2012 Fiscal Year Final Research Report
Search for novel peptide hormones, which act on gastrointestinal stemcells, and its role on the regeneration of gastrointestinal epithelium
| Project/Area Number |
23659405
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | Kurume University |
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Principal Investigator |
KOJIMA Masayasu 久留米大学, 分子生命科学研究所, 教授 (20202062)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 下部消化管学(小腸、大腸)、 / 消化管幹細胞 |
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| Research Abstract |
An orphan GPCR LGR5 receptor is a marker for gastrointestinal stem cells. However,the endogenous ligand for LGR5 has not been discovered. We established a cell line, whichstably expressed LGR5, and used it for a systematic assay on brain and gastrointestinaltissue peptide extracts to search for the ligands. We detected a positive signal in thebrain samples and purified it to a single peptide. By amino acid sequence analysis, wefound that the purified peptide is CGRP, a neuropeptide. We thought that CGRP should havereacted to endogenous CGRP receptors on CHO cells. The real endogenous ligands for LGR5and its relative receptors, LGR4 and LGR6, are still controversial.
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[Journal Article] Isolation of thebioactive peptides CCHamide-1 andCCHamide-2 from Drosophila and theirputative role in appetite regulation as ligands for G protein-coupled receptors.2012
Author(s)
Ida T, Takahashi T, Tominaga H, SatoT, Sano H, Kume K, Ozaki M, Hiraguchi T, Shiotani H, Terajima S, Nakamura Y, Mori K,Yoshida M, Kato J, Murakami N, Miyazato M,Kangawa K, Kojima M.
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Journal Title
Front Endocrinol (Lausanne).
Volume: 3
Pages: 177
DOI
Peer Reviewed
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[Journal Article] Identification of the endogenouscysteine-rich peptide trissin, a ligandfor an orphan G protein-coupled receptor inDrosophila.2011
Author(s)
Ida T, Takahashi T, Tominaga H, SatoT, Kume K, Yoshizawa-Kumagaye K, N i s h i o H, Kato J, Murakami N, Miyazato M, Kangawa K,Kojima M.
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Journal Title
Biochem Biophys Res Commun.
Volume: 414(1)
Pages: 44-8
DOI
Peer Reviewed
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[Journal Article] Identification of thenovel bioactive peptides dRYamide-1 and dRYamide-2, ligands for a neuropeptideY-like receptor in Drosophila.2011
Author(s)
Ida T, Takahashi T, Tominaga H, SatoT, Kume K, Ozaki M, Hiraguchi T, Maeda T,Shiotani H, Terajima S, Sano H, Mori K,Yoshida M, Miyazato M, Kato J, Murakami N,Kangawa K, Kojima M.
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Journal Title
BiochemBiophys Res Commun.
Volume: 410(4)
Pages: 872-7
DOI
Peer Reviewed
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