2013 Fiscal Year Final Research Report
Developing the high sensitive immunochromatograhy with a newl method for improving immunofluorescent signals
| Project/Area Number |
23659314
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Tokyo Metropolitan Institute of Medical Science |
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Principal Investigator |
SHIBASAKI Futoshi 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 参事研究員 (90300954)
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| Co-Investigator(Kenkyū-buntansha) |
SAKURAI Akira 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 研究員 (40546628)
ENDO Fumiko 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 研究員 (80546630)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 診断 / 蛍光 / イムノクロマト / インフルエンザ / 子宮頚癌 |
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| Research Abstract |
In this study, we focused on the specific aim to develop the fluorescent enhancement methods (IMPACTag) for improving the MUSTag sensitivity and also apply this methods to Immunochromatography and the devices.
First, we tried to develop the IMPACTag, but the sensitivity was not enough to our goal, and we changed the strategy to use fluorescent beads instead of IMPACTag. During our research term, we succeeded in developing the high sensitive immunochromatography (IC) tips and the device in collaboration with companies. For detection of influenza viruses, the IC chip and the device could detect viruses 100 fold more sensitive than conventional methods. Furthermore, we also succeeded in the developing the assay system for detecting antibodies against papilloma virus L1 proteins. the system will be available for the evaluation of the cervical cancer vaccine effect after the inoculation.
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[Journal Article] Histopathological Evaluation of the Diversity of Cells Susceptible to H5N1 Virulent Avian Influenza Virus2013
Author(s)
H. Ogiwara, F. Yasui, K. Munekata, A. Takagi-Kamiya, T. Munakata, N. Nomura, F. Shibasaki, K. Kuwahara, N. Sakaguchi, Y. Sakoda, H. Kida and M. Kohara
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Journal Title
Am J Pathol
Volume: S0002-9440(13)
Pages: 00681-0
Peer Reviewed
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[Journal Article] Broad-Spectrum Detection of H5 Subtype Influenza A Viruses with a New Fluorescent Immunochromatography System2013
Author(s)
Akira Sakurai, Katsuyoshi Takayama, Namiko Nomura, Tsubasa Munakata, Naoki Yamamotod, Tsuruki Tamura, Jitsuho Yamada, Masako Hashimoto, Kazuhiko Kuwahara, Yoshihiro Sakoda, Yoshihiko Suda, Yukuharu Kobayashi, Nobuo Sakaguchi, Hiroshi Kida, Michinori Kohara, Futoshi Shibasaki
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Journal Title
PLoS ONE
Volume: 8(11)
Pages: e76753
Peer Reviewed
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[Journal Article] Rapid typing of influenza viruses using super high-speed quantitative real-time PCR2011
Author(s)
Sakurai A, Nomura N, Nanba R, Sinkai T, Iwaki T, Obayashi T, Hashimoto K, Hasegawa M, Sakoda Y, Naito A, Morizane Y, Hosaka M, Tsuboi K, Kida H, Kai A, Shibasaki F
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Journal Title
J Virol Methods
Volume: 178(1-2)
Pages: 75-81
Peer Reviewed
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