Risk assessment of fibrous and particulate matters on the basis of microRNomics

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659328
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Hygiene
• Research Institution: Mie University
• Principal Investigator: HIRAKU Yusuke 三重大学, 大学院・医学系研究科, 講師 (30324510)
• Project Period (FY): 2011 – 2012
• Keywords: 繊維・粒子状物質 / マイクロRNA / 石綿 / シリカ / 黄砂 / カーボンナノチューブ / マイクロアレイ / リスク評価

Research Abstract

Fibrous and particulate matters cause inflammation in respiratory systems, leading to carcinogenesis and fibrosis. It is necessary to establish the system to evaluate the risk of these matters. microRNA (miRNA) is expected as a potential biomarker for various human diseases. In this study, we found that the expression of some miRNAs was significantly changed in the lung of mice exposed to asbestos, Asian dust and silica. These results suggest that miRNA can be used as a biomarker to evaluate the risk of these matters.

Research Products

• [Journal Article] Nitrative DNA damage induced by multi-walled carbon nanotube via endocytosis in human lung epithelial cells (2012)
  • Author(s): Guo F, Ma N, Horibe Y, Kawanishi S, Murata M, Hiraku Y
  • Journal Title: Toxicol. Appl.Pharmacol Volume: 260 Pages: 183-192
  • DOI: DOI:10.1016/j.taap.2012.02. 010
  • Peer Reviewed
• [Journal Article] Plasma hydroxyproline, MMP-7 and collagen I as novel predictive risk markers of hepatobiliary disease-associated cholangiocarcinoma (2012)
  • Author(s): Prakobwong S, Charoensuk L, Hiraku Y, Pinlaor P, Pairojkul C, Mairiang E, Sithithaworn P, Yongvanit P, Khuntikeo N, Pinlaor S
  • Journal Title: Int. J. Cancer Volume: 131 Pages: E416-424
  • DOI: DOI:10.1002/ijc.26443
  • Peer Reviewed
• [Journal Article] Inflammation-induced protein carbonylation contributes to poor prognosis for cholangiocarcinoma (2012)
  • Author(s): Thanan R, Oikawa S, Yongvanit P, Hiraku Y, Ma N, Pinlaor S, Pairojkul C, Wongkham C, Sripa B, Khuntikeo N, Kawanishi S, Murata M
  • Journal Title: Free. Radic. Biol. Med Volume: 52 Pages: 1465-1472
  • DOI: DOI: 10.1016/j. freeradbiomed.2012.01.018
  • Peer Reviewed
• [Journal Article] Promoter hypermethylation of Ras-related GTPase gene RRAD inactivates a tumor suppressor function in nasopharyngeal carcinoma (2012)
  • Author(s): Mo Y, Midorikawa K, Zhang Z, Zhou X, Ma N, Huang G, Hiraku Y, Oikawa S, Murata M
  • Journal Title: Cancer Lett Volume: 323 Pages: 147-154
  • DOI: DOI:10.1016/j.canlet.2012.03.042
  • Peer Reviewed
• [Journal Article] Curcumin decreases cholangiocarcinogenesis in hamsters by suppressing inflammation-mediated molecular events related to multistep carcinogenesis. (2011)
  • Author(s): Prakobwong S, Khoontawad J, Yongvanit P, Pairojkul C, Hiraku Y, Sithithaworn P, Pinlaor P, Aggarwal BB, Pinlaor S
  • Journal Title: Int. J. Cancer Volume: 129 Pages: 88-100
  • DOI: DOI:10.1002/ijc.25656.
  • Peer Reviewed
• [Journal Article] Curcumin suppresses proliferation and induces apoptosis in human biliary cancer cells through modulation of multiple cell signaling pathways (2011)
  • Author(s): Prakobwong S, Gupta SC, Kim JH, Sung B, Pinlaor P, Hiraku Y, Wongkham S, Sripa B, Pinlaor S, Aggarwal BB
  • Journal Title: Carcinogenesis Volume: 32 Pages: 1372-1380
  • DOI: DOI:10.1093/carcin/bgr032
  • Peer Reviewed
• [Journal Article] Nitrative DNA damage and Oct3/4 expression in urinary bladder cancer with Schistosomahaematobium infection (2011)
  • Author(s): Ma N, Thanan R, Kobayashi H, Hammam O, Wishahi M, Leithy TE, Hiraku Y, Amro el K, Oikawa S, Ohnishi S, Murata M, Kawanishi S
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 414 Pages: 344-349
  • DOI: DOI:10.1016/j.bbrc.2011.09.073
  • Peer Reviewed
• [Journal Article] Nitrative and oxidative DNA damage caused by K-ras mutation in mice (2011)
