Development of a novel method for high-throughput discovery of trans- spliced RNAs in mammalian cells

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659313
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Kurume University
• Principal Investigator: IMAMURA Rie 久留米大学, 医学部, 助教 (70309764)
• Project Period (FY): 2011 – 2012
• Keywords: 遺伝子検査学

Research Abstract

Wedevelopedanovelmethodforhigh-throughputdiscoveryoftrans-splicedRNAsinmammalian cells. Our method is based on a mate-pair analysis, which enables us to collect information of both ends of DNA fragments. In conventional mate-pair analysis, there must contain circularized DNAs derived from multiple DNAs and those mate-pair fragments can be a cause of false positive trans-spliced genes. To solve this problem we developed a novel method, which allows us to prepare circularized DNA libraries in which each circularized DNA is originated from each single DNA. By this method, we can make a systematic survey of the presence of trans-spliced RNAs in mammalian cells in a highly precise manner, and we could find out several candidate genes in hematopoietic stem cells. This method is useful for discovery of trans-spliced RNAs and will enable us to understand the role of trans-spliced RNAs in mammalian.

Research Products

• [Journal Article] Successful treatment of small cell variant anaplastic large cell lymphoma with allogeneic peripheral blood stem cell transplantation, and review of the literature. (2013)
  • Author(s): Imamura R,Mouri F, Nomura K,NakamuraT, Oku E, Morishige S,Takata Y, Seki R, Osaki K, Hashiguchi M, Yoshimoto K, Ohshima K, Nagafuji K, Okamura T.
  • Journal Title: Int J Hematol. Pages: 139-143
  • DOI: doi10.1007/s12185-012-1242-3
  • Peer Reviewed
• [Journal Article] A case of 8p11 myeloproliferative syndrome withBCR- FGFR1 gene fusion presenting with trilineage acute leukemia/lymphoma, successfully treated by cord blood transplantation. (2013)
  • Author(s): Morishige S, Oku E, Takata Y, Kimura Y, Arakawa F, Seki R, Imamura R, Osaki K, Hashiguchi M, Yakushiji K, Mizuno S, Yoshimoto K, Nagafuji K, Ohshima K, Okamura T.
  • Journal Title: Acta Haematol Volume: 129 Pages: 83-89
  • DOI: doi:10.1159/000341289
  • Peer Reviewed
• [Journal Article] Unrelated cord blood transplantation for patients with adult T-cell leukemia/lymphoma: experience at a single institute. (2012)
  • Author(s): Nakamura T, Oku E, Nomura K, Morishige S, Takata Y, Seki R, Imamura R, Osaki K, Hashiguchi M, Yakushiji K, Mouri F, Mizuno S, Yoshimoto K, Ohshima K, Nagafuji K, Okamura T.
  • Journal Title: Int J Hematol. Volume: 96 Pages: 657-663
  • DOI: doi:10.1007/s12185-012-1177-8
  • Peer Reviewed
• [Journal Article] Amebic colitis and liver abscess complicated by high serum procalcitonin in acute myeloid leukemia. (2012)
  • Author(s): Oku E, Nomura K, Nakamura T, Morishige S, Seki R, Imamura R, Hashiguchi M, Osaki K, Mizuno S, Nagafuji K, Okamura T.
  • Journal Title: Kansenshogaku Zasshi. Volume: 86 Pages: 773-777
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/23367854
  • Peer Reviewed