECA positively rates were 34%, 97%, and 58% respectively. AECA in patients of these disorders recognized membrane proteins expressed on cell surface of endothelial cells. CSP-ELISA holds promise for improving the detection of anti-cell surface autoantibodies and the diagnosis in the clinical context.

Free Research Field

臨床免疫学