2013 Fiscal Year Final Research Report
Study on gate control theory of pain by two-photon microsucopy using gene-engineered mice.
| Project/Area Number |
23659322
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pain science
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| Research Institution | Kansai Medical University |
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Principal Investigator |
ITO Seiji 関西医科大学, 医学部, 教授 (80201325)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMURA Shinji 関西医科大学, 医学部, 講師 (70276393)
NISHIDA Kazuhiko 関西医科大学, 医学部, 助教 (80448026)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKATSUKA Terumasa 関西医療大学, 保健医療学部, 教授 (30380752)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 多光子励起顕微鏡 / 脊髄後角 / 一次求心性線維 / ゲートコントロール説 / in vivoイメージング / トランスジェニックマウス / 痛覚 / 神経回路網 |
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| Research Abstract |
Peripheral stimuli in the skin are selected in the dorsal horn of spinal cord, and noxious stimuli are recognized as pain in the brain. In order to clarify the selection mechanism in the spinal cord, we established the imaging system for the spinal network which can chase for a long time in vivo using two-photon microscopy and gene-engineered mice expressing fluorescent protein. In this study, we demonstrated that intensity of neurites and puncta in the spinal cord increased for 30 min to 4 hours after injection of formalin in the foot, and that a network of neurons which respond to mechanical, touch, and thermal stimuli to the skin was visualized in vivo in fluorescent protein-expressing mice.
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[Journal Article] The action site of the synthetic kainoid (2S,3R,4R)-3-carboxymethyl-4-(4-methylphenylthio) pyrrolidine-2-carboxylic acid (PSPA-4), an analogue of Japanese mushroom poison acromelic acid, for allodynia (tactile pain)2013
Author(s)
Miyazaki, S., Minami, T., Mizuma, H., Kanazawa, M., Doi, H., Matsumura, S., Lu, J., Onoe, H., Furuta, K., Suzuki, M. and Ito, S
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Journal Title
Eur. J. Pharmacol
Volume: 710
Pages: 120-127
DOI
Peer Reviewed
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[Journal Article] Involvement of Tyr1472 phosphorylation of NMDA receptor NR2B subunit in postherpetic neuralgia in model mice2012
Author(s)
Unezaki, S., Sasaki, A., Mabuchi, T., Matsumura, S., Katano, T., Nishio, N., Andoh, T., Nakazawa, T., Yamamoto, T., Nakatsuka, T., Kuraishi, Y. and Ito, S
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Journal Title
Mol. Pain
Volume: 8
Pages: 59(1-13)
DOI
Peer Reviewed
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[Journal Article] Identification of NIPSNAP1 as a nocistatin-interacting protein involving pain transmission2012
Author(s)
Okuda-Ashitaka, E., Minami, T., Tsubouchi, S., Kiyonari, H., Iwamatsu, A., Noda, T., Handa, H. and Ito, S
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Journal Title
J. Biol. Chem
Volume: 287
Pages: 10403-10413
DOI
Peer Reviewed
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[Journal Article] Synthesis of an acromelic acid A analog-based 11C-labeled PET tracer for exploration of the site of action of acromelic acid A in allodynia induction2011
Author(s)
Kanazawa, M., Furuta, K., Doi, H., Mori,T., Minami, T. and Ito, S
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Journal Title
Bioorg. Med. Chem. Lett
Volume: 21
Pages: 2017-2020
DOI
Peer Reviewed
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[Presentation] Characterization of postherpetic neuralgia in mice with knock-in mutation of NMDA receptor2012
Author(s)
Ito, S., Sasaki, A., Unezaki, S., Andoh, T., Matsumura, S., Katano, T., Nishio, N., Nakatsuka, T., Kuraishi, Y. and Minami, T
Organizer
14th World Congress on Pain
Place of Presentation
Milano
Year and Date
20120827-31
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