2013 Fiscal Year Final Research Report
Exhaustive screening of novel genes responsible for refractory heart diseases
| Project/Area Number |
23659410
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 分子心臓病態学 / 心臓病 / 遺伝 / 遺伝性不整脈 |
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| Research Abstract |
We performed a large-scale forward genetic screening for the random mutated mice library based on the functional, morphological, and electrophysiological parameters to find new model with genetic cardiac disorders. First screening of approximately 6,800 mice have been completed, 41 of them were determined as having positive phenotype, and second generation were produced by crossing between 29 mice of phenotype-positive mice. Among them, 2 strains (1 strain with left ventricular hypertrophy and 1 strain with ventricular arrhythmia) had inheritable characters. We identified a genetic locus responsible for arrhythmic phenotype (polymorphic ventricular tachycardia) using SNP mapping. We will identify the responsible gene using next-generation sequencing, analyze detailed phenotypes, and report a novel model of hereditary arrhythmia near future.
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