  • Author(s): Ohnishi S, Saito H, Suzuki N, Ma N, Hiraku Y, Murata M, Kawanishi S
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 413 Pages: 236-240
  • DOI: DOI:10.1016/ j.bbrc.2011.08.076
  • Peer Reviewed
• [Presentation] 多層カーボンナノチューブによる細胞内ニトロ化DNA損傷: 酸化DNA損傷との比較 (2013)
  • Author(s): Feiye Guo、馬寧、川西正祐、村田真理子、平工雄介
  • Organizer: 第83回日本衛生学会総会
  • Place of Presentation: 金沢市
  • Year and Date: 20130324-26
• [Presentation] 多層カーボンナノチューブによるDNA損傷:肺上皮細胞における8-ニトログアニン生成 (2013)
  • Author(s): 平工雄介、Feiye Guo、馬寧、川西正祐、村田真理子
  • Organizer: 第12回分子予防環境医学研究会
  • Place of Presentation: つくば市
  • Year and Date: 20130201-02
• [Presentation] カーボンナノチューブによる細胞内ニトロ化DNA損傷 (2012)
  • Author(s): 平工雄介、Feiye Guo、馬寧、川西正祐、村田真理子
  • Organizer: フォーラム2012衛生薬学・環境トキシコロジー
  • Place of Presentation: フォーラム2012衛生薬学・環境トキシコロジー
  • Year and Date: 20121025-26
• [Presentation] ヒト肺における石綿繊維量と酸化・ニトロ化DNA損傷との関連 (2012)
  • Author(s): 平工雄介、馬寧、川西正祐、村田真理子
  • Organizer: 第71回日本癌学会総会
  • Place of Presentation: 札幌市
  • Year and Date: 20120919-21
• [Presentation] Nitrative DNA damage in human lung tissues in association with asbestos exposure, The 7th International Conference on the Biology (2012)
  • Author(s): Yusuke Hiraku, Kiyoshi Sakai, Eiji Shibata, Michihiro Kamijima, Naomi Hisanaga, Ning Ma, Shosuke Kawanishi, Mariko Murata
  • Organizer: Chemistry and Therapeutic Applications of Nitric Oxide
  • Place of Presentation: Edinburgh, UK
  • Year and Date: 20120722-26
• [Presentation] Nitrative DNA damage in human lung epithelial cells treated with multi-walled carbon nanotube, The 7th International Conference on the Biology (2012)
  • Author(s): Feiye Guo, Ning Ma, Shosuke Kawanishi, Mariko Murata, Yusuke Hiraku
  • Organizer: Chemistry and Therapeutic Applications of Nitric Oxide
  • Place of Presentation: Edinburgh, UK
  • Year and Date: 20120722-26
• [Presentation] 多層カーボンナノチューブによる細胞内8-ニトログアニン生成 (2012)
  • Author(s): 平工雄介、Feiye Guo、馬寧、川西正祐、村田真理子
  • Organizer: 第12回日本NO学会学術集会
  • Place of Presentation: 神戸市
  • Year and Date: 20120629-30
• [Presentation] ヒト肺組織における石綿繊維量と酸化・ニトロ化DNA損傷およびDNA修復酵素発現との関連 (2012)
  • Author(s): 平工雄介、酒井潔、柴田英治、上島通浩、久永直見、村田真理子
  • Organizer: 第85回日本産業衛生学会
  • Place of Presentation: 名古屋市
  • Year and Date: 20120530-0602
• [Presentation] ヒト肺組織における石綿繊維量とDNA損傷との関連 (2012)
  • Author(s): 平工雄介、酒井潔、柴田英治、上島通浩、久永直見、馬寧、川西正祐、村田真理子
  • Organizer: 第82回日本衛生学会総会
  • Place of Presentation: 京都市
  • Year and Date: 20120324-26
• [Presentation] 感染・炎症関連発がんにおける酸 化・ニ ト ロ 化DNA損傷:その役割と意義 (2012)
  • Author(s): 平工雄介
  • Organizer: 日本薬学会東海支部特別講演会
  • Place of Presentation: 名古屋市(招待講演)
  • Year and Date: 2012-01-24
• [Presentation] 多層カーボンナノチューブで処理したヒト肺上皮細胞におけるニトロ化DNA損傷 (2011)
  • Author(s): Feiye Guo、馬寧、川西正祐、村田真理子、平工雄介
  • Organizer: 第70回日本癌学会総会
  • Place of Presentation: 名古屋市
  • Year and Date: 20111003-05
• [Presentation] ヒト肺組織における酸化・ニトロ化DNA損傷と石綿曝露との関 連 (2011)
  • Author(s): 平工雄介、酒井潔、柴田英治、上島通浩、久永直見、村田真理子
  • Organizer: 第84回日本産業衛生学会(優秀演題)
  • Place of Presentation: 東京
  • Year and Date: 20110518-20
• [Presentation] ヒト肺上皮細胞におけるカーボンブラックナノ粒子によるニトロ化DNA損傷 (2011)
  • Author(s): 平工雄介、西川嘉広、馬寧、川西正祐、村田真理子
  • Organizer: 第11回日本NO学会学術集会
  • Place of Presentation: 町田市
  • Year and Date: 20110513-14
• [Book] Role of chronic inflammation and resulting DNA damage in cervical carcinogenesis induced by human papillomavirus, In: Human Papillomavirus and Related Diseases -From Bench to Bedside - Research aspects,Broeck DV (ed.) (2012)
  • Author(s): Hiraku Y
  • Total Pages: 359-382
  • Publisher: InTech
• [Book] 8-Nitroguanine: A potential biomarker to evaluate the risk of inflammationrelated carcinogenesis, In Biomarker, Kahn TK (Ed.) (2012)
  • Author(s): Ma N, Murata M, Ohnishi S, Thanan R, Hiraku Y, Kawanishi S
  • Total Pages: 201-224
  • Publisher: InTech
• [Remarks] ホームページ: 三重大学大学院医学系研究科環境分子医学分野
  • URL: http://www.medic.mie-u.ac.jp/eiseigaku/