2013 Fiscal Year Final Research Report
Development of detection method for bacteria in valiable but not culturable state
| Project/Area Number |
23659334
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hygiene
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
鳥羽 衛 福島県立医科大学, 医学部, 助教 (20443864)
佐藤 友香 福島県立医科大学, 医学部, 助教 (70583623)
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| Co-Investigator(Renkei-kenkyūsha) |
福島 哲仁 福島県立医科大学, 医学部, 教授 (90208942)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 食品衛生 / 細菌性食中毒 |
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| Outline of Final Research Achievements |
After suspension of E. coli in sterilized distilled water, we incubated this suspension at 4 or 20 degrees to detect the culturable (or colony forming) E. coli titer continuously. 1) Using agar media, culturable E. coli was detected for 120-160 or 210-230 days from incubation at 4 or 20 degree, respectively. However, culturable E. coli was detected for an additional 30-40 days using liquid media. This result indicates that a judgment regarding the viable but not culturable (VBNC) state of bacteria should be carried out with liquid media. 2) We incubated the E. coli suspension for a further month after judging it to be in a VBNC state and injected the VBNC E. coli into 6 mice per os. The recovery of E. coli from a VBNC to a culturable state in mice was detected in the excrement from 2 of 6 mice. This result clarified that E. coli could form a true VBNC state.
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| Free Research Field |
医歯薬学
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