2012 Fiscal Year Final Research Report
Comprehensive establishment of human immortalized cell lines originating from central and peripheral nervous system
| Project/Area Number |
23659457
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
KANDA Takashi 山口大学, 大学院・医学系研究科, 教授 (40204797)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 神経細胞学 / 神経免疫学 / 血液脊髄関門 / 不死化細胞株 / 内皮細胞 / 血管周細胞 |
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| Research Abstract |
Human immortalized cell lines originating from central and peripheral nervous system are potent tool to understand the molecular and physiological properties of cells composing the human nervous system. The aim of this study is to comprehensively establish the human cell lines of central and peripheral nervous system origin which retain original cellular properties. In this study period, we finally established (1) microvascular endothelial cell line and pericyte cell line of human skeletal muscle, and (2) microvascular endothelial cell line and pericyte cell line of human spinal cord. These immortalized cell lines adequately keep cellular properties of cells in vivo, and thus will be widely used as potent research tools for the human muscle disorders and spinal cord diseases including multiple sclerosis and neuromyelitis optica.
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[Journal Article] A stable human brain microvascular endothelial cell line retaining its barrier-specific nature, independent of the passage number2013
Author(s)
Yasuteru Sano, Yoko Kashiwamura, Masaaki Abe, Le-Ha Dieu, Jorg Huwyler, Fumitaka Shimizu, Hiroyo Haruki, Toshihiko Maeda, Kazuyuki Saito, Ayako Tasaki, Takashi Kanda
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Journal Title
Clin Exp Neuroimmunol
Volume: 4(in press)
Peer Reviewed
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[Journal Article] Establishment and Characterization of Human Peripheral Nerve Microvascular Endothelial Cell Lines: A New in vitroBlood-Nerve Barrier (BNB) Model2012
Author(s)
Masaaki Abe, Yasuteru Sano, Toshihiko Maeda, Fumitaka Shimizu, Yoko Kashiwamura, Hiroyo Haruki, Kazuyuki Saito, Ayako Tasaki, Motoharu Kawai, Tetsuya Terasaki, Takashi Kanda
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Journal Title
Cell Struct Funct
Volume: 37
Pages: 89-100
DOI
Peer Reviewed
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